Mutations boost microbial PHA output

Researchers at Cuidad Universitaria-Pabellón 2 in Argentina investigated the effects of different mutations on producing poly 3-hydroxybutyrate (PHB) in recombinant Escherichia coli. The group focused on mutations in the arcA regulator in recombinant E. coli strains carrying PHA synthesis genes from...

Descripción completa

Detalles Bibliográficos
Autor principal: Méndez, Beatriz Silvia
Publicado: 2006
Materias:
Additives
Bioreactors
Escherichia coli
Genes
Strain
Synthesis (chemical)
Mutations
Polyhydroxyalkanoate (pha) plastics
Polyhydroxybutyrate (PHB)
Recombinant strains
Plastic products
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10567194_v28_n5_p9_Mendez
http://hdl.handle.net/20.500.12110/paper_10567194_v28_n5_p9_Mendez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_10567194_v28_n5_p9_Mendez
record_format dspace
spelling paper:paper_10567194_v28_n5_p9_Mendez2023-06-08T16:03:17Z Mutations boost microbial PHA output Méndez, Beatriz Silvia Additives Bioreactors Escherichia coli Genes Strain Synthesis (chemical) Mutations Polyhydroxyalkanoate (pha) plastics Polyhydroxybutyrate (PHB) Recombinant strains Plastic products Researchers at Cuidad Universitaria-Pabellón 2 in Argentina investigated the effects of different mutations on producing poly 3-hydroxybutyrate (PHB) in recombinant Escherichia coli. The group focused on mutations in the arcA regulator in recombinant E. coli strains carrying PHA synthesis genes from Azotobacter sp. strain FA8. The wildtype E. coli strain did not produce PHB under low-oxygen conditions in shaken flask cultures. However, recombinant strains carrying the mutants accumulated up to 24% of their cell dry weight when grown under the same conditions. Cultured in a bioreactor under microaerobic conditions the arcA deletion mutant achieved a PHB content of 27%. The arcA2 allele produced 35% PHB under the same conditions. The arc2A strain grew at a good growth rate on a simple medium. However, the deletion mutant needed several nutritional additives and the growth rate was still not as high as that of the arcA2 strain. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10567194_v28_n5_p9_Mendez http://hdl.handle.net/20.500.12110/paper_10567194_v28_n5_p9_Mendez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Additives
Bioreactors
Escherichia coli
Genes
Strain
Synthesis (chemical)
Mutations
Polyhydroxyalkanoate (pha) plastics
Polyhydroxybutyrate (PHB)
Recombinant strains
Plastic products
spellingShingle Additives
Bioreactors
Escherichia coli
Genes
Strain
Synthesis (chemical)
Mutations
Polyhydroxyalkanoate (pha) plastics
Polyhydroxybutyrate (PHB)
Recombinant strains
Plastic products
Méndez, Beatriz Silvia
Mutations boost microbial PHA output
topic_facet Additives
Bioreactors
Escherichia coli
Genes
Strain
Synthesis (chemical)
Mutations
Polyhydroxyalkanoate (pha) plastics
Polyhydroxybutyrate (PHB)
Recombinant strains
Plastic products
description Researchers at Cuidad Universitaria-Pabellón 2 in Argentina investigated the effects of different mutations on producing poly 3-hydroxybutyrate (PHB) in recombinant Escherichia coli. The group focused on mutations in the arcA regulator in recombinant E. coli strains carrying PHA synthesis genes from Azotobacter sp. strain FA8. The wildtype E. coli strain did not produce PHB under low-oxygen conditions in shaken flask cultures. However, recombinant strains carrying the mutants accumulated up to 24% of their cell dry weight when grown under the same conditions. Cultured in a bioreactor under microaerobic conditions the arcA deletion mutant achieved a PHB content of 27%. The arcA2 allele produced 35% PHB under the same conditions. The arc2A strain grew at a good growth rate on a simple medium. However, the deletion mutant needed several nutritional additives and the growth rate was still not as high as that of the arcA2 strain.
author Méndez, Beatriz Silvia
author_facet Méndez, Beatriz Silvia
author_sort Méndez, Beatriz Silvia
title Mutations boost microbial PHA output
title_short Mutations boost microbial PHA output
title_full Mutations boost microbial PHA output
title_fullStr Mutations boost microbial PHA output
title_full_unstemmed Mutations boost microbial PHA output
title_sort mutations boost microbial pha output
publishDate 2006
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10567194_v28_n5_p9_Mendez
http://hdl.handle.net/20.500.12110/paper_10567194_v28_n5_p9_Mendez
work_keys_str_mv AT mendezbeatrizsilvia mutationsboostmicrobialphaoutput
_version_ 1768546312729395200

Ejemplares similares