Local effects of the sphingosine 1-phosphate on prostaglandin F2alpha-induced luteolysis in the pregnant rat

Since the regression of the corpus luteum (CL) occurs via a tightly controlled apoptotic process, studies were designed to determine if local administration of the antiapoptotic agent sphingosine 1-phosphate (S1P) effectively blocks the luteolytic action of prostaglandin F-2alpha (PGF-2α). On day 19...

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Autores principales:	Peluffo, Marina Cinthia, Tesone, Marta
Publicado:	2009
Materias:	caspase 2 caspase 8 caspase 9 progesterone prostaglandin F2 alpha protein kinase B sphingosine 1 phosphate tumor necrosis factor alpha animal cell animal experiment animal tissue article cell death controlled study corpus luteum drug effect electron microscopy enzyme activity enzyme linked immunosorbent assay female luteolysis nonhuman pregnancy priority journal protein expression protein phosphorylation rat Rattus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v76_n12_p1153_Hernandez http://hdl.handle.net/20.500.12110/paper_1040452X_v76_n12_p1153_Hernandez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_1040452X_v76_n12_p1153_Hernandez2023-06-08T16:00:46Z Local effects of the sphingosine 1-phosphate on prostaglandin F2alpha-induced luteolysis in the pregnant rat Peluffo, Marina Cinthia Tesone, Marta caspase 2 caspase 8 caspase 9 progesterone prostaglandin F2 alpha protein kinase B sphingosine 1 phosphate tumor necrosis factor alpha animal cell animal experiment animal tissue article cell death controlled study corpus luteum drug effect electron microscopy enzyme activity enzyme linked immunosorbent assay female luteolysis nonhuman pregnancy priority journal protein expression protein phosphorylation rat Rattus Since the regression of the corpus luteum (CL) occurs via a tightly controlled apoptotic process, studies were designed to determine if local administration of the antiapoptotic agent sphingosine 1-phosphate (S1P) effectively blocks the luteolytic action of prostaglandin F-2alpha (PGF-2α). On day 19 of pregnancy, 2 hr before systemic PGF-2α administration, rats were injected intrabursa with either S1P or vehicle (control). The activity of four caspases, which contribute to the initial (caspase-2, -8, and -9) and final (caspase-3) events in apoptosis was measured in pooled CL from four individual ovaries at 0 and 4 hr after PGF-2α injection. The expression of the phosphorylated form of AKT (pAKT) and tumor necrosis factor-alpha (TNF-α) was analyzed by ELISA. In addition, cell death was evaluated by electronic microscopy (EM) in CL 4 and 36 hr after PGF-2α injection. The activity of caspase-2, -3, and -8 was significantly greater by 4 hr after PGF-2α, but not caspase-9 activity. In contrast, expression of pAKT and TNF-α decreased significantly. Administration of S1P suppressed (P < 0.05) these effects, decreasing caspase activities and increasing pAKT and TNF-α expression. The administration of S1P also significantly decreased the percentage of luteal apoptotic cells induced by PGF-2α. PGF-2α treatment increased the prevalence of luteal cells with advanced signs of apoptosis (i.e., multiple nuclear fragments, chromatin condensation, or apoptotic bodies). S1P treatment suppressed these changes and increased the blood vessel density. These results suggest that S1P blocks the luteolytic effect of the PGF-2α by decreasing caspase-2, -3, and -8 activities and increasing AKT phosphorylation and TNF-α expression. © 2009 Wiley-Liss, Inc. Fil:Peluffo, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v76_n12_p1153_Hernandez http://hdl.handle.net/20.500.12110/paper_1040452X_v76_n12_p1153_Hernandez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	caspase 2 caspase 8 caspase 9 progesterone prostaglandin F2 alpha protein kinase B sphingosine 1 phosphate tumor necrosis factor alpha animal cell animal experiment animal tissue article cell death controlled study corpus luteum drug effect electron microscopy enzyme activity enzyme linked immunosorbent assay female luteolysis nonhuman pregnancy priority journal protein expression protein phosphorylation rat Rattus
spellingShingle	caspase 2 caspase 8 caspase 9 progesterone prostaglandin F2 alpha protein kinase B sphingosine 1 phosphate tumor necrosis factor alpha animal cell animal experiment animal tissue article cell death controlled study corpus luteum drug effect electron microscopy enzyme activity enzyme linked immunosorbent assay female luteolysis nonhuman pregnancy priority journal protein expression protein phosphorylation rat Rattus Peluffo, Marina Cinthia Tesone, Marta Local effects of the sphingosine 1-phosphate on prostaglandin F2alpha-induced luteolysis in the pregnant rat
topic_facet	caspase 2 caspase 8 caspase 9 progesterone prostaglandin F2 alpha protein kinase B sphingosine 1 phosphate tumor necrosis factor alpha animal cell animal experiment animal tissue article cell death controlled study corpus luteum drug effect electron microscopy enzyme activity enzyme linked immunosorbent assay female luteolysis nonhuman pregnancy priority journal protein expression protein phosphorylation rat Rattus
description	Since the regression of the corpus luteum (CL) occurs via a tightly controlled apoptotic process, studies were designed to determine if local administration of the antiapoptotic agent sphingosine 1-phosphate (S1P) effectively blocks the luteolytic action of prostaglandin F-2alpha (PGF-2α). On day 19 of pregnancy, 2 hr before systemic PGF-2α administration, rats were injected intrabursa with either S1P or vehicle (control). The activity of four caspases, which contribute to the initial (caspase-2, -8, and -9) and final (caspase-3) events in apoptosis was measured in pooled CL from four individual ovaries at 0 and 4 hr after PGF-2α injection. The expression of the phosphorylated form of AKT (pAKT) and tumor necrosis factor-alpha (TNF-α) was analyzed by ELISA. In addition, cell death was evaluated by electronic microscopy (EM) in CL 4 and 36 hr after PGF-2α injection. The activity of caspase-2, -3, and -8 was significantly greater by 4 hr after PGF-2α, but not caspase-9 activity. In contrast, expression of pAKT and TNF-α decreased significantly. Administration of S1P suppressed (P < 0.05) these effects, decreasing caspase activities and increasing pAKT and TNF-α expression. The administration of S1P also significantly decreased the percentage of luteal apoptotic cells induced by PGF-2α. PGF-2α treatment increased the prevalence of luteal cells with advanced signs of apoptosis (i.e., multiple nuclear fragments, chromatin condensation, or apoptotic bodies). S1P treatment suppressed these changes and increased the blood vessel density. These results suggest that S1P blocks the luteolytic effect of the PGF-2α by decreasing caspase-2, -3, and -8 activities and increasing AKT phosphorylation and TNF-α expression. © 2009 Wiley-Liss, Inc.
author	Peluffo, Marina Cinthia Tesone, Marta
author_facet	Peluffo, Marina Cinthia Tesone, Marta
author_sort	Peluffo, Marina Cinthia
title	Local effects of the sphingosine 1-phosphate on prostaglandin F2alpha-induced luteolysis in the pregnant rat
title_short	Local effects of the sphingosine 1-phosphate on prostaglandin F2alpha-induced luteolysis in the pregnant rat
title_full	Local effects of the sphingosine 1-phosphate on prostaglandin F2alpha-induced luteolysis in the pregnant rat
title_fullStr	Local effects of the sphingosine 1-phosphate on prostaglandin F2alpha-induced luteolysis in the pregnant rat
title_full_unstemmed	Local effects of the sphingosine 1-phosphate on prostaglandin F2alpha-induced luteolysis in the pregnant rat
title_sort	local effects of the sphingosine 1-phosphate on prostaglandin f2alpha-induced luteolysis in the pregnant rat
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v76_n12_p1153_Hernandez http://hdl.handle.net/20.500.12110/paper_1040452X_v76_n12_p1153_Hernandez
work_keys_str_mv	AT peluffomarinacinthia localeffectsofthesphingosine1phosphateonprostaglandinf2alphainducedluteolysisinthepregnantrat AT tesonemarta localeffectsofthesphingosine1phosphateonprostaglandinf2alphainducedluteolysisinthepregnantrat
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Local effects of the sphingosine 1-phosphate on prostaglandin F2alpha-induced luteolysis in the pregnant rat

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