Single photon fluorescent microlithography for live-cell imaging
Using fluorescent dyes to trigger the polymerization of a commercial polyurethane resin allows a rapid fabrication of micrometer and submicrometer sized fluorescent structures by one-photon absorption. Here, we show that standard He-Ne lasers emitting at 632.8 nm can be used to start the photopolyme...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v73_n1_p20_Kunik http://hdl.handle.net/20.500.12110/paper_1059910X_v73_n1_p20_Kunik |
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paper:paper_1059910X_v73_n1_p20_Kunik2023-06-08T16:03:26Z Single photon fluorescent microlithography for live-cell imaging Kunik, Darío Aramendía, Pedro Francisco Martínez, Oscar Eduardo Colocalization Lithography Microfluidics Microscopy Photopolymerization Animals Cells, Cultured Laser Therapy, Low-Level Melanophores Microscopy, Confocal Microscopy, Fluorescence Polyurethanes Urethane Xenopus Using fluorescent dyes to trigger the polymerization of a commercial polyurethane resin allows a rapid fabrication of micrometer and submicrometer sized fluorescent structures by one-photon absorption. Here, we show that standard He-Ne lasers emitting at 632.8 nm can be used to start the photopolymerization and that very low laser power is required. This procedure allows the fabrication of fiduciary fluorescent references on standard glass coverslips, mica sheets, or gold-coated coverslips for laser scanning or standard fluorescent microscopy. The biocompatibility of the polymerized resin with cells in culture was tested by growing Xenopus melanophores and a standard laser scanning microscope was used to demonstrate that it is possible to use equipment readily available in several laboratories. We show that fluorescent structure with less than 10 nm in height may be used as references in fluorescence microscopy allowing a smooth environment for cell growth. Different dyes were tested and the conditions for one-photon polymerization were outlined. © 2009 Wiley-Liss, Inc. Fil:Kunik, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Aramendia, P.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martínez, O.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v73_n1_p20_Kunik http://hdl.handle.net/20.500.12110/paper_1059910X_v73_n1_p20_Kunik |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Colocalization Lithography Microfluidics Microscopy Photopolymerization Animals Cells, Cultured Laser Therapy, Low-Level Melanophores Microscopy, Confocal Microscopy, Fluorescence Polyurethanes Urethane Xenopus |
| spellingShingle |
Colocalization Lithography Microfluidics Microscopy Photopolymerization Animals Cells, Cultured Laser Therapy, Low-Level Melanophores Microscopy, Confocal Microscopy, Fluorescence Polyurethanes Urethane Xenopus Kunik, Darío Aramendía, Pedro Francisco Martínez, Oscar Eduardo Single photon fluorescent microlithography for live-cell imaging |
| topic_facet |
Colocalization Lithography Microfluidics Microscopy Photopolymerization Animals Cells, Cultured Laser Therapy, Low-Level Melanophores Microscopy, Confocal Microscopy, Fluorescence Polyurethanes Urethane Xenopus |
| description |
Using fluorescent dyes to trigger the polymerization of a commercial polyurethane resin allows a rapid fabrication of micrometer and submicrometer sized fluorescent structures by one-photon absorption. Here, we show that standard He-Ne lasers emitting at 632.8 nm can be used to start the photopolymerization and that very low laser power is required. This procedure allows the fabrication of fiduciary fluorescent references on standard glass coverslips, mica sheets, or gold-coated coverslips for laser scanning or standard fluorescent microscopy. The biocompatibility of the polymerized resin with cells in culture was tested by growing Xenopus melanophores and a standard laser scanning microscope was used to demonstrate that it is possible to use equipment readily available in several laboratories. We show that fluorescent structure with less than 10 nm in height may be used as references in fluorescence microscopy allowing a smooth environment for cell growth. Different dyes were tested and the conditions for one-photon polymerization were outlined. © 2009 Wiley-Liss, Inc. |
| author |
Kunik, Darío Aramendía, Pedro Francisco Martínez, Oscar Eduardo |
| author_facet |
Kunik, Darío Aramendía, Pedro Francisco Martínez, Oscar Eduardo |
| author_sort |
Kunik, Darío |
| title |
Single photon fluorescent microlithography for live-cell imaging |
| title_short |
Single photon fluorescent microlithography for live-cell imaging |
| title_full |
Single photon fluorescent microlithography for live-cell imaging |
| title_fullStr |
Single photon fluorescent microlithography for live-cell imaging |
| title_full_unstemmed |
Single photon fluorescent microlithography for live-cell imaging |
| title_sort |
single photon fluorescent microlithography for live-cell imaging |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v73_n1_p20_Kunik http://hdl.handle.net/20.500.12110/paper_1059910X_v73_n1_p20_Kunik |
| work_keys_str_mv |
AT kunikdario singlephotonfluorescentmicrolithographyforlivecellimaging AT aramendiapedrofrancisco singlephotonfluorescentmicrolithographyforlivecellimaging AT martinezoscareduardo singlephotonfluorescentmicrolithographyforlivecellimaging |
| _version_ |
1768546219423956992 |