Promoter shape varies across populations and affects promoter evolution and expression noise
Animal promoters initiate transcription either at precise positions (narrow promoters) or dispersed regions (broad promoters), a distinction referred to as promoter shape. Although highly conserved, the functional properties of promoters with different shapes and the genetic basis of their evolution...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10614036_v49_n4_p550_Schor http://hdl.handle.net/20.500.12110/paper_10614036_v49_n4_p550_Schor |
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paper:paper_10614036_v49_n4_p550_Schor2023-06-08T16:03:29Z Promoter shape varies across populations and affects promoter evolution and expression noise Article cytometry Drosophila genetic variation noise nonhuman priority journal promoter region single cell analysis animal evolution genetic transcription genetics noise physiology promoter region transcription initiation site Animals Biological Evolution Drosophila Genetic Variation Noise Promoter Regions, Genetic Transcription Initiation Site Transcription, Genetic Animal promoters initiate transcription either at precise positions (narrow promoters) or dispersed regions (broad promoters), a distinction referred to as promoter shape. Although highly conserved, the functional properties of promoters with different shapes and the genetic basis of their evolution remain unclear. Here we used natural genetic variation across a panel of 81 Drosophila lines to measure changes in transcriptional start site (TSS) usage, identifying thousands of genetic variants affecting transcript levels (strength) or the distribution of TSSs within a promoter (shape). Our results identify promoter shape as a molecular trait that can evolve independently of promoter strength. Broad promoters typically harbor shape-associated variants, with signatures of adaptive selection. Single-cell measurements demonstrate that variants modulating promoter shape often increase expression noise, whereas heteroallelic interactions with other promoter variants alleviate these effects. These results uncover new functional properties of natural promoters and suggest the minimization of expression noise as an important factor in promoter evolution. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10614036_v49_n4_p550_Schor http://hdl.handle.net/20.500.12110/paper_10614036_v49_n4_p550_Schor |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Article cytometry Drosophila genetic variation noise nonhuman priority journal promoter region single cell analysis animal evolution genetic transcription genetics noise physiology promoter region transcription initiation site Animals Biological Evolution Drosophila Genetic Variation Noise Promoter Regions, Genetic Transcription Initiation Site Transcription, Genetic |
| spellingShingle |
Article cytometry Drosophila genetic variation noise nonhuman priority journal promoter region single cell analysis animal evolution genetic transcription genetics noise physiology promoter region transcription initiation site Animals Biological Evolution Drosophila Genetic Variation Noise Promoter Regions, Genetic Transcription Initiation Site Transcription, Genetic Promoter shape varies across populations and affects promoter evolution and expression noise |
| topic_facet |
Article cytometry Drosophila genetic variation noise nonhuman priority journal promoter region single cell analysis animal evolution genetic transcription genetics noise physiology promoter region transcription initiation site Animals Biological Evolution Drosophila Genetic Variation Noise Promoter Regions, Genetic Transcription Initiation Site Transcription, Genetic |
| description |
Animal promoters initiate transcription either at precise positions (narrow promoters) or dispersed regions (broad promoters), a distinction referred to as promoter shape. Although highly conserved, the functional properties of promoters with different shapes and the genetic basis of their evolution remain unclear. Here we used natural genetic variation across a panel of 81 Drosophila lines to measure changes in transcriptional start site (TSS) usage, identifying thousands of genetic variants affecting transcript levels (strength) or the distribution of TSSs within a promoter (shape). Our results identify promoter shape as a molecular trait that can evolve independently of promoter strength. Broad promoters typically harbor shape-associated variants, with signatures of adaptive selection. Single-cell measurements demonstrate that variants modulating promoter shape often increase expression noise, whereas heteroallelic interactions with other promoter variants alleviate these effects. These results uncover new functional properties of natural promoters and suggest the minimization of expression noise as an important factor in promoter evolution. |
| title |
Promoter shape varies across populations and affects promoter evolution and expression noise |
| title_short |
Promoter shape varies across populations and affects promoter evolution and expression noise |
| title_full |
Promoter shape varies across populations and affects promoter evolution and expression noise |
| title_fullStr |
Promoter shape varies across populations and affects promoter evolution and expression noise |
| title_full_unstemmed |
Promoter shape varies across populations and affects promoter evolution and expression noise |
| title_sort |
promoter shape varies across populations and affects promoter evolution and expression noise |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10614036_v49_n4_p550_Schor http://hdl.handle.net/20.500.12110/paper_10614036_v49_n4_p550_Schor |
| _version_ |
1768545749007597568 |