Aeromonas hydrophila strains as biocatalysts for transglycosylation
Microbial transglycosylation is useful as a green alternative in the preparation of purine nucleosides and analogues, especially for those that display pharmacological activities. In a search for new transglycosylation biocatalysts, two Aeromonas hydrophila strains were selected. The substrate speci...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10242422_v28_n5-6_p395_Nbile http://hdl.handle.net/20.500.12110/paper_10242422_v28_n5-6_p395_Nbile |
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paper:paper_10242422_v28_n5-6_p395_Nbile2023-06-08T16:00:12Z Aeromonas hydrophila strains as biocatalysts for transglycosylation Aeromonas hydrophila Didanosine Microbial transglycosylation Ribavirin Whole cell biocatalysts Aeromonas hydrophila Didanosine Microbial transglycosylation Ribavirin Whole cell biocatalysts Amides Biomolecules Enzymes didanosine ribavirin Aeromonas hydrophila article biotransformation drug isolation drug purification drug structure drug synthesis glycosylation high performance liquid chromatography nonhuman Aeromonas hydrophila Microbial transglycosylation is useful as a green alternative in the preparation of purine nucleosides and analogues, especially for those that display pharmacological activities. In a search for new transglycosylation biocatalysts, two Aeromonas hydrophila strains were selected. The substrate specificity of both micro-organisms was studied and, as a result, several nucleoside analogues have been prepared. Among them, ribavirin, a broad spectrum antiviral, and the well-known anti HIV didanosine, were prepared, in 77 and 62% yield using A. hydrophila CECT 4226 and A. hydrophila CECT 4221, respectively. In order to scale-up the processes, the reaction conditions, product purification and biocatalyst preparation were analyzed and optimized. © 2010 Informa UK, Ltd. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10242422_v28_n5-6_p395_Nbile http://hdl.handle.net/20.500.12110/paper_10242422_v28_n5-6_p395_Nbile |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Aeromonas hydrophila Didanosine Microbial transglycosylation Ribavirin Whole cell biocatalysts Aeromonas hydrophila Didanosine Microbial transglycosylation Ribavirin Whole cell biocatalysts Amides Biomolecules Enzymes didanosine ribavirin Aeromonas hydrophila article biotransformation drug isolation drug purification drug structure drug synthesis glycosylation high performance liquid chromatography nonhuman Aeromonas hydrophila |
| spellingShingle |
Aeromonas hydrophila Didanosine Microbial transglycosylation Ribavirin Whole cell biocatalysts Aeromonas hydrophila Didanosine Microbial transglycosylation Ribavirin Whole cell biocatalysts Amides Biomolecules Enzymes didanosine ribavirin Aeromonas hydrophila article biotransformation drug isolation drug purification drug structure drug synthesis glycosylation high performance liquid chromatography nonhuman Aeromonas hydrophila Aeromonas hydrophila strains as biocatalysts for transglycosylation |
| topic_facet |
Aeromonas hydrophila Didanosine Microbial transglycosylation Ribavirin Whole cell biocatalysts Aeromonas hydrophila Didanosine Microbial transglycosylation Ribavirin Whole cell biocatalysts Amides Biomolecules Enzymes didanosine ribavirin Aeromonas hydrophila article biotransformation drug isolation drug purification drug structure drug synthesis glycosylation high performance liquid chromatography nonhuman Aeromonas hydrophila |
| description |
Microbial transglycosylation is useful as a green alternative in the preparation of purine nucleosides and analogues, especially for those that display pharmacological activities. In a search for new transglycosylation biocatalysts, two Aeromonas hydrophila strains were selected. The substrate specificity of both micro-organisms was studied and, as a result, several nucleoside analogues have been prepared. Among them, ribavirin, a broad spectrum antiviral, and the well-known anti HIV didanosine, were prepared, in 77 and 62% yield using A. hydrophila CECT 4226 and A. hydrophila CECT 4221, respectively. In order to scale-up the processes, the reaction conditions, product purification and biocatalyst preparation were analyzed and optimized. © 2010 Informa UK, Ltd. |
| title |
Aeromonas hydrophila strains as biocatalysts for transglycosylation |
| title_short |
Aeromonas hydrophila strains as biocatalysts for transglycosylation |
| title_full |
Aeromonas hydrophila strains as biocatalysts for transglycosylation |
| title_fullStr |
Aeromonas hydrophila strains as biocatalysts for transglycosylation |
| title_full_unstemmed |
Aeromonas hydrophila strains as biocatalysts for transglycosylation |
| title_sort |
aeromonas hydrophila strains as biocatalysts for transglycosylation |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10242422_v28_n5-6_p395_Nbile http://hdl.handle.net/20.500.12110/paper_10242422_v28_n5-6_p395_Nbile |
| _version_ |
1768543045259624448 |