Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Förster resonance energy transfer (FRET)...

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Autores principales:	Grecco, Hernán Edgardo, Spagnuolo, Carla Cecilia, Martínez, Oscar Eduardo, Jares, Elizabeth Andrea
Publicado:	2004
Materias:	Fluorescence microscopy Growth factor receptors Nanoparticles epidermal growth factor receptor green fluorescent protein growth factor receptor hybrid protein nanoparticle quantum dot streptavidin transferrin receptor fluorescent dye nanomaterial transferrin anisotropy article cancer cell culture confocal laser microscopy fluorescence fluorescence lifetime imaging microscopy fluorescence microscopy fluorescence resonance energy transfer human human cell imaging system irradiation microscopy multiphoton microscopy photon priority journal signal transduction squamous cell carcinoma two photon excitation microscopy metabolism tumor cell line Anisotropy Cell Line, Tumor Fluorescence Resonance Energy Transfer Fluorescent Dyes Humans Microscopy, Fluorescence Nanostructures Photons Quantum Dots Receptors, Growth Factor Signal Transduction Transferrin
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v65_n4-5_p169_Grecco http://hdl.handle.net/20.500.12110/paper_1059910X_v65_n4-5_p169_Grecco
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_1059910X_v65_n4-5_p169_Grecco
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spelling	paper:paper_1059910X_v65_n4-5_p169_Grecco2023-06-08T16:03:26Z Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells Grecco, Hernán Edgardo Spagnuolo, Carla Cecilia Martínez, Oscar Eduardo Jares, Elizabeth Andrea Fluorescence microscopy Growth factor receptors Nanoparticles epidermal growth factor receptor green fluorescent protein growth factor receptor hybrid protein nanoparticle quantum dot streptavidin transferrin receptor fluorescent dye nanomaterial transferrin anisotropy article cancer cell culture confocal laser microscopy fluorescence fluorescence lifetime imaging microscopy fluorescence microscopy fluorescence resonance energy transfer human human cell imaging system irradiation microscopy multiphoton microscopy photon priority journal signal transduction squamous cell carcinoma two photon excitation microscopy fluorescence microscopy fluorescence resonance energy transfer metabolism tumor cell line Anisotropy Cell Line, Tumor Fluorescence Resonance Energy Transfer Fluorescent Dyes Humans Microscopy, Fluorescence Nanostructures Photons Quantum Dots Receptors, Growth Factor Signal Transduction Transferrin In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Förster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity illumination. We also demonstrate the utility of QDs for (1) imaging the binding and uptake of biotinylated transferrin on living cells, and (2) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs). © 2005 Wiley-Liss, Inc. Fil:Grecco, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Spagnuolo, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martinez, O.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v65_n4-5_p169_Grecco http://hdl.handle.net/20.500.12110/paper_1059910X_v65_n4-5_p169_Grecco
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Fluorescence microscopy Growth factor receptors Nanoparticles epidermal growth factor receptor green fluorescent protein growth factor receptor hybrid protein nanoparticle quantum dot streptavidin transferrin receptor fluorescent dye nanomaterial transferrin anisotropy article cancer cell culture confocal laser microscopy fluorescence fluorescence lifetime imaging microscopy fluorescence microscopy fluorescence resonance energy transfer human human cell imaging system irradiation microscopy multiphoton microscopy photon priority journal signal transduction squamous cell carcinoma two photon excitation microscopy fluorescence microscopy fluorescence resonance energy transfer metabolism tumor cell line Anisotropy Cell Line, Tumor Fluorescence Resonance Energy Transfer Fluorescent Dyes Humans Microscopy, Fluorescence Nanostructures Photons Quantum Dots Receptors, Growth Factor Signal Transduction Transferrin
spellingShingle	Fluorescence microscopy Growth factor receptors Nanoparticles epidermal growth factor receptor green fluorescent protein growth factor receptor hybrid protein nanoparticle quantum dot streptavidin transferrin receptor fluorescent dye nanomaterial transferrin anisotropy article cancer cell culture confocal laser microscopy fluorescence fluorescence lifetime imaging microscopy fluorescence microscopy fluorescence resonance energy transfer human human cell imaging system irradiation microscopy multiphoton microscopy photon priority journal signal transduction squamous cell carcinoma two photon excitation microscopy fluorescence microscopy fluorescence resonance energy transfer metabolism tumor cell line Anisotropy Cell Line, Tumor Fluorescence Resonance Energy Transfer Fluorescent Dyes Humans Microscopy, Fluorescence Nanostructures Photons Quantum Dots Receptors, Growth Factor Signal Transduction Transferrin Grecco, Hernán Edgardo Spagnuolo, Carla Cecilia Martínez, Oscar Eduardo Jares, Elizabeth Andrea Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
topic_facet	Fluorescence microscopy Growth factor receptors Nanoparticles epidermal growth factor receptor green fluorescent protein growth factor receptor hybrid protein nanoparticle quantum dot streptavidin transferrin receptor fluorescent dye nanomaterial transferrin anisotropy article cancer cell culture confocal laser microscopy fluorescence fluorescence lifetime imaging microscopy fluorescence microscopy fluorescence resonance energy transfer human human cell imaging system irradiation microscopy multiphoton microscopy photon priority journal signal transduction squamous cell carcinoma two photon excitation microscopy fluorescence microscopy fluorescence resonance energy transfer metabolism tumor cell line Anisotropy Cell Line, Tumor Fluorescence Resonance Energy Transfer Fluorescent Dyes Humans Microscopy, Fluorescence Nanostructures Photons Quantum Dots Receptors, Growth Factor Signal Transduction Transferrin
description	In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Förster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity illumination. We also demonstrate the utility of QDs for (1) imaging the binding and uptake of biotinylated transferrin on living cells, and (2) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs). © 2005 Wiley-Liss, Inc.
author	Grecco, Hernán Edgardo Spagnuolo, Carla Cecilia Martínez, Oscar Eduardo Jares, Elizabeth Andrea
author_facet	Grecco, Hernán Edgardo Spagnuolo, Carla Cecilia Martínez, Oscar Eduardo Jares, Elizabeth Andrea
author_sort	Grecco, Hernán Edgardo
title	Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
title_short	Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
title_full	Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
title_fullStr	Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
title_full_unstemmed	Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
title_sort	ensemble and single particle photophysical properties (two-photon excitation, anisotropy, fret, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells
publishDate	2004
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1059910X_v65_n4-5_p169_Grecco http://hdl.handle.net/20.500.12110/paper_1059910X_v65_n4-5_p169_Grecco
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_version_	1768543433253715968

Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

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