Zonda is a novel early component of the autophagy pathway in Drosophila
Autophagy is an evolutionary conserved process by which eukaryotic cells undergo self-digestion of cytoplasmic components. Here we report that a novel Drosophila immunophilin, which we have named Zonda, is critically required for starvation-induced autophagy. We show that Zonda operates at early sta...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10591524_v28_n22_p3070_Melani http://hdl.handle.net/20.500.12110/paper_10591524_v28_n22_p3070_Melani |
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paper:paper_10591524_v28_n22_p3070_Melani2023-06-08T16:03:24Z Zonda is a novel early component of the autophagy pathway in Drosophila autophagy related protein autophagy related protein 1 autophagy related protein 13 autophagy related protein 14 autophagy related protein 17 beclin 1 immunophilin phosphatidylinositol 3 phosphate protein serine threonine kinase VPS15 unclassified drug zonda autophagy related protein Drosophila protein immunophilin phosphatidylinositol 3 kinase phosphatidylinositol 3 phosphate polyphosphoinositide Article autophagosome autophagy carboxy terminal sequence controlled study Drosophila melanogaster endoplasmic reticulum gene expression gene function gene locus nonhuman organelle biogenesis priority journal protein localization protein protein interaction protein structure transmission electron microscopy animal autophagy Drosophila melanogaster genetics metabolism phagosome physiology signal transduction Animals Autophagy Autophagy-Related Proteins Class III Phosphatidylinositol 3-Kinases Drosophila melanogaster Drosophila Proteins Immunophilins Phagosomes Phosphatidylinositol 3-Kinases Phosphatidylinositol Phosphates Signal Transduction Autophagy is an evolutionary conserved process by which eukaryotic cells undergo self-digestion of cytoplasmic components. Here we report that a novel Drosophila immunophilin, which we have named Zonda, is critically required for starvation-induced autophagy. We show that Zonda operates at early stages of the process, specifically for Vps34-mediated phosphatidylinositol 3-phosphate (PI3P) deposition. Zonda displays an even distribution under basal conditions and, soon after starvation, nucleates in endoplasmic reticulum-associated foci that colocalize with omegasome markers. Zonda nucleation depends on Atg1, Atg13, and Atg17 but does not require Vps34, Vps15, Atg6, or Atg14. Zonda interacts physically with Atg1 through its kinase domain, as well as with Atg6 and Vps34. We propose that Zonda is an early component of the autophagy cascade necessary for Vps34-dependent PI3P deposition and omegasome formation. © 2017 Melani, Valko, Romero, et al. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10591524_v28_n22_p3070_Melani http://hdl.handle.net/20.500.12110/paper_10591524_v28_n22_p3070_Melani |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
autophagy related protein autophagy related protein 1 autophagy related protein 13 autophagy related protein 14 autophagy related protein 17 beclin 1 immunophilin phosphatidylinositol 3 phosphate protein serine threonine kinase VPS15 unclassified drug zonda autophagy related protein Drosophila protein immunophilin phosphatidylinositol 3 kinase phosphatidylinositol 3 phosphate polyphosphoinositide Article autophagosome autophagy carboxy terminal sequence controlled study Drosophila melanogaster endoplasmic reticulum gene expression gene function gene locus nonhuman organelle biogenesis priority journal protein localization protein protein interaction protein structure transmission electron microscopy animal autophagy Drosophila melanogaster genetics metabolism phagosome physiology signal transduction Animals Autophagy Autophagy-Related Proteins Class III Phosphatidylinositol 3-Kinases Drosophila melanogaster Drosophila Proteins Immunophilins Phagosomes Phosphatidylinositol 3-Kinases Phosphatidylinositol Phosphates Signal Transduction |
| spellingShingle |
autophagy related protein autophagy related protein 1 autophagy related protein 13 autophagy related protein 14 autophagy related protein 17 beclin 1 immunophilin phosphatidylinositol 3 phosphate protein serine threonine kinase VPS15 unclassified drug zonda autophagy related protein Drosophila protein immunophilin phosphatidylinositol 3 kinase phosphatidylinositol 3 phosphate polyphosphoinositide Article autophagosome autophagy carboxy terminal sequence controlled study Drosophila melanogaster endoplasmic reticulum gene expression gene function gene locus nonhuman organelle biogenesis priority journal protein localization protein protein interaction protein structure transmission electron microscopy animal autophagy Drosophila melanogaster genetics metabolism phagosome physiology signal transduction Animals Autophagy Autophagy-Related Proteins Class III Phosphatidylinositol 3-Kinases Drosophila melanogaster Drosophila Proteins Immunophilins Phagosomes Phosphatidylinositol 3-Kinases Phosphatidylinositol Phosphates Signal Transduction Zonda is a novel early component of the autophagy pathway in Drosophila |
| topic_facet |
autophagy related protein autophagy related protein 1 autophagy related protein 13 autophagy related protein 14 autophagy related protein 17 beclin 1 immunophilin phosphatidylinositol 3 phosphate protein serine threonine kinase VPS15 unclassified drug zonda autophagy related protein Drosophila protein immunophilin phosphatidylinositol 3 kinase phosphatidylinositol 3 phosphate polyphosphoinositide Article autophagosome autophagy carboxy terminal sequence controlled study Drosophila melanogaster endoplasmic reticulum gene expression gene function gene locus nonhuman organelle biogenesis priority journal protein localization protein protein interaction protein structure transmission electron microscopy animal autophagy Drosophila melanogaster genetics metabolism phagosome physiology signal transduction Animals Autophagy Autophagy-Related Proteins Class III Phosphatidylinositol 3-Kinases Drosophila melanogaster Drosophila Proteins Immunophilins Phagosomes Phosphatidylinositol 3-Kinases Phosphatidylinositol Phosphates Signal Transduction |
| description |
Autophagy is an evolutionary conserved process by which eukaryotic cells undergo self-digestion of cytoplasmic components. Here we report that a novel Drosophila immunophilin, which we have named Zonda, is critically required for starvation-induced autophagy. We show that Zonda operates at early stages of the process, specifically for Vps34-mediated phosphatidylinositol 3-phosphate (PI3P) deposition. Zonda displays an even distribution under basal conditions and, soon after starvation, nucleates in endoplasmic reticulum-associated foci that colocalize with omegasome markers. Zonda nucleation depends on Atg1, Atg13, and Atg17 but does not require Vps34, Vps15, Atg6, or Atg14. Zonda interacts physically with Atg1 through its kinase domain, as well as with Atg6 and Vps34. We propose that Zonda is an early component of the autophagy cascade necessary for Vps34-dependent PI3P deposition and omegasome formation. © 2017 Melani, Valko, Romero, et al. |
| title |
Zonda is a novel early component of the autophagy pathway in Drosophila |
| title_short |
Zonda is a novel early component of the autophagy pathway in Drosophila |
| title_full |
Zonda is a novel early component of the autophagy pathway in Drosophila |
| title_fullStr |
Zonda is a novel early component of the autophagy pathway in Drosophila |
| title_full_unstemmed |
Zonda is a novel early component of the autophagy pathway in Drosophila |
| title_sort |
zonda is a novel early component of the autophagy pathway in drosophila |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10591524_v28_n22_p3070_Melani http://hdl.handle.net/20.500.12110/paper_10591524_v28_n22_p3070_Melani |
| _version_ |
1768546686361141248 |