Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens

Bacillus amyloliquefaciens PGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars of Phaseolus vulgaris L. were evaluated: cv. Nag (black bean) and cv. A...

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Detalles Bibliográficos
Autor principal: Erra Balsells, Rosa
Publicado: 2017
Materias:
Bacillus amyloliquefaciens
Fusarium solani
Lipopeptides
Phaseolus vulgaris L
Sclerotinia sclerotiorum
Seed-protector
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10499644_v105_n_p93_Torres
http://hdl.handle.net/20.500.12110/paper_10499644_v105_n_p93_Torres
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_10499644_v105_n_p93_Torres
record_format dspace
spelling paper:paper_10499644_v105_n_p93_Torres2023-06-08T16:01:23Z Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens Erra Balsells, Rosa Bacillus amyloliquefaciens Fusarium solani Lipopeptides Phaseolus vulgaris L Sclerotinia sclerotiorum Seed-protector Bacillus amyloliquefaciens PGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars of Phaseolus vulgaris L. were evaluated: cv. Nag (black bean) and cv. Alubia (white bean). Aspergillus spp., Penicillium spp. and Fusarium spp. constituted the natural fungal biota of both seeds, whereas white bean and black bean also exhibited Cladosporium spp. and Rhizopus spp., respectively. B. amyloliquefaciens PGPBacCA1 prevented the development of the endophytic fungi of black bean, while only Cladosporium spp. survived in the white variety. Growth chamber assays were carried out and bacilli cells were applied on seeds without affecting neither the vigor nor the germination potential of either type of bean. In addition, B. amyloliquefaciens PGPBacCA1, by dual cultures, was able to inhibit the development of the following phytopathogenic fungi: Sclerotium rolfsii (35%), Sclerotinia sclerotiorum (76.5%), Rhizoctonia solani (73%), Fusarium solani (56.5%), and Penicillium spp. (71.5%). The UV-MALDI TOF MS analysis showed that B. amyloliquefaciens PGPBacCA1 co-produces different homologues of the lipopeptides surfactin, iturin and fengycin in the presence of S. sclerotiorum and F. solani. These compounds were identified as the main responsible for the antagonistic effect. SEM analysis confirmed the antifungal effects of the lipopeptides, which also caused damage to chlamydospores and sclerotia of Fusarium and Sclerotinia, respectively. B. amyloliquefaciens PGPBacCA1 can thus be applied to these bean seeds varieties as a potential bioprotection agent. © 2016 Elsevier Inc. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10499644_v105_n_p93_Torres http://hdl.handle.net/20.500.12110/paper_10499644_v105_n_p93_Torres
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bacillus amyloliquefaciens
Fusarium solani
Lipopeptides
Phaseolus vulgaris L
Sclerotinia sclerotiorum
Seed-protector
spellingShingle Bacillus amyloliquefaciens
Fusarium solani
Lipopeptides
Phaseolus vulgaris L
Sclerotinia sclerotiorum
Seed-protector
Erra Balsells, Rosa
Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
topic_facet Bacillus amyloliquefaciens
Fusarium solani
Lipopeptides
Phaseolus vulgaris L
Sclerotinia sclerotiorum
Seed-protector
description Bacillus amyloliquefaciens PGPBacCA1 was studied regarding its aptitude to protect common bean seeds from their intrinsic pathogens. Also, the inhibition of different environmental phytopathogenic fungi was tested. Two cultivars of Phaseolus vulgaris L. were evaluated: cv. Nag (black bean) and cv. Alubia (white bean). Aspergillus spp., Penicillium spp. and Fusarium spp. constituted the natural fungal biota of both seeds, whereas white bean and black bean also exhibited Cladosporium spp. and Rhizopus spp., respectively. B. amyloliquefaciens PGPBacCA1 prevented the development of the endophytic fungi of black bean, while only Cladosporium spp. survived in the white variety. Growth chamber assays were carried out and bacilli cells were applied on seeds without affecting neither the vigor nor the germination potential of either type of bean. In addition, B. amyloliquefaciens PGPBacCA1, by dual cultures, was able to inhibit the development of the following phytopathogenic fungi: Sclerotium rolfsii (35%), Sclerotinia sclerotiorum (76.5%), Rhizoctonia solani (73%), Fusarium solani (56.5%), and Penicillium spp. (71.5%). The UV-MALDI TOF MS analysis showed that B. amyloliquefaciens PGPBacCA1 co-produces different homologues of the lipopeptides surfactin, iturin and fengycin in the presence of S. sclerotiorum and F. solani. These compounds were identified as the main responsible for the antagonistic effect. SEM analysis confirmed the antifungal effects of the lipopeptides, which also caused damage to chlamydospores and sclerotia of Fusarium and Sclerotinia, respectively. B. amyloliquefaciens PGPBacCA1 can thus be applied to these bean seeds varieties as a potential bioprotection agent. © 2016 Elsevier Inc.
author Erra Balsells, Rosa
author_facet Erra Balsells, Rosa
author_sort Erra Balsells, Rosa
title Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
title_short Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
title_full Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
title_fullStr Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
title_full_unstemmed Biological activity of the lipopeptide-producing Bacillus amyloliquefaciens PGPBacCA1 on common bean Phaseolus vulgaris L. pathogens
title_sort biological activity of the lipopeptide-producing bacillus amyloliquefaciens pgpbacca1 on common bean phaseolus vulgaris l. pathogens
publishDate 2017
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10499644_v105_n_p93_Torres
http://hdl.handle.net/20.500.12110/paper_10499644_v105_n_p93_Torres
work_keys_str_mv AT errabalsellsrosa biologicalactivityofthelipopeptideproducingbacillusamyloliquefacienspgpbacca1oncommonbeanphaseolusvulgarislpathogens
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