Accurate spatial and temporal transgene expression driven by a 3.8- kilobase promoter of the bovine β-casein gene in the lactating mouse mammary gland
The spatial, temporal, and hormonal pattern of expression of the β- casein gene is highly regulated and confined to the epithelial cells of the lactating mammary gland. Previous studies have shown that 1.7 kb of the bovine β-casein promoter were able to drive cell-specific and hormone- dependent exp...
Guardado en:
Autores principales: | , , , , , |
---|---|
Publicado: |
1998
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v49_n3_p236_Cerdan http://hdl.handle.net/20.500.12110/paper_1040452X_v49_n3_p236_Cerdan |
Aporte de: |
id |
paper:paper_1040452X_v49_n3_p236_Cerdan |
---|---|
record_format |
dspace |
spelling |
paper:paper_1040452X_v49_n3_p236_Cerdan2023-06-08T16:00:43Z Accurate spatial and temporal transgene expression driven by a 3.8- kilobase promoter of the bovine β-casein gene in the lactating mouse mammary gland Cerdán, Marcelo Guido Young, Juan I. Falzone, Tomás Luis Otero Corchón, Verónica Torres, Héctor Norberto Rubinstein, Marcelo Casein genes Gene expression Human growth hormone Milk Transgenic mice beta casein growth factor growth hormone animal cell animal experiment article cattle gene expression gene expression regulation lactation mammary gland function mouse nonhuman northern blotting priority journal promoter region radioimmunoassay tissue specificity transgene Animals Caseins Cattle Female Gene Expression Human Growth Hormone Humans Lactation Male Mammary Glands, Animal Mice Mice, Inbred C57BL Mice, Transgenic Milk Promoter Regions (Genetics) Tissue Distribution Transgenes Animalia Bos taurus Bovinae Murinae Mus musculus Rodentia The spatial, temporal, and hormonal pattern of expression of the β- casein gene is highly regulated and confined to the epithelial cells of the lactating mammary gland. Previous studies have shown that 1.7 kb of the bovine β-casein promoter were able to drive cell-specific and hormone- dependent expression to a mouse mammary cell line but failed to induce accurate expression to the mammary gland of transgenic mice. We investigated here the ability of 3.8 kb of the bovine β-casein gene promoter to drive the expression of the human growth hormone (hGH) gene in transgenic mice. A Northern blot analysis using total RNA obtained from different tissues of lactating and nonlactating females revealed the presence of hGH mRNA only in the mammary gland of lactating females. hGH mRNA was not detectable in the mammary gland of virgin females or males. A developmental analysis showed that hGH mRNA only peaked on parturition, resembling more closely the bovine β-casein temporal expression pattern rather than the murine. In situ hibridization studies performed on mammary gland sections showed that the cellular pattern of hGH expression was homogeneous in all lobules from heterozygous and homozygous transgenic mice. Silver grain counts on the tissue sections highly correlated with the hGH contents in the milk determined by radioimmunoassay (r = 0.996). Thus 3.8 kb of the bovine β- casein promoter direct a high-level expression of a reporter gene to the lactating mammary gland of transgenic mice in a tissue-specific and developmentally regulated manner. Fil:Cerdán, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Young, J.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Falzone, T.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Otero, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rubinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v49_n3_p236_Cerdan http://hdl.handle.net/20.500.12110/paper_1040452X_v49_n3_p236_Cerdan |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Casein genes Gene expression Human growth hormone Milk Transgenic mice beta casein growth factor growth hormone animal cell animal experiment article cattle gene expression gene expression regulation lactation mammary gland function mouse nonhuman northern blotting priority journal promoter region radioimmunoassay tissue specificity transgene Animals Caseins Cattle Female Gene Expression Human Growth Hormone Humans Lactation Male Mammary Glands, Animal Mice Mice, Inbred C57BL Mice, Transgenic Milk Promoter Regions (Genetics) Tissue Distribution Transgenes Animalia Bos taurus Bovinae Murinae Mus musculus Rodentia |
spellingShingle |
Casein genes Gene expression Human growth hormone Milk Transgenic mice beta casein growth factor growth hormone animal cell animal experiment article cattle gene expression gene expression regulation lactation mammary gland function mouse nonhuman northern blotting priority journal promoter region radioimmunoassay tissue specificity transgene Animals Caseins Cattle Female Gene Expression Human Growth Hormone Humans Lactation Male Mammary Glands, Animal Mice Mice, Inbred C57BL Mice, Transgenic Milk Promoter Regions (Genetics) Tissue Distribution Transgenes Animalia Bos taurus Bovinae Murinae Mus musculus Rodentia Cerdán, Marcelo Guido Young, Juan I. Falzone, Tomás Luis Otero Corchón, Verónica Torres, Héctor Norberto Rubinstein, Marcelo Accurate spatial and temporal transgene expression driven by a 3.8- kilobase promoter of the bovine β-casein gene in the lactating mouse mammary gland |
topic_facet |
Casein genes Gene expression Human growth hormone Milk Transgenic mice beta casein growth factor growth hormone animal cell animal experiment article cattle gene expression gene expression regulation lactation mammary gland function mouse nonhuman northern blotting priority journal promoter region radioimmunoassay tissue specificity transgene Animals Caseins Cattle Female Gene Expression Human Growth Hormone Humans Lactation Male Mammary Glands, Animal Mice Mice, Inbred C57BL Mice, Transgenic Milk Promoter Regions (Genetics) Tissue Distribution Transgenes Animalia Bos taurus Bovinae Murinae Mus musculus Rodentia |
description |
The spatial, temporal, and hormonal pattern of expression of the β- casein gene is highly regulated and confined to the epithelial cells of the lactating mammary gland. Previous studies have shown that 1.7 kb of the bovine β-casein promoter were able to drive cell-specific and hormone- dependent expression to a mouse mammary cell line but failed to induce accurate expression to the mammary gland of transgenic mice. We investigated here the ability of 3.8 kb of the bovine β-casein gene promoter to drive the expression of the human growth hormone (hGH) gene in transgenic mice. A Northern blot analysis using total RNA obtained from different tissues of lactating and nonlactating females revealed the presence of hGH mRNA only in the mammary gland of lactating females. hGH mRNA was not detectable in the mammary gland of virgin females or males. A developmental analysis showed that hGH mRNA only peaked on parturition, resembling more closely the bovine β-casein temporal expression pattern rather than the murine. In situ hibridization studies performed on mammary gland sections showed that the cellular pattern of hGH expression was homogeneous in all lobules from heterozygous and homozygous transgenic mice. Silver grain counts on the tissue sections highly correlated with the hGH contents in the milk determined by radioimmunoassay (r = 0.996). Thus 3.8 kb of the bovine β- casein promoter direct a high-level expression of a reporter gene to the lactating mammary gland of transgenic mice in a tissue-specific and developmentally regulated manner. |
author |
Cerdán, Marcelo Guido Young, Juan I. Falzone, Tomás Luis Otero Corchón, Verónica Torres, Héctor Norberto Rubinstein, Marcelo |
author_facet |
Cerdán, Marcelo Guido Young, Juan I. Falzone, Tomás Luis Otero Corchón, Verónica Torres, Héctor Norberto Rubinstein, Marcelo |
author_sort |
Cerdán, Marcelo Guido |
title |
Accurate spatial and temporal transgene expression driven by a 3.8- kilobase promoter of the bovine β-casein gene in the lactating mouse mammary gland |
title_short |
Accurate spatial and temporal transgene expression driven by a 3.8- kilobase promoter of the bovine β-casein gene in the lactating mouse mammary gland |
title_full |
Accurate spatial and temporal transgene expression driven by a 3.8- kilobase promoter of the bovine β-casein gene in the lactating mouse mammary gland |
title_fullStr |
Accurate spatial and temporal transgene expression driven by a 3.8- kilobase promoter of the bovine β-casein gene in the lactating mouse mammary gland |
title_full_unstemmed |
Accurate spatial and temporal transgene expression driven by a 3.8- kilobase promoter of the bovine β-casein gene in the lactating mouse mammary gland |
title_sort |
accurate spatial and temporal transgene expression driven by a 3.8- kilobase promoter of the bovine β-casein gene in the lactating mouse mammary gland |
publishDate |
1998 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1040452X_v49_n3_p236_Cerdan http://hdl.handle.net/20.500.12110/paper_1040452X_v49_n3_p236_Cerdan |
work_keys_str_mv |
AT cerdanmarceloguido accuratespatialandtemporaltransgeneexpressiondrivenbya38kilobasepromoterofthebovinebcaseingeneinthelactatingmousemammarygland AT youngjuani accuratespatialandtemporaltransgeneexpressiondrivenbya38kilobasepromoterofthebovinebcaseingeneinthelactatingmousemammarygland AT falzonetomasluis accuratespatialandtemporaltransgeneexpressiondrivenbya38kilobasepromoterofthebovinebcaseingeneinthelactatingmousemammarygland AT oterocorchonveronica accuratespatialandtemporaltransgeneexpressiondrivenbya38kilobasepromoterofthebovinebcaseingeneinthelactatingmousemammarygland AT torreshectornorberto accuratespatialandtemporaltransgeneexpressiondrivenbya38kilobasepromoterofthebovinebcaseingeneinthelactatingmousemammarygland AT rubinsteinmarcelo accuratespatialandtemporaltransgeneexpressiondrivenbya38kilobasepromoterofthebovinebcaseingeneinthelactatingmousemammarygland |
_version_ |
1768544742061113344 |