Determining the virus life-cycle stage blocked by an antiviral

Among the members of the Arenaviridae family, Junín virus and Lassa virus represent important human health threats generating annual outbreaks of severe human hemorrhagic fever (HF) in endemic areas of Argentina and Western Africa, respectively. Given the lack of a specific and safe chemotherapy, th...

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Publicado:	2018
Materias:	Antiviral compound Antiviral mechanism Arenaviruses antivirus agent complementary DNA Arenaviridae cytopathogenic effect cytotoxicity cytotoxicity assay disease severity DNA synthesis drug mechanism EC50 endemic disease hemorrhagic fever high throughput screening human in vitro study Junin virus Lassa virus life cycle stage MTS assay MTT assay nonhuman polyacrylamide gel electrophoresis reverse transcription polymerase chain reaction RNA synthesis structure activity relation virogenesis virus assembly virus attachment virus culture virus like agent virus particle virus release virus replication virus transcription virus uncoating Western blotting drug effect Hantaan virus physiology Antiviral Agents Arenavirus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1604_n_p371_Sepulveda http://hdl.handle.net/20.500.12110/paper_10643745_v1604_n_p371_Sepulveda
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10643745_v1604_n_p371_Sepulveda
record_format	dspace
spelling	paper:paper_10643745_v1604_n_p371_Sepulveda2023-06-08T16:04:07Z Determining the virus life-cycle stage blocked by an antiviral Antiviral compound Antiviral mechanism Arenaviruses antivirus agent complementary DNA antivirus agent Arenaviridae cytopathogenic effect cytotoxicity cytotoxicity assay disease severity DNA synthesis drug mechanism EC50 endemic disease hemorrhagic fever high throughput screening human in vitro study Junin virus Lassa virus life cycle stage MTS assay MTT assay nonhuman polyacrylamide gel electrophoresis reverse transcription polymerase chain reaction RNA synthesis structure activity relation virogenesis virus assembly virus attachment virus culture virus like agent virus particle virus release virus replication virus transcription virus uncoating Western blotting Arenaviridae drug effect Hantaan virus physiology Antiviral Agents Arenavirus Hantaan virus Among the members of the Arenaviridae family, Junín virus and Lassa virus represent important human health threats generating annual outbreaks of severe human hemorrhagic fever (HF) in endemic areas of Argentina and Western Africa, respectively. Given the lack of a specific and safe chemotherapy, the search for effective antiviral compounds is a continuous demanding effort. During the last two decades, academic research studies originated important results identifying novel molecules to be considered for further in vivo characterization. This chapter summarizes experimental in vitro approaches used to determine the possible mechanism of action of these antiviral agents. © Springer Science+Business Media LLC 2018. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1604_n_p371_Sepulveda http://hdl.handle.net/20.500.12110/paper_10643745_v1604_n_p371_Sepulveda
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antiviral compound Antiviral mechanism Arenaviruses antivirus agent complementary DNA antivirus agent Arenaviridae cytopathogenic effect cytotoxicity cytotoxicity assay disease severity DNA synthesis drug mechanism EC50 endemic disease hemorrhagic fever high throughput screening human in vitro study Junin virus Lassa virus life cycle stage MTS assay MTT assay nonhuman polyacrylamide gel electrophoresis reverse transcription polymerase chain reaction RNA synthesis structure activity relation virogenesis virus assembly virus attachment virus culture virus like agent virus particle virus release virus replication virus transcription virus uncoating Western blotting Arenaviridae drug effect Hantaan virus physiology Antiviral Agents Arenavirus Hantaan virus
spellingShingle	Antiviral compound Antiviral mechanism Arenaviruses antivirus agent complementary DNA antivirus agent Arenaviridae cytopathogenic effect cytotoxicity cytotoxicity assay disease severity DNA synthesis drug mechanism EC50 endemic disease hemorrhagic fever high throughput screening human in vitro study Junin virus Lassa virus life cycle stage MTS assay MTT assay nonhuman polyacrylamide gel electrophoresis reverse transcription polymerase chain reaction RNA synthesis structure activity relation virogenesis virus assembly virus attachment virus culture virus like agent virus particle virus release virus replication virus transcription virus uncoating Western blotting Arenaviridae drug effect Hantaan virus physiology Antiviral Agents Arenavirus Hantaan virus Determining the virus life-cycle stage blocked by an antiviral
topic_facet	Antiviral compound Antiviral mechanism Arenaviruses antivirus agent complementary DNA antivirus agent Arenaviridae cytopathogenic effect cytotoxicity cytotoxicity assay disease severity DNA synthesis drug mechanism EC50 endemic disease hemorrhagic fever high throughput screening human in vitro study Junin virus Lassa virus life cycle stage MTS assay MTT assay nonhuman polyacrylamide gel electrophoresis reverse transcription polymerase chain reaction RNA synthesis structure activity relation virogenesis virus assembly virus attachment virus culture virus like agent virus particle virus release virus replication virus transcription virus uncoating Western blotting Arenaviridae drug effect Hantaan virus physiology Antiviral Agents Arenavirus Hantaan virus
description	Among the members of the Arenaviridae family, Junín virus and Lassa virus represent important human health threats generating annual outbreaks of severe human hemorrhagic fever (HF) in endemic areas of Argentina and Western Africa, respectively. Given the lack of a specific and safe chemotherapy, the search for effective antiviral compounds is a continuous demanding effort. During the last two decades, academic research studies originated important results identifying novel molecules to be considered for further in vivo characterization. This chapter summarizes experimental in vitro approaches used to determine the possible mechanism of action of these antiviral agents. © Springer Science+Business Media LLC 2018.
title	Determining the virus life-cycle stage blocked by an antiviral
title_short	Determining the virus life-cycle stage blocked by an antiviral
title_full	Determining the virus life-cycle stage blocked by an antiviral
title_fullStr	Determining the virus life-cycle stage blocked by an antiviral
title_full_unstemmed	Determining the virus life-cycle stage blocked by an antiviral
title_sort	determining the virus life-cycle stage blocked by an antiviral
publishDate	2018
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1604_n_p371_Sepulveda http://hdl.handle.net/20.500.12110/paper_10643745_v1604_n_p371_Sepulveda
_version_	1768545017448628224

Determining the virus life-cycle stage blocked by an antiviral

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