Vasoactive Intestinal Peptide Induces an Immunosuppressant Microenvironment in the Maternal-Fetal Interface of Non-Obese Diabetic Mice and Improves Early Pregnancy Outcome

Problem: Impaired pregnancy in non-obese diabetic (NOD) mice was related to limited vascular remodeling and autoimmune background. Vasoactive intestinal peptide (VIP) has anti-inflammatory and immunosuppressant effects, so we explored its ability to modulate the immune microenvironment at the early...

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Autores principales:	Hauk, Vanesa Cintia, Franchi, Ana María, Ramhorst, Rosanna Elizabeth, Pérez Leirós, Claudia
Publicado:	2014
Materias:	Early pregnancy Foxp3 IL-10 non-obese diabetic mice TGF-β Vasoactive intestinal peptide interleukin 10 interleukin 17 retinoid related orphan receptor gamma transcription factor FOXP3 transforming growth factor beta vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 cytokine forkhead transcription factor FOXP3 protein, human animal experiment animal model animal tissue article controlled study female first trimester pregnancy gestational age giant cell immunoblotting immunohistochemistry immunomodulation in vitro study insulin dependent diabetes mellitus microenvironment mother fetus relationship mouse nidation nonhuman nonobese diabetic mouse pregnancy outcome priority journal protein expression reverse transcription polymerase chain reaction trophoblast animal cell culture diabetes mellitus disease model fetomaternal transfusion genetics human immunological tolerance immunology metabolism organ culture technique pathology pregnancy pregnancy complication tumor microenvironment uterus Animals Cells, Cultured Cellular Microenvironment Cytokines Diabetes Mellitus Disease Models, Animal Female Forkhead Transcription Factors Gestational Age Humans Immune Tolerance Maternal-Fetal Exchange Mice Mice, Inbred NOD Nuclear Receptor Subfamily 1, Group F, Member 3 Organ Culture Techniques Pregnancy Pregnancy Complications Pregnancy Outcome Receptors, Vasoactive Intestinal Peptide, Type II Uterus Vasoactive Intestinal Peptide
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10467408_v71_n2_p120_Hauk http://hdl.handle.net/20.500.12110/paper_10467408_v71_n2_p120_Hauk
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_10467408_v71_n2_p120_Hauk2023-06-08T16:01:15Z Vasoactive Intestinal Peptide Induces an Immunosuppressant Microenvironment in the Maternal-Fetal Interface of Non-Obese Diabetic Mice and Improves Early Pregnancy Outcome Hauk, Vanesa Cintia Franchi, Ana María Ramhorst, Rosanna Elizabeth Pérez Leirós, Claudia Early pregnancy Foxp3 IL-10 non-obese diabetic mice TGF-β Vasoactive intestinal peptide interleukin 10 interleukin 17 retinoid related orphan receptor gamma transcription factor FOXP3 transforming growth factor beta vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 cytokine forkhead transcription factor FOXP3 protein, human vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 2 animal experiment animal model animal tissue article controlled study female first trimester pregnancy gestational age giant cell immunoblotting immunohistochemistry immunomodulation in vitro study insulin dependent diabetes mellitus microenvironment mother fetus relationship mouse nidation nonhuman nonobese diabetic mouse pregnancy outcome priority journal protein expression reverse transcription polymerase chain reaction trophoblast animal cell culture diabetes mellitus disease model fetomaternal transfusion genetics human immunological tolerance immunology metabolism organ culture technique pathology pregnancy pregnancy complication pregnancy outcome tumor microenvironment uterus Animals Cells, Cultured Cellular Microenvironment Cytokines Diabetes Mellitus Disease Models, Animal Female Forkhead Transcription Factors Gestational Age Humans Immune Tolerance Maternal-Fetal Exchange Mice Mice, Inbred NOD Nuclear Receptor Subfamily 1, Group F, Member 3 Organ Culture Techniques Pregnancy Pregnancy Complications Pregnancy Outcome Receptors, Vasoactive Intestinal Peptide, Type II Uterus Vasoactive Intestinal Peptide Problem: Impaired pregnancy in non-obese diabetic (NOD) mice was related to limited vascular remodeling and autoimmune background. Vasoactive intestinal peptide (VIP) has anti-inflammatory and immunosuppressant effects, so we explored its ability to modulate the immune microenvironment at the early maternal-placental interface and improve pregnancy in NOD mice. Method of study: Implantation sites were isolated from pregnant NOD mice at gestational day 9.5 and were incubated with VIP for evaluation of cytokine or transcription factor expression by RT-PCR, immunoblotting, and immunohistochemistry. Alternatively, pregnant mice were injected with VIP at day 6.5 and studied at day 9.5. Results: VIP and VPAC receptors were detected in viable implantation sites. VIP immunostaining was found predominantly on trophoblast giant cells. The in vitro treatment of viable implantation sites with VIP increased IL-10, TGF-β, and Foxp3 expression. Sites with resorption processes presented lower VIP expression, reduced suppressant markers, and increased IL-17 and RORγT expression compared with viable sites and VIP reduced RORγT expression. Pregnant mice treated with VIP at day 6.5 presented an even distribution of viable implantation sites with an increased expression of IL-10, TGF- β, and Foxp3. Conclusion: VIP induces an immunosuppressant profile at the early maternal-placental interface of NOD mice and improves pregnancy outcome. © 2013 John Wiley & Sons Ltd. Fil:Hauk, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Franchi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramhorst, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Leirós, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10467408_v71_n2_p120_Hauk http://hdl.handle.net/20.500.12110/paper_10467408_v71_n2_p120_Hauk
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Early pregnancy Foxp3 IL-10 non-obese diabetic mice TGF-β Vasoactive intestinal peptide interleukin 10 interleukin 17 retinoid related orphan receptor gamma transcription factor FOXP3 transforming growth factor beta vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 cytokine forkhead transcription factor FOXP3 protein, human vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 2 animal experiment animal model animal tissue article controlled study female first trimester pregnancy gestational age giant cell immunoblotting immunohistochemistry immunomodulation in vitro study insulin dependent diabetes mellitus microenvironment mother fetus relationship mouse nidation nonhuman nonobese diabetic mouse pregnancy outcome priority journal protein expression reverse transcription polymerase chain reaction trophoblast animal cell culture diabetes mellitus disease model fetomaternal transfusion genetics human immunological tolerance immunology metabolism organ culture technique pathology pregnancy pregnancy complication pregnancy outcome tumor microenvironment uterus Animals Cells, Cultured Cellular Microenvironment Cytokines Diabetes Mellitus Disease Models, Animal Female Forkhead Transcription Factors Gestational Age Humans Immune Tolerance Maternal-Fetal Exchange Mice Mice, Inbred NOD Nuclear Receptor Subfamily 1, Group F, Member 3 Organ Culture Techniques Pregnancy Pregnancy Complications Pregnancy Outcome Receptors, Vasoactive Intestinal Peptide, Type II Uterus Vasoactive Intestinal Peptide
spellingShingle	Early pregnancy Foxp3 IL-10 non-obese diabetic mice TGF-β Vasoactive intestinal peptide interleukin 10 interleukin 17 retinoid related orphan receptor gamma transcription factor FOXP3 transforming growth factor beta vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 cytokine forkhead transcription factor FOXP3 protein, human vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 2 animal experiment animal model animal tissue article controlled study female first trimester pregnancy gestational age giant cell immunoblotting immunohistochemistry immunomodulation in vitro study insulin dependent diabetes mellitus microenvironment mother fetus relationship mouse nidation nonhuman nonobese diabetic mouse pregnancy outcome priority journal protein expression reverse transcription polymerase chain reaction trophoblast animal cell culture diabetes mellitus disease model fetomaternal transfusion genetics human immunological tolerance immunology metabolism organ culture technique pathology pregnancy pregnancy complication pregnancy outcome tumor microenvironment uterus Animals Cells, Cultured Cellular Microenvironment Cytokines Diabetes Mellitus Disease Models, Animal Female Forkhead Transcription Factors Gestational Age Humans Immune Tolerance Maternal-Fetal Exchange Mice Mice, Inbred NOD Nuclear Receptor Subfamily 1, Group F, Member 3 Organ Culture Techniques Pregnancy Pregnancy Complications Pregnancy Outcome Receptors, Vasoactive Intestinal Peptide, Type II Uterus Vasoactive Intestinal Peptide Hauk, Vanesa Cintia Franchi, Ana María Ramhorst, Rosanna Elizabeth Pérez Leirós, Claudia Vasoactive Intestinal Peptide Induces an Immunosuppressant Microenvironment in the Maternal-Fetal Interface of Non-Obese Diabetic Mice and Improves Early Pregnancy Outcome
topic_facet	Early pregnancy Foxp3 IL-10 non-obese diabetic mice TGF-β Vasoactive intestinal peptide interleukin 10 interleukin 17 retinoid related orphan receptor gamma transcription factor FOXP3 transforming growth factor beta vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 1 vasoactive intestinal polypeptide receptor 2 cytokine forkhead transcription factor FOXP3 protein, human vasoactive intestinal polypeptide vasoactive intestinal polypeptide receptor 2 animal experiment animal model animal tissue article controlled study female first trimester pregnancy gestational age giant cell immunoblotting immunohistochemistry immunomodulation in vitro study insulin dependent diabetes mellitus microenvironment mother fetus relationship mouse nidation nonhuman nonobese diabetic mouse pregnancy outcome priority journal protein expression reverse transcription polymerase chain reaction trophoblast animal cell culture diabetes mellitus disease model fetomaternal transfusion genetics human immunological tolerance immunology metabolism organ culture technique pathology pregnancy pregnancy complication pregnancy outcome tumor microenvironment uterus Animals Cells, Cultured Cellular Microenvironment Cytokines Diabetes Mellitus Disease Models, Animal Female Forkhead Transcription Factors Gestational Age Humans Immune Tolerance Maternal-Fetal Exchange Mice Mice, Inbred NOD Nuclear Receptor Subfamily 1, Group F, Member 3 Organ Culture Techniques Pregnancy Pregnancy Complications Pregnancy Outcome Receptors, Vasoactive Intestinal Peptide, Type II Uterus Vasoactive Intestinal Peptide
description	Problem: Impaired pregnancy in non-obese diabetic (NOD) mice was related to limited vascular remodeling and autoimmune background. Vasoactive intestinal peptide (VIP) has anti-inflammatory and immunosuppressant effects, so we explored its ability to modulate the immune microenvironment at the early maternal-placental interface and improve pregnancy in NOD mice. Method of study: Implantation sites were isolated from pregnant NOD mice at gestational day 9.5 and were incubated with VIP for evaluation of cytokine or transcription factor expression by RT-PCR, immunoblotting, and immunohistochemistry. Alternatively, pregnant mice were injected with VIP at day 6.5 and studied at day 9.5. Results: VIP and VPAC receptors were detected in viable implantation sites. VIP immunostaining was found predominantly on trophoblast giant cells. The in vitro treatment of viable implantation sites with VIP increased IL-10, TGF-β, and Foxp3 expression. Sites with resorption processes presented lower VIP expression, reduced suppressant markers, and increased IL-17 and RORγT expression compared with viable sites and VIP reduced RORγT expression. Pregnant mice treated with VIP at day 6.5 presented an even distribution of viable implantation sites with an increased expression of IL-10, TGF- β, and Foxp3. Conclusion: VIP induces an immunosuppressant profile at the early maternal-placental interface of NOD mice and improves pregnancy outcome. © 2013 John Wiley & Sons Ltd.
author	Hauk, Vanesa Cintia Franchi, Ana María Ramhorst, Rosanna Elizabeth Pérez Leirós, Claudia
author_facet	Hauk, Vanesa Cintia Franchi, Ana María Ramhorst, Rosanna Elizabeth Pérez Leirós, Claudia
author_sort	Hauk, Vanesa Cintia
title	Vasoactive Intestinal Peptide Induces an Immunosuppressant Microenvironment in the Maternal-Fetal Interface of Non-Obese Diabetic Mice and Improves Early Pregnancy Outcome
title_short	Vasoactive Intestinal Peptide Induces an Immunosuppressant Microenvironment in the Maternal-Fetal Interface of Non-Obese Diabetic Mice and Improves Early Pregnancy Outcome
title_full	Vasoactive Intestinal Peptide Induces an Immunosuppressant Microenvironment in the Maternal-Fetal Interface of Non-Obese Diabetic Mice and Improves Early Pregnancy Outcome
title_fullStr	Vasoactive Intestinal Peptide Induces an Immunosuppressant Microenvironment in the Maternal-Fetal Interface of Non-Obese Diabetic Mice and Improves Early Pregnancy Outcome
title_full_unstemmed	Vasoactive Intestinal Peptide Induces an Immunosuppressant Microenvironment in the Maternal-Fetal Interface of Non-Obese Diabetic Mice and Improves Early Pregnancy Outcome
title_sort	vasoactive intestinal peptide induces an immunosuppressant microenvironment in the maternal-fetal interface of non-obese diabetic mice and improves early pregnancy outcome
publishDate	2014
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10467408_v71_n2_p120_Hauk http://hdl.handle.net/20.500.12110/paper_10467408_v71_n2_p120_Hauk
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Vasoactive Intestinal Peptide Induces an Immunosuppressant Microenvironment in the Maternal-Fetal Interface of Non-Obese Diabetic Mice and Improves Early Pregnancy Outcome

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