Two-photon fluorescent microlithography for live-cell imaging
Fluorescent dyes added to UV-cure resins allow the rapid fabrication of fluorescent micropatterns on standard glass coverslips by two-photon optical lithography. We use this lithographic method to tailor fiduciary markers, focal references, and calibration tools, for fluorescence and laser scanning...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v68_n5_p272_Costantino http://hdl.handle.net/20.500.12110/paper_1059910X_v68_n5_p272_Costantino |
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paper:paper_1059910X_v68_n5_p272_Costantino |
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paper:paper_1059910X_v68_n5_p272_Costantino2023-06-08T16:03:26Z Two-photon fluorescent microlithography for live-cell imaging Fluorescent fiduciary marks Fluorescent lithography Microcontact printing Nonlinear optical lithography dimeticone fluorescent dye glass protein resin analytic method animal cell article biocompatibility calibration cell adhesion cell culture cell growth cell migration fluorescence hippocampus laser microscopy mammal cell nerve fiber growth newborn nonhuman priority journal quantitative analysis rat two photon fluorescent microlithography Animals Animals, Newborn Cells, Cultured CHO Cells Cricetinae Dimethylpolysiloxanes Fibronectins Fluorescent Dyes Green Fluorescent Proteins Hippocampus Humans Image Processing, Computer-Assisted Microscopy, Confocal Microscopy, Fluorescence Neurons Photons Polymers Rats Animalia Mammalia Fluorescent dyes added to UV-cure resins allow the rapid fabrication of fluorescent micropatterns on standard glass coverslips by two-photon optical lithography. We use this lithographic method to tailor fiduciary markers, focal references, and calibration tools, for fluorescence and laser scanning microscopy. Fluorescent microlithography provides spatial landmarks to quantify molecular transport, cell growth and migration, and to compensate for focal drift during time-lapse imaging. We show that the fluorescent patterned microstructures are biocompatible with cultures of mammalian cell lines and hippocampal neurons. Furthermore, the high-relief topology of the lithographed substrates is utilized as a mold for poly(dimethylsiloxane) stamps to create protein patterns by microcontact printing, representing an alternative to the current etching techniques. We present two different applications of such protein patterns for localizing cell adhesion and guidance of neunte outgrowth. © 2005 Wiley-Liss, Inc. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v68_n5_p272_Costantino http://hdl.handle.net/20.500.12110/paper_1059910X_v68_n5_p272_Costantino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fluorescent fiduciary marks Fluorescent lithography Microcontact printing Nonlinear optical lithography dimeticone fluorescent dye glass protein resin analytic method animal cell article biocompatibility calibration cell adhesion cell culture cell growth cell migration fluorescence hippocampus laser microscopy mammal cell nerve fiber growth newborn nonhuman priority journal quantitative analysis rat two photon fluorescent microlithography Animals Animals, Newborn Cells, Cultured CHO Cells Cricetinae Dimethylpolysiloxanes Fibronectins Fluorescent Dyes Green Fluorescent Proteins Hippocampus Humans Image Processing, Computer-Assisted Microscopy, Confocal Microscopy, Fluorescence Neurons Photons Polymers Rats Animalia Mammalia |
| spellingShingle |
Fluorescent fiduciary marks Fluorescent lithography Microcontact printing Nonlinear optical lithography dimeticone fluorescent dye glass protein resin analytic method animal cell article biocompatibility calibration cell adhesion cell culture cell growth cell migration fluorescence hippocampus laser microscopy mammal cell nerve fiber growth newborn nonhuman priority journal quantitative analysis rat two photon fluorescent microlithography Animals Animals, Newborn Cells, Cultured CHO Cells Cricetinae Dimethylpolysiloxanes Fibronectins Fluorescent Dyes Green Fluorescent Proteins Hippocampus Humans Image Processing, Computer-Assisted Microscopy, Confocal Microscopy, Fluorescence Neurons Photons Polymers Rats Animalia Mammalia Two-photon fluorescent microlithography for live-cell imaging |
| topic_facet |
Fluorescent fiduciary marks Fluorescent lithography Microcontact printing Nonlinear optical lithography dimeticone fluorescent dye glass protein resin analytic method animal cell article biocompatibility calibration cell adhesion cell culture cell growth cell migration fluorescence hippocampus laser microscopy mammal cell nerve fiber growth newborn nonhuman priority journal quantitative analysis rat two photon fluorescent microlithography Animals Animals, Newborn Cells, Cultured CHO Cells Cricetinae Dimethylpolysiloxanes Fibronectins Fluorescent Dyes Green Fluorescent Proteins Hippocampus Humans Image Processing, Computer-Assisted Microscopy, Confocal Microscopy, Fluorescence Neurons Photons Polymers Rats Animalia Mammalia |
| description |
Fluorescent dyes added to UV-cure resins allow the rapid fabrication of fluorescent micropatterns on standard glass coverslips by two-photon optical lithography. We use this lithographic method to tailor fiduciary markers, focal references, and calibration tools, for fluorescence and laser scanning microscopy. Fluorescent microlithography provides spatial landmarks to quantify molecular transport, cell growth and migration, and to compensate for focal drift during time-lapse imaging. We show that the fluorescent patterned microstructures are biocompatible with cultures of mammalian cell lines and hippocampal neurons. Furthermore, the high-relief topology of the lithographed substrates is utilized as a mold for poly(dimethylsiloxane) stamps to create protein patterns by microcontact printing, representing an alternative to the current etching techniques. We present two different applications of such protein patterns for localizing cell adhesion and guidance of neunte outgrowth. © 2005 Wiley-Liss, Inc. |
| title |
Two-photon fluorescent microlithography for live-cell imaging |
| title_short |
Two-photon fluorescent microlithography for live-cell imaging |
| title_full |
Two-photon fluorescent microlithography for live-cell imaging |
| title_fullStr |
Two-photon fluorescent microlithography for live-cell imaging |
| title_full_unstemmed |
Two-photon fluorescent microlithography for live-cell imaging |
| title_sort |
two-photon fluorescent microlithography for live-cell imaging |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v68_n5_p272_Costantino http://hdl.handle.net/20.500.12110/paper_1059910X_v68_n5_p272_Costantino |
| _version_ |
1768545427999686656 |