Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2
We screened for pollen-specific kinase genes, which are potential signal transduction components of pollen-pistil interactions, and isolated two structurally related receptor-like kinases (RLKs) from tomato, LePRK1 and LePRK2. These kinases are similar to a pollen-expressed RLK from petunia, but the...
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1998
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10404651_v10_n3_p319_Muschietti http://hdl.handle.net/20.500.12110/paper_10404651_v10_n3_p319_Muschietti |
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paper:paper_10404651_v10_n3_p319_Muschietti2023-06-08T16:00:47Z Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2 membrane protein protein kinase recombinant protein amino acid sequence article cell wall enzymology Escherichia coli gene gene expression genetics germination in vitro study metabolism molecular genetics nucleotide sequence phosphorylation physiology plant pollen sequence alignment tomato Amino Acid Sequence Cell Wall Escherichia coli Gene Expression Genes, Plant Germination Lycopersicon esculentum Membrane Proteins Molecular Sequence Data Phosphorylation Plant Components Pollen Protein Kinases Recombinant Proteins Sequence Alignment Escherichia coli Lycopersicon esculentum Petunia We screened for pollen-specific kinase genes, which are potential signal transduction components of pollen-pistil interactions, and isolated two structurally related receptor-like kinases (RLKs) from tomato, LePRK1 and LePRK2. These kinases are similar to a pollen-expressed RLK from petunia, but they are expressed later during pollen development than is the petunia RLK. The abundance of LePRK2 increases when pollen germinates, but LePRK1 remains constant. Both LePRK1 and LePRK2 are localized to the plasma membrane/cell wall of growing pollen tubes. Both kinase domains have kinase activity when expressed in Escherichia coli. In phosphorylation assays with pollen membrane preparations, LePRK2, but not LePRK1, is phosphorylated, and the addition of tomato style, but not leaf, extracts to these membrane preparations results at least partially in specific dephosphorylation of LePRK2. Taken together, these results suggest that LePRK1 and LePRK2 play different roles in postpollination events and that at least LePRK2 may mediate some pistil response. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10404651_v10_n3_p319_Muschietti http://hdl.handle.net/20.500.12110/paper_10404651_v10_n3_p319_Muschietti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
membrane protein protein kinase recombinant protein amino acid sequence article cell wall enzymology Escherichia coli gene gene expression genetics germination in vitro study metabolism molecular genetics nucleotide sequence phosphorylation physiology plant pollen sequence alignment tomato Amino Acid Sequence Cell Wall Escherichia coli Gene Expression Genes, Plant Germination Lycopersicon esculentum Membrane Proteins Molecular Sequence Data Phosphorylation Plant Components Pollen Protein Kinases Recombinant Proteins Sequence Alignment Escherichia coli Lycopersicon esculentum Petunia |
| spellingShingle |
membrane protein protein kinase recombinant protein amino acid sequence article cell wall enzymology Escherichia coli gene gene expression genetics germination in vitro study metabolism molecular genetics nucleotide sequence phosphorylation physiology plant pollen sequence alignment tomato Amino Acid Sequence Cell Wall Escherichia coli Gene Expression Genes, Plant Germination Lycopersicon esculentum Membrane Proteins Molecular Sequence Data Phosphorylation Plant Components Pollen Protein Kinases Recombinant Proteins Sequence Alignment Escherichia coli Lycopersicon esculentum Petunia Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2 |
| topic_facet |
membrane protein protein kinase recombinant protein amino acid sequence article cell wall enzymology Escherichia coli gene gene expression genetics germination in vitro study metabolism molecular genetics nucleotide sequence phosphorylation physiology plant pollen sequence alignment tomato Amino Acid Sequence Cell Wall Escherichia coli Gene Expression Genes, Plant Germination Lycopersicon esculentum Membrane Proteins Molecular Sequence Data Phosphorylation Plant Components Pollen Protein Kinases Recombinant Proteins Sequence Alignment Escherichia coli Lycopersicon esculentum Petunia |
| description |
We screened for pollen-specific kinase genes, which are potential signal transduction components of pollen-pistil interactions, and isolated two structurally related receptor-like kinases (RLKs) from tomato, LePRK1 and LePRK2. These kinases are similar to a pollen-expressed RLK from petunia, but they are expressed later during pollen development than is the petunia RLK. The abundance of LePRK2 increases when pollen germinates, but LePRK1 remains constant. Both LePRK1 and LePRK2 are localized to the plasma membrane/cell wall of growing pollen tubes. Both kinase domains have kinase activity when expressed in Escherichia coli. In phosphorylation assays with pollen membrane preparations, LePRK2, but not LePRK1, is phosphorylated, and the addition of tomato style, but not leaf, extracts to these membrane preparations results at least partially in specific dephosphorylation of LePRK2. Taken together, these results suggest that LePRK1 and LePRK2 play different roles in postpollination events and that at least LePRK2 may mediate some pistil response. |
| title |
Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2 |
| title_short |
Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2 |
| title_full |
Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2 |
| title_fullStr |
Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2 |
| title_full_unstemmed |
Pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases LePRK1 and LePRK2 |
| title_sort |
pollen tube localization implies a role in pollen-pistil interactions for the tomato receptor-like protein kinases leprk1 and leprk2 |
| publishDate |
1998 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10404651_v10_n3_p319_Muschietti http://hdl.handle.net/20.500.12110/paper_10404651_v10_n3_p319_Muschietti |
| _version_ |
1768543045468291072 |