Optimized method for growing in vitro Arabidopsis thaliana pollen tubes
Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tube...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1242_n_p41_Borassi http://hdl.handle.net/20.500.12110/paper_10643745_v1242_n_p41_Borassi |
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paper:paper_10643745_v1242_n_p41_Borassi2023-06-08T16:04:05Z Optimized method for growing in vitro Arabidopsis thaliana pollen tubes Arabidopsis pollen Cell expansion Pollen tube growth calcium ion reactive oxygen metabolite Arabidopsis protein bacterial DNA T-DNA Arabidopsis thaliana Article assay controlled study gene insertion growth regulation in vitro study mutant nonhuman phenotype plant growth pollen germination pollen tube pollen tube growth Arabidopsis botany cell polarity embryology gene expression regulation genetics germination growth, development and aging mutation physiology procedures Arabidopsis Arabidopsis thaliana Arabidopsis Arabidopsis Proteins Botany Cell Polarity DNA, Bacterial Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination Mutation Pollen Tube Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed. © Springer Science+Business Media New York 2015. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1242_n_p41_Borassi http://hdl.handle.net/20.500.12110/paper_10643745_v1242_n_p41_Borassi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arabidopsis pollen Cell expansion Pollen tube growth calcium ion reactive oxygen metabolite Arabidopsis protein bacterial DNA T-DNA Arabidopsis thaliana Article assay controlled study gene insertion growth regulation in vitro study mutant nonhuman phenotype plant growth pollen germination pollen tube pollen tube growth Arabidopsis botany cell polarity embryology gene expression regulation genetics germination growth, development and aging mutation physiology procedures Arabidopsis Arabidopsis thaliana Arabidopsis Arabidopsis Proteins Botany Cell Polarity DNA, Bacterial Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination Mutation Pollen Tube |
| spellingShingle |
Arabidopsis pollen Cell expansion Pollen tube growth calcium ion reactive oxygen metabolite Arabidopsis protein bacterial DNA T-DNA Arabidopsis thaliana Article assay controlled study gene insertion growth regulation in vitro study mutant nonhuman phenotype plant growth pollen germination pollen tube pollen tube growth Arabidopsis botany cell polarity embryology gene expression regulation genetics germination growth, development and aging mutation physiology procedures Arabidopsis Arabidopsis thaliana Arabidopsis Arabidopsis Proteins Botany Cell Polarity DNA, Bacterial Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination Mutation Pollen Tube Optimized method for growing in vitro Arabidopsis thaliana pollen tubes |
| topic_facet |
Arabidopsis pollen Cell expansion Pollen tube growth calcium ion reactive oxygen metabolite Arabidopsis protein bacterial DNA T-DNA Arabidopsis thaliana Article assay controlled study gene insertion growth regulation in vitro study mutant nonhuman phenotype plant growth pollen germination pollen tube pollen tube growth Arabidopsis botany cell polarity embryology gene expression regulation genetics germination growth, development and aging mutation physiology procedures Arabidopsis Arabidopsis thaliana Arabidopsis Arabidopsis Proteins Botany Cell Polarity DNA, Bacterial Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination Mutation Pollen Tube |
| description |
Pollen tubes elongate by tip growth toward the ovule to deliver the sperm cells during fertilization. Since pollen tubes from several species can be grown in vitro maintaining their polarity, pollen tube growth is a suitable model system to study cell polarity and tip growth. A. thaliana pollen tubes germinated in vitro for 6 h can reach up to 800 μm. By studying the phenotype of mutants of T-DNA insertion lines, genes involved in pollen tube growth can be identified. Moreover, components involved in the regulation of pollen tube growth such as calcium ions and reactive oxygen species (ROS) can be analyzed. © Springer Science+Business Media New York 2015. |
| title |
Optimized method for growing in vitro Arabidopsis thaliana pollen tubes |
| title_short |
Optimized method for growing in vitro Arabidopsis thaliana pollen tubes |
| title_full |
Optimized method for growing in vitro Arabidopsis thaliana pollen tubes |
| title_fullStr |
Optimized method for growing in vitro Arabidopsis thaliana pollen tubes |
| title_full_unstemmed |
Optimized method for growing in vitro Arabidopsis thaliana pollen tubes |
| title_sort |
optimized method for growing in vitro arabidopsis thaliana pollen tubes |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10643745_v1242_n_p41_Borassi http://hdl.handle.net/20.500.12110/paper_10643745_v1242_n_p41_Borassi |
| _version_ |
1768545428361445376 |