Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis
Apoptosis is the biological process by which follicular cells are eliminated in atretic follicles. The aim of the present study was to examine the in vitro effect of a GnRH-a (leuprolide acetate, LA) and its interactions with FSH, dibutyryl cAMP, and growth factors (IGF-I, EGF, and FGF) on follicula...
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2001
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v60_n2_p241_Parborell http://hdl.handle.net/20.500.12110/paper_1040452X_v60_n2_p241_Parborell |
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paper:paper_1040452X_v60_n2_p241_Parborell2023-06-08T16:00:44Z Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis Parborell, M.Fernanda A. Tesone, Marta Apoptosis Early antral follicles GnRH Growth factors Ovary diethylstilbestrol DNA fragment follitropin gonadorelin agonist growth factor leuprorelin animal cell apoptosis article cell culture controlled study DNA cleavage female in vitro study nonhuman ovary development ovary follicle prepuberty priority journal rat Animals Apoptosis Bucladesine Cells, Cultured Culture Media, Serum-Free Diethylstilbestrol DNA Fragmentation Epidermal Growth Factor Estrogens, Non-Steroidal Female Fertility Agents, Female Fibroblast Growth Factors Follicle Stimulating Hormone Follicular Atresia Gonadotropin-Releasing Hormone Insulin-Like Growth Factor I Leuprolide Ovarian Follicle Rats Rats, Sprague-Dawley Sexual Maturation Apoptosis is the biological process by which follicular cells are eliminated in atretic follicles. The aim of the present study was to examine the in vitro effect of a GnRH-a (leuprolide acetate, LA) and its interactions with FSH, dibutyryl cAMP, and growth factors (IGF-I, EGF, and FGF) on follicular apoptosis in early antral ovarian follicles obtained from prepubertal DES-treated rats. Follicles cultured 24 hr in the absence of hormones showed spontaneous onset of apoptotic DNA fragmentation. The presence of FSH suppressed the spontaneous onset of apoptotic DNA fragmentation (75-85%). Quantitative estimation of DNA cleavage from ovarian follicles revealed no significant changes in DNA fragmentation after in vitro LA treatment (1-100 ng/ml). However, coincubation with LA interfered partially with the effects of FSH on apoptosis suppression. This apoptosis suppression was also obtained by treatment with dibutyryl cAMP (80%), and was partially prevented by the presence of LA in the cultures. Follicles were cultured 24 hr with FGF, EGF, or IGF-I, and these factors suppressed DNA fragmentation (70, 60, and 70% respectively), while the presence of LA (100 ng/ml) in the culture medium prevented this effect. In conclusion, we show that the rescue from apoptotic DNA fragmentation produced in early antral follicles by FSH, cAMP, and growth factors, is prevented by coincubation with LA. This GnRH analog would thus interfere in the pathway of FSH, cAMP and/or growth factors by an as yet unknown mechanism. © 2001 Wiley-Liss, Inc. Fil:Parborell, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v60_n2_p241_Parborell http://hdl.handle.net/20.500.12110/paper_1040452X_v60_n2_p241_Parborell |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Apoptosis Early antral follicles GnRH Growth factors Ovary diethylstilbestrol DNA fragment follitropin gonadorelin agonist growth factor leuprorelin animal cell apoptosis article cell culture controlled study DNA cleavage female in vitro study nonhuman ovary development ovary follicle prepuberty priority journal rat Animals Apoptosis Bucladesine Cells, Cultured Culture Media, Serum-Free Diethylstilbestrol DNA Fragmentation Epidermal Growth Factor Estrogens, Non-Steroidal Female Fertility Agents, Female Fibroblast Growth Factors Follicle Stimulating Hormone Follicular Atresia Gonadotropin-Releasing Hormone Insulin-Like Growth Factor I Leuprolide Ovarian Follicle Rats Rats, Sprague-Dawley Sexual Maturation |
spellingShingle |
Apoptosis Early antral follicles GnRH Growth factors Ovary diethylstilbestrol DNA fragment follitropin gonadorelin agonist growth factor leuprorelin animal cell apoptosis article cell culture controlled study DNA cleavage female in vitro study nonhuman ovary development ovary follicle prepuberty priority journal rat Animals Apoptosis Bucladesine Cells, Cultured Culture Media, Serum-Free Diethylstilbestrol DNA Fragmentation Epidermal Growth Factor Estrogens, Non-Steroidal Female Fertility Agents, Female Fibroblast Growth Factors Follicle Stimulating Hormone Follicular Atresia Gonadotropin-Releasing Hormone Insulin-Like Growth Factor I Leuprolide Ovarian Follicle Rats Rats, Sprague-Dawley Sexual Maturation Parborell, M.Fernanda A. Tesone, Marta Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis |
topic_facet |
Apoptosis Early antral follicles GnRH Growth factors Ovary diethylstilbestrol DNA fragment follitropin gonadorelin agonist growth factor leuprorelin animal cell apoptosis article cell culture controlled study DNA cleavage female in vitro study nonhuman ovary development ovary follicle prepuberty priority journal rat Animals Apoptosis Bucladesine Cells, Cultured Culture Media, Serum-Free Diethylstilbestrol DNA Fragmentation Epidermal Growth Factor Estrogens, Non-Steroidal Female Fertility Agents, Female Fibroblast Growth Factors Follicle Stimulating Hormone Follicular Atresia Gonadotropin-Releasing Hormone Insulin-Like Growth Factor I Leuprolide Ovarian Follicle Rats Rats, Sprague-Dawley Sexual Maturation |
description |
Apoptosis is the biological process by which follicular cells are eliminated in atretic follicles. The aim of the present study was to examine the in vitro effect of a GnRH-a (leuprolide acetate, LA) and its interactions with FSH, dibutyryl cAMP, and growth factors (IGF-I, EGF, and FGF) on follicular apoptosis in early antral ovarian follicles obtained from prepubertal DES-treated rats. Follicles cultured 24 hr in the absence of hormones showed spontaneous onset of apoptotic DNA fragmentation. The presence of FSH suppressed the spontaneous onset of apoptotic DNA fragmentation (75-85%). Quantitative estimation of DNA cleavage from ovarian follicles revealed no significant changes in DNA fragmentation after in vitro LA treatment (1-100 ng/ml). However, coincubation with LA interfered partially with the effects of FSH on apoptosis suppression. This apoptosis suppression was also obtained by treatment with dibutyryl cAMP (80%), and was partially prevented by the presence of LA in the cultures. Follicles were cultured 24 hr with FGF, EGF, or IGF-I, and these factors suppressed DNA fragmentation (70, 60, and 70% respectively), while the presence of LA (100 ng/ml) in the culture medium prevented this effect. In conclusion, we show that the rescue from apoptotic DNA fragmentation produced in early antral follicles by FSH, cAMP, and growth factors, is prevented by coincubation with LA. This GnRH analog would thus interfere in the pathway of FSH, cAMP and/or growth factors by an as yet unknown mechanism. © 2001 Wiley-Liss, Inc. |
author |
Parborell, M.Fernanda A. Tesone, Marta |
author_facet |
Parborell, M.Fernanda A. Tesone, Marta |
author_sort |
Parborell, M.Fernanda A. |
title |
Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis |
title_short |
Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis |
title_full |
Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis |
title_fullStr |
Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis |
title_full_unstemmed |
Gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with FSH and growth factors on the prevention of apoptosis |
title_sort |
gonadotropin-releasing hormone agonist affects rat ovarian follicle development by interfering with fsh and growth factors on the prevention of apoptosis |
publishDate |
2001 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v60_n2_p241_Parborell http://hdl.handle.net/20.500.12110/paper_1040452X_v60_n2_p241_Parborell |
work_keys_str_mv |
AT parborellmfernandaa gonadotropinreleasinghormoneagonistaffectsratovarianfollicledevelopmentbyinterferingwithfshandgrowthfactorsonthepreventionofapoptosis AT tesonemarta gonadotropinreleasinghormoneagonistaffectsratovarianfollicledevelopmentbyinterferingwithfshandgrowthfactorsonthepreventionofapoptosis |
_version_ |
1768543286801203200 |