Regulation of ovarian angiogenesis and apoptosis by GnRH-I analogs

An adequate vascular supply is important to provide endocrine and paracrine signals during follicular development. We evaluated the direct in vivo effects of both the GnRH-agonist Leuprolide acetate (LA) and the GnRH-antagonist Antide (Ant) on the expression of VEGF-A and ANPT-1 and their receptors...

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Autores principales:	Parborell, M.Fernanda A., Irusta, Griselda, Tesone, Marta
Publicado:	2008
Materias:	Angiogenesis Apoptosis GnRH-I analogs Ovary angiopoietin receptor caspase 3 iturelix leuprorelin vasculotropin A vasculotropin receptor 2 angiogenesis apoptosis article controlled study enzyme activity female immunochemistry in vivo study nonhuman ovary ovary follicle priority journal protein expression rat Angiopoietin-1 Animals Caspase 3 Enzyme Activation Female Gonadotropin-Releasing Hormone Injections, Subcutaneous Leuprolide Neovascularization, Physiologic Oligopeptides Ovarian Follicle Rats Rats, Sprague-Dawley Receptor, TIE-2 Structure-Activity Relationship Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 Rattus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v75_n4_p623_Parborell http://hdl.handle.net/20.500.12110/paper_1040452X_v75_n4_p623_Parborell
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_1040452X_v75_n4_p623_Parborell
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spelling	paper:paper_1040452X_v75_n4_p623_Parborell2023-06-08T16:00:45Z Regulation of ovarian angiogenesis and apoptosis by GnRH-I analogs Parborell, M.Fernanda A. Irusta, Griselda Tesone, Marta Angiogenesis Apoptosis GnRH-I analogs Ovary angiopoietin receptor caspase 3 iturelix leuprorelin vasculotropin A vasculotropin receptor 2 angiogenesis apoptosis article controlled study enzyme activity female immunochemistry in vivo study nonhuman ovary ovary follicle priority journal protein expression rat Angiopoietin-1 Animals Apoptosis Caspase 3 Enzyme Activation Female Gonadotropin-Releasing Hormone Injections, Subcutaneous Leuprolide Neovascularization, Physiologic Oligopeptides Ovarian Follicle Ovary Rats Rats, Sprague-Dawley Receptor, TIE-2 Structure-Activity Relationship Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 Rattus An adequate vascular supply is important to provide endocrine and paracrine signals during follicular development. We evaluated the direct in vivo effects of both the GnRH-agonist Leuprolide acetate (LA) and the GnRH-antagonist Antide (Ant) on the expression of VEGF-A and ANPT-1 and their receptors in ovarian follicles from prepubertal eCG-treated rats. We also examined whether the changes observed in apoptosis by GnRH-I analogs have an effect on the caspase cascade. LA significantly decreased the levels of VEGF-A, its receptor Flk-1, and ANPT-1 when compared to controls, while the co-injection of Ant interfered with this effect. No changes were observed in the levels of Tie-2 after treatment with these analogs. When we measured the follicular content of caspase-3 protein, we observed that LA significantly increased the level of the active form. The co-injection of Ant interfered with this effect and Ant alone significantly decreased caspase-3 cleavage. IHC analyses corroborated these data. Notably, while LA increased caspase-3 activity levels, Ant decreased them when compared to controls. In follicles obtained from LA-treated rats, cleavage of PARP (a substrate of caspase-3) from the intact 113-kDa protein showed a significant enhancement in an 85-kDa fragment. The co-injection of Ant interfered with this effect. Ant alone significantly decreased PARP cleavage as compared to controls. We conclude that the decrease in VEGF-A, its receptor Flk-1/KDR, and ANPT-1 produced by the administration of GnRH-1 agonist is one of the mechanisms involved in ovarian cell apoptosis. This suggests an intraovarian role of an endogenous GnRH-like peptide in gonadotropin-induced follicular development. © 2007 Wiley-Liss, Inc. Fil:Parborell, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Irusta, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v75_n4_p623_Parborell http://hdl.handle.net/20.500.12110/paper_1040452X_v75_n4_p623_Parborell
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Angiogenesis Apoptosis GnRH-I analogs Ovary angiopoietin receptor caspase 3 iturelix leuprorelin vasculotropin A vasculotropin receptor 2 angiogenesis apoptosis article controlled study enzyme activity female immunochemistry in vivo study nonhuman ovary ovary follicle priority journal protein expression rat Angiopoietin-1 Animals Apoptosis Caspase 3 Enzyme Activation Female Gonadotropin-Releasing Hormone Injections, Subcutaneous Leuprolide Neovascularization, Physiologic Oligopeptides Ovarian Follicle Ovary Rats Rats, Sprague-Dawley Receptor, TIE-2 Structure-Activity Relationship Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 Rattus
spellingShingle	Angiogenesis Apoptosis GnRH-I analogs Ovary angiopoietin receptor caspase 3 iturelix leuprorelin vasculotropin A vasculotropin receptor 2 angiogenesis apoptosis article controlled study enzyme activity female immunochemistry in vivo study nonhuman ovary ovary follicle priority journal protein expression rat Angiopoietin-1 Animals Apoptosis Caspase 3 Enzyme Activation Female Gonadotropin-Releasing Hormone Injections, Subcutaneous Leuprolide Neovascularization, Physiologic Oligopeptides Ovarian Follicle Ovary Rats Rats, Sprague-Dawley Receptor, TIE-2 Structure-Activity Relationship Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 Rattus Parborell, M.Fernanda A. Irusta, Griselda Tesone, Marta Regulation of ovarian angiogenesis and apoptosis by GnRH-I analogs
topic_facet	Angiogenesis Apoptosis GnRH-I analogs Ovary angiopoietin receptor caspase 3 iturelix leuprorelin vasculotropin A vasculotropin receptor 2 angiogenesis apoptosis article controlled study enzyme activity female immunochemistry in vivo study nonhuman ovary ovary follicle priority journal protein expression rat Angiopoietin-1 Animals Apoptosis Caspase 3 Enzyme Activation Female Gonadotropin-Releasing Hormone Injections, Subcutaneous Leuprolide Neovascularization, Physiologic Oligopeptides Ovarian Follicle Ovary Rats Rats, Sprague-Dawley Receptor, TIE-2 Structure-Activity Relationship Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor-2 Rattus
description	An adequate vascular supply is important to provide endocrine and paracrine signals during follicular development. We evaluated the direct in vivo effects of both the GnRH-agonist Leuprolide acetate (LA) and the GnRH-antagonist Antide (Ant) on the expression of VEGF-A and ANPT-1 and their receptors in ovarian follicles from prepubertal eCG-treated rats. We also examined whether the changes observed in apoptosis by GnRH-I analogs have an effect on the caspase cascade. LA significantly decreased the levels of VEGF-A, its receptor Flk-1, and ANPT-1 when compared to controls, while the co-injection of Ant interfered with this effect. No changes were observed in the levels of Tie-2 after treatment with these analogs. When we measured the follicular content of caspase-3 protein, we observed that LA significantly increased the level of the active form. The co-injection of Ant interfered with this effect and Ant alone significantly decreased caspase-3 cleavage. IHC analyses corroborated these data. Notably, while LA increased caspase-3 activity levels, Ant decreased them when compared to controls. In follicles obtained from LA-treated rats, cleavage of PARP (a substrate of caspase-3) from the intact 113-kDa protein showed a significant enhancement in an 85-kDa fragment. The co-injection of Ant interfered with this effect. Ant alone significantly decreased PARP cleavage as compared to controls. We conclude that the decrease in VEGF-A, its receptor Flk-1/KDR, and ANPT-1 produced by the administration of GnRH-1 agonist is one of the mechanisms involved in ovarian cell apoptosis. This suggests an intraovarian role of an endogenous GnRH-like peptide in gonadotropin-induced follicular development. © 2007 Wiley-Liss, Inc.
author	Parborell, M.Fernanda A. Irusta, Griselda Tesone, Marta
author_facet	Parborell, M.Fernanda A. Irusta, Griselda Tesone, Marta
author_sort	Parborell, M.Fernanda A.
title	Regulation of ovarian angiogenesis and apoptosis by GnRH-I analogs
title_short	Regulation of ovarian angiogenesis and apoptosis by GnRH-I analogs
title_full	Regulation of ovarian angiogenesis and apoptosis by GnRH-I analogs
title_fullStr	Regulation of ovarian angiogenesis and apoptosis by GnRH-I analogs
title_full_unstemmed	Regulation of ovarian angiogenesis and apoptosis by GnRH-I analogs
title_sort	regulation of ovarian angiogenesis and apoptosis by gnrh-i analogs
publishDate	2008
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v75_n4_p623_Parborell http://hdl.handle.net/20.500.12110/paper_1040452X_v75_n4_p623_Parborell
work_keys_str_mv	AT parborellmfernandaa regulationofovarianangiogenesisandapoptosisbygnrhianalogs AT irustagriselda regulationofovarianangiogenesisandapoptosisbygnrhianalogs AT tesonemarta regulationofovarianangiogenesisandapoptosisbygnrhianalogs
_version_	1768541855878742016

Regulation of ovarian angiogenesis and apoptosis by GnRH-I analogs

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