Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration

Problem Establishment of a successful pregnancy relies on a complex fetal-mother communication that starts with the embryo adhering and invading the endometrium. This requires remodeling of extracellular matrix, performed by metalloproteinases. Cytokines, such as interferon-γ (IFN-γ), play a role in...

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Autores principales:	Fontana, Vanina Andrea, Cebral, Elisa, Calvo, Juan Carlos
Publicado:	2010
Materias:	Cytokines Extracellular matrix Placenta gamma interferon gelatinase gelatinase A gelatinase B leptin metalloproteinase article cell culture cell migration cell strain JEG 3 controlled study cytotrophoblast enzyme activity female human human cell human cell culture pregnancy priority journal protein synthesis Western blotting zymography animal biosynthesis cell motion drug effect enzymology metabolism mouse physiology polyacrylamide gel electrophoresis trophoblast tumor cell line Animals Blotting, Western Cell Line, Tumor Cell Movement Electrophoresis, Polyacrylamide Gel Female Humans Interferon-gamma Leptin Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Mice Trophoblasts
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10467408_v64_n1_p20_Fontana http://hdl.handle.net/20.500.12110/paper_10467408_v64_n1_p20_Fontana
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10467408_v64_n1_p20_Fontana
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spelling	paper:paper_10467408_v64_n1_p20_Fontana2023-06-08T16:01:14Z Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration Fontana, Vanina Andrea Cebral, Elisa Calvo, Juan Carlos Cytokines Extracellular matrix Placenta gamma interferon gelatinase gelatinase A gelatinase B leptin metalloproteinase gamma interferon gelatinase A gelatinase B leptin article cell culture cell migration cell strain JEG 3 controlled study cytotrophoblast enzyme activity female human human cell human cell culture pregnancy priority journal protein synthesis Western blotting zymography animal biosynthesis cell motion drug effect enzymology metabolism mouse physiology polyacrylamide gel electrophoresis trophoblast tumor cell line Animals Blotting, Western Cell Line, Tumor Cell Movement Electrophoresis, Polyacrylamide Gel Female Humans Interferon-gamma Leptin Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Mice Trophoblasts Problem Establishment of a successful pregnancy relies on a complex fetal-mother communication that starts with the embryo adhering and invading the endometrium. This requires remodeling of extracellular matrix, performed by metalloproteinases. Cytokines, such as interferon-γ (IFN-γ), play a role in implantation and could affect the success of pregnancy.Method of study Using JEG-3 cell line as model, we cultured the cells in the presence or absence of IFN-γ and determined the activities of MMP-2 and MMP-9 using zymography and the secretion of leptin using Western blot.Results Interferon-γ inhibits gelatinase activity from MMP-2 and MMP-9 in a dose-dependent manner, reducing the secretion of leptin (not because of a general inhibition on protein synthesis) and impairs cell migration on Matrigel.Conclusion Our results correlate with previous reports from our laboratory indicating that IFN- γ is deleterious for mouse embryo outgrowth, having an effect on metalloproteinases activity as well as leptin secretion. © 2010 John Wiley & Sons A/S. Fil:Fontana, V.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10467408_v64_n1_p20_Fontana http://hdl.handle.net/20.500.12110/paper_10467408_v64_n1_p20_Fontana
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cytokines Extracellular matrix Placenta gamma interferon gelatinase gelatinase A gelatinase B leptin metalloproteinase gamma interferon gelatinase A gelatinase B leptin article cell culture cell migration cell strain JEG 3 controlled study cytotrophoblast enzyme activity female human human cell human cell culture pregnancy priority journal protein synthesis Western blotting zymography animal biosynthesis cell motion drug effect enzymology metabolism mouse physiology polyacrylamide gel electrophoresis trophoblast tumor cell line Animals Blotting, Western Cell Line, Tumor Cell Movement Electrophoresis, Polyacrylamide Gel Female Humans Interferon-gamma Leptin Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Mice Trophoblasts
spellingShingle	Cytokines Extracellular matrix Placenta gamma interferon gelatinase gelatinase A gelatinase B leptin metalloproteinase gamma interferon gelatinase A gelatinase B leptin article cell culture cell migration cell strain JEG 3 controlled study cytotrophoblast enzyme activity female human human cell human cell culture pregnancy priority journal protein synthesis Western blotting zymography animal biosynthesis cell motion drug effect enzymology metabolism mouse physiology polyacrylamide gel electrophoresis trophoblast tumor cell line Animals Blotting, Western Cell Line, Tumor Cell Movement Electrophoresis, Polyacrylamide Gel Female Humans Interferon-gamma Leptin Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Mice Trophoblasts Fontana, Vanina Andrea Cebral, Elisa Calvo, Juan Carlos Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration
topic_facet	Cytokines Extracellular matrix Placenta gamma interferon gelatinase gelatinase A gelatinase B leptin metalloproteinase gamma interferon gelatinase A gelatinase B leptin article cell culture cell migration cell strain JEG 3 controlled study cytotrophoblast enzyme activity female human human cell human cell culture pregnancy priority journal protein synthesis Western blotting zymography animal biosynthesis cell motion drug effect enzymology metabolism mouse physiology polyacrylamide gel electrophoresis trophoblast tumor cell line Animals Blotting, Western Cell Line, Tumor Cell Movement Electrophoresis, Polyacrylamide Gel Female Humans Interferon-gamma Leptin Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Mice Trophoblasts
description	Problem Establishment of a successful pregnancy relies on a complex fetal-mother communication that starts with the embryo adhering and invading the endometrium. This requires remodeling of extracellular matrix, performed by metalloproteinases. Cytokines, such as interferon-γ (IFN-γ), play a role in implantation and could affect the success of pregnancy.Method of study Using JEG-3 cell line as model, we cultured the cells in the presence or absence of IFN-γ and determined the activities of MMP-2 and MMP-9 using zymography and the secretion of leptin using Western blot.Results Interferon-γ inhibits gelatinase activity from MMP-2 and MMP-9 in a dose-dependent manner, reducing the secretion of leptin (not because of a general inhibition on protein synthesis) and impairs cell migration on Matrigel.Conclusion Our results correlate with previous reports from our laboratory indicating that IFN- γ is deleterious for mouse embryo outgrowth, having an effect on metalloproteinases activity as well as leptin secretion. © 2010 John Wiley & Sons A/S.
author	Fontana, Vanina Andrea Cebral, Elisa Calvo, Juan Carlos
author_facet	Fontana, Vanina Andrea Cebral, Elisa Calvo, Juan Carlos
author_sort	Fontana, Vanina Andrea
title	Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration
title_short	Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration
title_full	Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration
title_fullStr	Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration
title_full_unstemmed	Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration
title_sort	interferon-γ inhibits metalloproteinase activity and cytotrophoblast cell migration
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10467408_v64_n1_p20_Fontana http://hdl.handle.net/20.500.12110/paper_10467408_v64_n1_p20_Fontana
work_keys_str_mv	AT fontanavaninaandrea interferonginhibitsmetalloproteinaseactivityandcytotrophoblastcellmigration AT cebralelisa interferonginhibitsmetalloproteinaseactivityandcytotrophoblastcellmigration AT calvojuancarlos interferonginhibitsmetalloproteinaseactivityandcytotrophoblastcellmigration
_version_	1768546311765753856

Interferon-γ Inhibits Metalloproteinase Activity and Cytotrophoblast Cell Migration

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