The increase in cytosolic levels of protein kinase C precedes the growth factor requirements during the transition stem cells to differentiated progeny in the MCF-7 breast tumor cells.

The expression of regulatory genes within the context of a differentiation program can have profound long-term consequences in tissues with permanent renewing populations. The breast tumor cell line MCF-7 retains in culture some of the characteristics of a unidirectional differentiation pathway. We...

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Autores principales: Gómez, María Laura, Resnicoff, Mariana
Publicado: 1993
Materias:
growth factor
protein kinase c
article
breast tumor
cell differentiation
cell maturation
cell subpopulation
cytosol
enzyme activity
gene expression
human
human cell
priority journal
regulator gene
stem cell
tumor cell line
Breast Neoplasms
Cell Differentiation
Cytosol
Female
Genes, Regulator
Growth Substances
Human
Protein Kinase C
Tumor Cells, Cultured
Tumor Stem Cells
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10656995_v17_n5_p495_Gomez
http://hdl.handle.net/20.500.12110/paper_10656995_v17_n5_p495_Gomez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_10656995_v17_n5_p495_Gomez
record_format dspace
spelling paper:paper_10656995_v17_n5_p495_Gomez2023-06-08T16:04:14Z The increase in cytosolic levels of protein kinase C precedes the growth factor requirements during the transition stem cells to differentiated progeny in the MCF-7 breast tumor cells. Gómez, María Laura Resnicoff, Mariana growth factor protein kinase c article breast tumor cell differentiation cell maturation cell subpopulation cytosol enzyme activity gene expression human human cell priority journal regulator gene stem cell tumor cell line Breast Neoplasms Cell Differentiation Cytosol Female Genes, Regulator Growth Substances Human Protein Kinase C Tumor Cells, Cultured Tumor Stem Cells The expression of regulatory genes within the context of a differentiation program can have profound long-term consequences in tissues with permanent renewing populations. The breast tumor cell line MCF-7 retains in culture some of the characteristics of a unidirectional differentiation pathway. We show that the cytosolic activity of the regulatory enzyme protein kinase C (PKC) precedes and continues the sequence of maturation in pre-differentiated subpopulations derived from a stem cell fraction. However, the activity declines in the most differentiated, post-mitotic fraction. These results indicate that PKC may be considered among the regulatory genes in MCF-7 cells that specify maturation of the stem cell progeny. © 1993 Academic Press. All rights reserved. Fil:Gomez, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Resnicoff, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10656995_v17_n5_p495_Gomez http://hdl.handle.net/20.500.12110/paper_10656995_v17_n5_p495_Gomez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic growth factor
protein kinase c
article
breast tumor
cell differentiation
cell maturation
cell subpopulation
cytosol
enzyme activity
gene expression
human
human cell
priority journal
regulator gene
stem cell
tumor cell line
Breast Neoplasms
Cell Differentiation
Cytosol
Female
Genes, Regulator
Growth Substances
Human
Protein Kinase C
Tumor Cells, Cultured
Tumor Stem Cells
spellingShingle growth factor
protein kinase c
article
breast tumor
cell differentiation
cell maturation
cell subpopulation
cytosol
enzyme activity
gene expression
human
human cell
priority journal
regulator gene
stem cell
tumor cell line
Breast Neoplasms
Cell Differentiation
Cytosol
Female
Genes, Regulator
Growth Substances
Human
Protein Kinase C
Tumor Cells, Cultured
Tumor Stem Cells
Gómez, María Laura
Resnicoff, Mariana
The increase in cytosolic levels of protein kinase C precedes the growth factor requirements during the transition stem cells to differentiated progeny in the MCF-7 breast tumor cells.
topic_facet growth factor
protein kinase c
article
breast tumor
cell differentiation
cell maturation
cell subpopulation
cytosol
enzyme activity
gene expression
human
human cell
priority journal
regulator gene
stem cell
tumor cell line
Breast Neoplasms
Cell Differentiation
Cytosol
Female
Genes, Regulator
Growth Substances
Human
Protein Kinase C
Tumor Cells, Cultured
Tumor Stem Cells
description The expression of regulatory genes within the context of a differentiation program can have profound long-term consequences in tissues with permanent renewing populations. The breast tumor cell line MCF-7 retains in culture some of the characteristics of a unidirectional differentiation pathway. We show that the cytosolic activity of the regulatory enzyme protein kinase C (PKC) precedes and continues the sequence of maturation in pre-differentiated subpopulations derived from a stem cell fraction. However, the activity declines in the most differentiated, post-mitotic fraction. These results indicate that PKC may be considered among the regulatory genes in MCF-7 cells that specify maturation of the stem cell progeny. © 1993 Academic Press. All rights reserved.
author Gómez, María Laura
Resnicoff, Mariana
author_facet Gómez, María Laura
Resnicoff, Mariana
author_sort Gómez, María Laura
title The increase in cytosolic levels of protein kinase C precedes the growth factor requirements during the transition stem cells to differentiated progeny in the MCF-7 breast tumor cells.
title_short The increase in cytosolic levels of protein kinase C precedes the growth factor requirements during the transition stem cells to differentiated progeny in the MCF-7 breast tumor cells.
title_full The increase in cytosolic levels of protein kinase C precedes the growth factor requirements during the transition stem cells to differentiated progeny in the MCF-7 breast tumor cells.
title_fullStr The increase in cytosolic levels of protein kinase C precedes the growth factor requirements during the transition stem cells to differentiated progeny in the MCF-7 breast tumor cells.
title_full_unstemmed The increase in cytosolic levels of protein kinase C precedes the growth factor requirements during the transition stem cells to differentiated progeny in the MCF-7 breast tumor cells.
title_sort increase in cytosolic levels of protein kinase c precedes the growth factor requirements during the transition stem cells to differentiated progeny in the mcf-7 breast tumor cells.
publishDate 1993
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10656995_v17_n5_p495_Gomez
http://hdl.handle.net/20.500.12110/paper_10656995_v17_n5_p495_Gomez
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AT resnicoffmariana theincreaseincytosoliclevelsofproteinkinasecprecedesthegrowthfactorrequirementsduringthetransitionstemcellstodifferentiatedprogenyinthemcf7breasttumorcells
AT gomezmarialaura increaseincytosoliclevelsofproteinkinasecprecedesthegrowthfactorrequirementsduringthetransitionstemcellstodifferentiatedprogenyinthemcf7breasttumorcells
AT resnicoffmariana increaseincytosoliclevelsofproteinkinasecprecedesthegrowthfactorrequirementsduringthetransitionstemcellstodifferentiatedprogenyinthemcf7breasttumorcells
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