Combined Effects of Simultaneous Exposure to Caffeine and Cocaine in the Mouse Striatum
Caffeine is the world’s most popular psychoactive drug and is also an active adulterant found in many drugs of abuse, including seized cocaine samples. Despite several studies which examine the effects of caffeine or cocaine administered as single agents, little data are available for these agents w...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10298428_v29_n4_p525_Muniz http://hdl.handle.net/20.500.12110/paper_10298428_v29_n4_p525_Muniz |
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paper:paper_10298428_v29_n4_p525_Muniz2023-06-08T16:00:21Z Combined Effects of Simultaneous Exposure to Caffeine and Cocaine in the Mouse Striatum Astroglia Caffeine Cocaine Dopamine Locomotor sensitization Striatum adenosine A2a receptor alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid caffeine cocaine dopamine 1 receptor dopamine 2 receptor glial fibrillary acidic protein glutamate receptor messenger RNA n methyl dextro aspartic acid caffeine central stimulant agent cocaine dopamine uptake inhibitor drug combination glial fibrillary acidic protein membrane protein messenger RNA nerve protein animal experiment animal tissue Article astrocytosis brain function controlled study corpus striatum down regulation gene expression genetic analysis immunohistochemistry immunoreactivity locomotion maladjustment male mouse nerve cell plasticity nonhuman priority journal sensitization upregulation analysis of variance animal C57BL mouse corpus striatum drug combination drug effects gene expression regulation genetics metabolism time factor Analysis of Variance Animals Caffeine Central Nervous System Stimulants Cocaine Corpus Striatum Dopamine Uptake Inhibitors Drug Combinations Gene Expression Regulation Glial Fibrillary Acidic Protein Locomotion Male Membrane Proteins Mice Mice, Inbred C57BL Nerve Tissue Proteins RNA, Messenger Time Factors Caffeine is the world’s most popular psychoactive drug and is also an active adulterant found in many drugs of abuse, including seized cocaine samples. Despite several studies which examine the effects of caffeine or cocaine administered as single agents, little data are available for these agents when given in combination. The purpose of the present study was to determine if combined intake of both psychostimulants can lead to maladaptive changes in striatal function. Mice were injected with a binge regimen (intermittent treatment for 13 days) of caffeine (3 × 5 mg/kg), cocaine (3 × 10 mg/kg), or combined administration. We found that chronic caffeine potentiated locomotion induced by cocaine and that both caffeine-treated groups showed sensitization. Striatal tissue was obtained 24 h and 7 days after last injection (withdrawal) for immunohistochemistry and mRNA expression. Our results show that combined intake of both psychostimulants can increase GFAP immunoreactivity in the striatum at both times post treatment. Gene expression analysis, targeted at dopamine, adenosine, and glutamate receptor subunit genes, revealed significant transcript down-regulation in the dorsal striatum of AMPA, NMDA, D1 and D2 receptor subunit mRNA expression in the group that received combined treatment, but not after individual administration. At withdrawal, we found increased D1 receptor mRNA expression along with increased A1, AMPA, NMDA, and metabotropic subunit expression. A2A mRNA showed decreased expression after both times in all experimental groups. Our study provides evidence that there are striatal alterations mediated by combined caffeine and cocaine administration, and highlights negative outcomes of chronic intake of both psychostimulants. © 2016, Springer Science+Business Media New York. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10298428_v29_n4_p525_Muniz http://hdl.handle.net/20.500.12110/paper_10298428_v29_n4_p525_Muniz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Astroglia Caffeine Cocaine Dopamine Locomotor sensitization Striatum adenosine A2a receptor alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid caffeine cocaine dopamine 1 receptor dopamine 2 receptor glial fibrillary acidic protein glutamate receptor messenger RNA n methyl dextro aspartic acid caffeine central stimulant agent cocaine dopamine uptake inhibitor drug combination glial fibrillary acidic protein membrane protein messenger RNA nerve protein animal experiment animal tissue Article astrocytosis brain function controlled study corpus striatum down regulation gene expression genetic analysis immunohistochemistry immunoreactivity locomotion maladjustment male mouse nerve cell plasticity nonhuman priority journal sensitization upregulation analysis of variance animal C57BL mouse corpus striatum drug combination drug effects gene expression regulation genetics metabolism time factor Analysis of Variance Animals Caffeine Central Nervous System Stimulants Cocaine Corpus Striatum Dopamine Uptake Inhibitors Drug Combinations Gene Expression Regulation Glial Fibrillary Acidic Protein Locomotion Male Membrane Proteins Mice Mice, Inbred C57BL Nerve Tissue Proteins RNA, Messenger Time Factors |
| spellingShingle |
Astroglia Caffeine Cocaine Dopamine Locomotor sensitization Striatum adenosine A2a receptor alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid caffeine cocaine dopamine 1 receptor dopamine 2 receptor glial fibrillary acidic protein glutamate receptor messenger RNA n methyl dextro aspartic acid caffeine central stimulant agent cocaine dopamine uptake inhibitor drug combination glial fibrillary acidic protein membrane protein messenger RNA nerve protein animal experiment animal tissue Article astrocytosis brain function controlled study corpus striatum down regulation gene expression genetic analysis immunohistochemistry immunoreactivity locomotion maladjustment male mouse nerve cell plasticity nonhuman priority journal sensitization upregulation analysis of variance animal C57BL mouse corpus striatum drug combination drug effects gene expression regulation genetics metabolism time factor Analysis of Variance Animals Caffeine Central Nervous System Stimulants Cocaine Corpus Striatum Dopamine Uptake Inhibitors Drug Combinations Gene Expression Regulation Glial Fibrillary Acidic Protein Locomotion Male Membrane Proteins Mice Mice, Inbred C57BL Nerve Tissue Proteins RNA, Messenger Time Factors Combined Effects of Simultaneous Exposure to Caffeine and Cocaine in the Mouse Striatum |
| topic_facet |
Astroglia Caffeine Cocaine Dopamine Locomotor sensitization Striatum adenosine A2a receptor alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid caffeine cocaine dopamine 1 receptor dopamine 2 receptor glial fibrillary acidic protein glutamate receptor messenger RNA n methyl dextro aspartic acid caffeine central stimulant agent cocaine dopamine uptake inhibitor drug combination glial fibrillary acidic protein membrane protein messenger RNA nerve protein animal experiment animal tissue Article astrocytosis brain function controlled study corpus striatum down regulation gene expression genetic analysis immunohistochemistry immunoreactivity locomotion maladjustment male mouse nerve cell plasticity nonhuman priority journal sensitization upregulation analysis of variance animal C57BL mouse corpus striatum drug combination drug effects gene expression regulation genetics metabolism time factor Analysis of Variance Animals Caffeine Central Nervous System Stimulants Cocaine Corpus Striatum Dopamine Uptake Inhibitors Drug Combinations Gene Expression Regulation Glial Fibrillary Acidic Protein Locomotion Male Membrane Proteins Mice Mice, Inbred C57BL Nerve Tissue Proteins RNA, Messenger Time Factors |
| description |
Caffeine is the world’s most popular psychoactive drug and is also an active adulterant found in many drugs of abuse, including seized cocaine samples. Despite several studies which examine the effects of caffeine or cocaine administered as single agents, little data are available for these agents when given in combination. The purpose of the present study was to determine if combined intake of both psychostimulants can lead to maladaptive changes in striatal function. Mice were injected with a binge regimen (intermittent treatment for 13 days) of caffeine (3 × 5 mg/kg), cocaine (3 × 10 mg/kg), or combined administration. We found that chronic caffeine potentiated locomotion induced by cocaine and that both caffeine-treated groups showed sensitization. Striatal tissue was obtained 24 h and 7 days after last injection (withdrawal) for immunohistochemistry and mRNA expression. Our results show that combined intake of both psychostimulants can increase GFAP immunoreactivity in the striatum at both times post treatment. Gene expression analysis, targeted at dopamine, adenosine, and glutamate receptor subunit genes, revealed significant transcript down-regulation in the dorsal striatum of AMPA, NMDA, D1 and D2 receptor subunit mRNA expression in the group that received combined treatment, but not after individual administration. At withdrawal, we found increased D1 receptor mRNA expression along with increased A1, AMPA, NMDA, and metabotropic subunit expression. A2A mRNA showed decreased expression after both times in all experimental groups. Our study provides evidence that there are striatal alterations mediated by combined caffeine and cocaine administration, and highlights negative outcomes of chronic intake of both psychostimulants. © 2016, Springer Science+Business Media New York. |
| title |
Combined Effects of Simultaneous Exposure to Caffeine and Cocaine in the Mouse Striatum |
| title_short |
Combined Effects of Simultaneous Exposure to Caffeine and Cocaine in the Mouse Striatum |
| title_full |
Combined Effects of Simultaneous Exposure to Caffeine and Cocaine in the Mouse Striatum |
| title_fullStr |
Combined Effects of Simultaneous Exposure to Caffeine and Cocaine in the Mouse Striatum |
| title_full_unstemmed |
Combined Effects of Simultaneous Exposure to Caffeine and Cocaine in the Mouse Striatum |
| title_sort |
combined effects of simultaneous exposure to caffeine and cocaine in the mouse striatum |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10298428_v29_n4_p525_Muniz http://hdl.handle.net/20.500.12110/paper_10298428_v29_n4_p525_Muniz |
| _version_ |
1768542326240575488 |