Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients
Problem: Women with celiac disease (CD) are often affected by atypical presentations of the disease associated with reproductive disorders as a main extra-digestive complaint. Here, we analyzed if autoantibodies against tissue transglutaminase (tTG) in sera from CD patients with reproductive disorde...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10467408_v72_n5_p485_Sonora http://hdl.handle.net/20.500.12110/paper_10467408_v72_n5_p485_Sonora |
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paper:paper_10467408_v72_n5_p485_Sonora2023-06-08T16:01:15Z Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients Celiac patients Pregnancy complications Tissue transglutaminase lactadherin protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase antibody autoantibody guanine nucleotide binding protein protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase 2 adult antibody blood level apoptosis Article celiac disease cell migration cell proliferation clearance clinical article controlled study cytotrophoblast female human human cell human tissue phagocyte pregnancy complication priority journal protein binding protein expression protein interaction tissue injury trophoblast Western blotting wound healing blood celiac disease cell line enzymology immunology metabolism middle aged pregnancy pregnancy complication trophoblast Adult Autoantibodies Celiac Disease Cell Line Female GTP-Binding Proteins Humans Middle Aged Pregnancy Pregnancy Complications Transglutaminases Trophoblasts Problem: Women with celiac disease (CD) are often affected by atypical presentations of the disease associated with reproductive disorders as a main extra-digestive complaint. Here, we analyzed if autoantibodies against tissue transglutaminase (tTG) in sera from CD patients with reproductive disorders could display direct effects through their interaction with tTG expressed on trophoblast cells and phagocytes inducing tissue damage and interfering in the clearance of trophoblast apoptotic bodies. Method of study: Sera from CD women with reproductive disorders were obtained, and their ability to induce apoptosis of Swan-71 (cytotrophoblast cell line) and to modulate the wound-healing and phagocytes process was tested. Results: Swan-71 cells expressed tTG and CD sera displayed a significant decrease in trophoblast cell migration and a delay in injury healing on trophoblast cells, compared with those observed with control sera. Moreover, CD sera significantly reduced trophoblast cell proliferation and increased apoptosis levels in comparison with those observed in the control sera. Finally, autoantibodies against tTG interfere in the clearance of trophoblast apoptotic bodies through a mechanism involving MFG-E8 (milk fat globulin-EGF factor 8)-tTG binding. Conclusion: The anti-tTG antibodies might contribute to trophoblast damage and disrupt the phagocytosis process of apoptotic bodies that could promote a pro-inflammatory microenvironment. © 2014 John Wiley & Sons A/S. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10467408_v72_n5_p485_Sonora http://hdl.handle.net/20.500.12110/paper_10467408_v72_n5_p485_Sonora |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Celiac patients Pregnancy complications Tissue transglutaminase lactadherin protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase antibody autoantibody guanine nucleotide binding protein protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase 2 adult antibody blood level apoptosis Article celiac disease cell migration cell proliferation clearance clinical article controlled study cytotrophoblast female human human cell human tissue phagocyte pregnancy complication priority journal protein binding protein expression protein interaction tissue injury trophoblast Western blotting wound healing blood celiac disease cell line enzymology immunology metabolism middle aged pregnancy pregnancy complication trophoblast Adult Autoantibodies Celiac Disease Cell Line Female GTP-Binding Proteins Humans Middle Aged Pregnancy Pregnancy Complications Transglutaminases Trophoblasts |
| spellingShingle |
Celiac patients Pregnancy complications Tissue transglutaminase lactadherin protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase antibody autoantibody guanine nucleotide binding protein protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase 2 adult antibody blood level apoptosis Article celiac disease cell migration cell proliferation clearance clinical article controlled study cytotrophoblast female human human cell human tissue phagocyte pregnancy complication priority journal protein binding protein expression protein interaction tissue injury trophoblast Western blotting wound healing blood celiac disease cell line enzymology immunology metabolism middle aged pregnancy pregnancy complication trophoblast Adult Autoantibodies Celiac Disease Cell Line Female GTP-Binding Proteins Humans Middle Aged Pregnancy Pregnancy Complications Transglutaminases Trophoblasts Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients |
| topic_facet |
Celiac patients Pregnancy complications Tissue transglutaminase lactadherin protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase antibody autoantibody guanine nucleotide binding protein protein glutamine gamma glutamyltransferase protein glutamine gamma glutamyltransferase 2 adult antibody blood level apoptosis Article celiac disease cell migration cell proliferation clearance clinical article controlled study cytotrophoblast female human human cell human tissue phagocyte pregnancy complication priority journal protein binding protein expression protein interaction tissue injury trophoblast Western blotting wound healing blood celiac disease cell line enzymology immunology metabolism middle aged pregnancy pregnancy complication trophoblast Adult Autoantibodies Celiac Disease Cell Line Female GTP-Binding Proteins Humans Middle Aged Pregnancy Pregnancy Complications Transglutaminases Trophoblasts |
| description |
Problem: Women with celiac disease (CD) are often affected by atypical presentations of the disease associated with reproductive disorders as a main extra-digestive complaint. Here, we analyzed if autoantibodies against tissue transglutaminase (tTG) in sera from CD patients with reproductive disorders could display direct effects through their interaction with tTG expressed on trophoblast cells and phagocytes inducing tissue damage and interfering in the clearance of trophoblast apoptotic bodies. Method of study: Sera from CD women with reproductive disorders were obtained, and their ability to induce apoptosis of Swan-71 (cytotrophoblast cell line) and to modulate the wound-healing and phagocytes process was tested. Results: Swan-71 cells expressed tTG and CD sera displayed a significant decrease in trophoblast cell migration and a delay in injury healing on trophoblast cells, compared with those observed with control sera. Moreover, CD sera significantly reduced trophoblast cell proliferation and increased apoptosis levels in comparison with those observed in the control sera. Finally, autoantibodies against tTG interfere in the clearance of trophoblast apoptotic bodies through a mechanism involving MFG-E8 (milk fat globulin-EGF factor 8)-tTG binding. Conclusion: The anti-tTG antibodies might contribute to trophoblast damage and disrupt the phagocytosis process of apoptotic bodies that could promote a pro-inflammatory microenvironment. © 2014 John Wiley & Sons A/S. |
| title |
Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients |
| title_short |
Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients |
| title_full |
Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients |
| title_fullStr |
Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients |
| title_full_unstemmed |
Tissue Transglutaminase on Trophoblast Cells as a Possible Target of Autoantibodies Contributing to Pregnancy Complications in Celiac Patients |
| title_sort |
tissue transglutaminase on trophoblast cells as a possible target of autoantibodies contributing to pregnancy complications in celiac patients |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10467408_v72_n5_p485_Sonora http://hdl.handle.net/20.500.12110/paper_10467408_v72_n5_p485_Sonora |
| _version_ |
1768544695510630400 |