Tankyrase inhibition regulates corpus luteum development and luteal function in gonadotropin-treated rats
Tankyrases are physiological regulators of Axin, a protein involved in several cellular processes, including Wnt signaling. Here, we investigated the effect of a specific Tankyrase inhibitor (XAV939) in follicular-luteal dynamics, and its possible relationship with ovarian vascular development. Stud...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v84_n8_p719_Accialini http://hdl.handle.net/20.500.12110/paper_1040452X_v84_n8_p719_Accialini |
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paper:paper_1040452X_v84_n8_p719_Accialini2023-06-08T16:00:47Z Tankyrase inhibition regulates corpus luteum development and luteal function in gonadotropin-treated rats luteinization progesterone steroidogenesis Tankyrase inhibitor Wnt/β-catenin 7,8 dihydro 2 [4 (trifluoromethyl)phenyl] 5h thiopyrano[4,3 d]pyrimidin 4 ol beta catenin gonadotropin mitogen activated protein kinase progesterone protein kinase B steroidogenic acute regulatory protein tankyrase vasculotropin Wnt protein beta catenin gonadotropin progesterone tankyrase Wnt protein angiogenesis animal experiment animal tissue antral follicle apoptosis Article cell proliferation controlled study corpus luteum enzyme inhibition enzyme phosphorylation female in vivo study luteal cell nonhuman ovary follicle development priority journal progesterone blood level rat steroidogenesis Wnt signaling pathway animal antagonists and inhibitors corpus luteum metabolism physiology signal transduction Animals beta Catenin Corpus Luteum Female Gonadotropins Progesterone Rats Signal Transduction Tankyrases Wnt Proteins Tankyrases are physiological regulators of Axin, a protein involved in several cellular processes, including Wnt signaling. Here, we investigated the effect of a specific Tankyrase inhibitor (XAV939) in follicular-luteal dynamics, and its possible relationship with ovarian vascular development. Studies were designed to analyze the effect of intrabursa administration of XAV939 in gonadotropin-treated prepubertal rats. In particular, we examined follicle and corpus luteum development, steroidogenesis, angiogenic markers, and apoptotic parameters. We found that in vivo inhibition of Wnt signaling impaired corpus luteum development, with a decrease in the number of corpora lutea balanced by a high number of cysts; decreased circulating progesterone levels, likely due to a decrease in Steroidogenic acute regulatory protein content in the corpus luteum; and increased pro-apoptotic parameters. In addition, Extracellular signal-regulated kinase phosphorylation, Vascular endothelium growth factor 120 content, and endothelial cell area were diminished in corpora lutea of inhibitor-treated ovaries. Thus, Wnt/β-catenin signaling appears to participate in the regulation of corpus luteum development and luteal cell function. © 2017 Wiley Periodicals, Inc. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v84_n8_p719_Accialini http://hdl.handle.net/20.500.12110/paper_1040452X_v84_n8_p719_Accialini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
luteinization progesterone steroidogenesis Tankyrase inhibitor Wnt/β-catenin 7,8 dihydro 2 [4 (trifluoromethyl)phenyl] 5h thiopyrano[4,3 d]pyrimidin 4 ol beta catenin gonadotropin mitogen activated protein kinase progesterone protein kinase B steroidogenic acute regulatory protein tankyrase vasculotropin Wnt protein beta catenin gonadotropin progesterone tankyrase Wnt protein angiogenesis animal experiment animal tissue antral follicle apoptosis Article cell proliferation controlled study corpus luteum enzyme inhibition enzyme phosphorylation female in vivo study luteal cell nonhuman ovary follicle development priority journal progesterone blood level rat steroidogenesis Wnt signaling pathway animal antagonists and inhibitors corpus luteum metabolism physiology signal transduction Animals beta Catenin Corpus Luteum Female Gonadotropins Progesterone Rats Signal Transduction Tankyrases Wnt Proteins |
| spellingShingle |
luteinization progesterone steroidogenesis Tankyrase inhibitor Wnt/β-catenin 7,8 dihydro 2 [4 (trifluoromethyl)phenyl] 5h thiopyrano[4,3 d]pyrimidin 4 ol beta catenin gonadotropin mitogen activated protein kinase progesterone protein kinase B steroidogenic acute regulatory protein tankyrase vasculotropin Wnt protein beta catenin gonadotropin progesterone tankyrase Wnt protein angiogenesis animal experiment animal tissue antral follicle apoptosis Article cell proliferation controlled study corpus luteum enzyme inhibition enzyme phosphorylation female in vivo study luteal cell nonhuman ovary follicle development priority journal progesterone blood level rat steroidogenesis Wnt signaling pathway animal antagonists and inhibitors corpus luteum metabolism physiology signal transduction Animals beta Catenin Corpus Luteum Female Gonadotropins Progesterone Rats Signal Transduction Tankyrases Wnt Proteins Tankyrase inhibition regulates corpus luteum development and luteal function in gonadotropin-treated rats |
| topic_facet |
luteinization progesterone steroidogenesis Tankyrase inhibitor Wnt/β-catenin 7,8 dihydro 2 [4 (trifluoromethyl)phenyl] 5h thiopyrano[4,3 d]pyrimidin 4 ol beta catenin gonadotropin mitogen activated protein kinase progesterone protein kinase B steroidogenic acute regulatory protein tankyrase vasculotropin Wnt protein beta catenin gonadotropin progesterone tankyrase Wnt protein angiogenesis animal experiment animal tissue antral follicle apoptosis Article cell proliferation controlled study corpus luteum enzyme inhibition enzyme phosphorylation female in vivo study luteal cell nonhuman ovary follicle development priority journal progesterone blood level rat steroidogenesis Wnt signaling pathway animal antagonists and inhibitors corpus luteum metabolism physiology signal transduction Animals beta Catenin Corpus Luteum Female Gonadotropins Progesterone Rats Signal Transduction Tankyrases Wnt Proteins |
| description |
Tankyrases are physiological regulators of Axin, a protein involved in several cellular processes, including Wnt signaling. Here, we investigated the effect of a specific Tankyrase inhibitor (XAV939) in follicular-luteal dynamics, and its possible relationship with ovarian vascular development. Studies were designed to analyze the effect of intrabursa administration of XAV939 in gonadotropin-treated prepubertal rats. In particular, we examined follicle and corpus luteum development, steroidogenesis, angiogenic markers, and apoptotic parameters. We found that in vivo inhibition of Wnt signaling impaired corpus luteum development, with a decrease in the number of corpora lutea balanced by a high number of cysts; decreased circulating progesterone levels, likely due to a decrease in Steroidogenic acute regulatory protein content in the corpus luteum; and increased pro-apoptotic parameters. In addition, Extracellular signal-regulated kinase phosphorylation, Vascular endothelium growth factor 120 content, and endothelial cell area were diminished in corpora lutea of inhibitor-treated ovaries. Thus, Wnt/β-catenin signaling appears to participate in the regulation of corpus luteum development and luteal cell function. © 2017 Wiley Periodicals, Inc. |
| title |
Tankyrase inhibition regulates corpus luteum development and luteal function in gonadotropin-treated rats |
| title_short |
Tankyrase inhibition regulates corpus luteum development and luteal function in gonadotropin-treated rats |
| title_full |
Tankyrase inhibition regulates corpus luteum development and luteal function in gonadotropin-treated rats |
| title_fullStr |
Tankyrase inhibition regulates corpus luteum development and luteal function in gonadotropin-treated rats |
| title_full_unstemmed |
Tankyrase inhibition regulates corpus luteum development and luteal function in gonadotropin-treated rats |
| title_sort |
tankyrase inhibition regulates corpus luteum development and luteal function in gonadotropin-treated rats |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v84_n8_p719_Accialini http://hdl.handle.net/20.500.12110/paper_1040452X_v84_n8_p719_Accialini |
| _version_ |
1768545796841537536 |