Human periprostatic adipose tissue: Its influence on prostate cancer cells

Background/Aims: Adipose microenvironment is involved in signaling pathways that influence prostate cancer (PCa) progression. However, the role of human periprostatic adipose tissue (PPAT) from patients with benign prostatic hyperplasia (BPH) has not been studied and compared to that of PPAT from PC...

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Autores principales:	Sacca, Paula Alejandra, Pistone Creydt, Virginia, Fletcher, Sabrina Johanna, Calvo, Juan Carlos
Publicado:	2012
Materias:	Adipose tissue Metalloproteinases Microenvironment Periprostatic adipose tissue Periprostatic fat Prostate cancer androgen gelatinase B adipose tissue adult aged article cancer cell cancer surgery castration resistant prostate cancer cell adhesion cell migration cell motility cell proliferation cell strain LNCaP controlled study enzyme activity human human cell human tissue male periprostatic adipose tissue priority journal prostate hypertrophy prostatectomy protein expression zymography Aged Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Cell Transformation, Neoplastic Culture Media, Conditioned Humans Intra-Abdominal Fat Male Matrix Metalloproteinase 9 Middle Aged Neoplasms, Hormone-Dependent Prostate Prostatic Hyperplasia Prostatic Neoplasms Tumor Microenvironment
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10158987_v30_n1_p113_Sacca http://hdl.handle.net/20.500.12110/paper_10158987_v30_n1_p113_Sacca
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10158987_v30_n1_p113_Sacca
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spelling	paper:paper_10158987_v30_n1_p113_Sacca2023-06-08T15:59:47Z Human periprostatic adipose tissue: Its influence on prostate cancer cells Sacca, Paula Alejandra Pistone Creydt, Virginia Fletcher, Sabrina Johanna Calvo, Juan Carlos Adipose tissue Metalloproteinases Microenvironment Periprostatic adipose tissue Periprostatic fat Prostate cancer androgen gelatinase B adipose tissue adult aged article cancer cell cancer surgery castration resistant prostate cancer cell adhesion cell migration cell motility cell proliferation cell strain LNCaP controlled study enzyme activity human human cell human tissue male periprostatic adipose tissue priority journal prostate hypertrophy prostatectomy protein expression zymography Aged Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Cell Transformation, Neoplastic Culture Media, Conditioned Humans Intra-Abdominal Fat Male Matrix Metalloproteinase 9 Middle Aged Neoplasms, Hormone-Dependent Prostate Prostatic Hyperplasia Prostatic Neoplasms Tumor Microenvironment Background/Aims: Adipose microenvironment is involved in signaling pathways that influence prostate cancer (PCa) progression. However, the role of human periprostatic adipose tissue (PPAT) from patients with benign prostatic hyperplasia (BPH) has not been studied and compared to that of PPAT from PCa patients. The aim of this paper was to investigate the influence of factors derived from both PPATs on the behavior of androgen-dependent and castration resistant PCa cells. Methods: PPAT conditioned media (CM) were obtained from tissue samples from patients with clinically primary PCa (TPPAT) or BPH (BPPAT). Cell adhesion, proliferation, migration and metalloproteinase expression were evaluated following exposure of LNCaP (androgen dependent) and PC3 (androgen independent) prostate cancer cell lines to BPPAT or TPPAT CM. Results: Proliferation or motility of LNCaP or PC3 cells were not significantly affected by TPPAT or BPPAT CM. The number of LNCaP but not PC3 cells attached to components of TPPAT CM significantly decreased compared to cells attached to BPPAT CM. PPAT produced and released pro-MMP-9. Zymograms demonstrated that TPPAT CM induced a significant increase in pro-MMP-9 activity compared to BPPAT CM in LNCaP cells but not in PC3 cells. Conclusions: We conclude that TPPAT released factors, such as pro-MMP-9, could induce the invasive capacity of LNCaP cells and speculate that PPAT derived factors could, in the early stages of prostate cancer, modulate disease progression. © 2012 S. Karger AG, Basel. Fil:Sacca, P.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Creydt, V.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fletcher, S.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10158987_v30_n1_p113_Sacca http://hdl.handle.net/20.500.12110/paper_10158987_v30_n1_p113_Sacca
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adipose tissue Metalloproteinases Microenvironment Periprostatic adipose tissue Periprostatic fat Prostate cancer androgen gelatinase B adipose tissue adult aged article cancer cell cancer surgery castration resistant prostate cancer cell adhesion cell migration cell motility cell proliferation cell strain LNCaP controlled study enzyme activity human human cell human tissue male periprostatic adipose tissue priority journal prostate hypertrophy prostatectomy protein expression zymography Aged Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Cell Transformation, Neoplastic Culture Media, Conditioned Humans Intra-Abdominal Fat Male Matrix Metalloproteinase 9 Middle Aged Neoplasms, Hormone-Dependent Prostate Prostatic Hyperplasia Prostatic Neoplasms Tumor Microenvironment
spellingShingle	Adipose tissue Metalloproteinases Microenvironment Periprostatic adipose tissue Periprostatic fat Prostate cancer androgen gelatinase B adipose tissue adult aged article cancer cell cancer surgery castration resistant prostate cancer cell adhesion cell migration cell motility cell proliferation cell strain LNCaP controlled study enzyme activity human human cell human tissue male periprostatic adipose tissue priority journal prostate hypertrophy prostatectomy protein expression zymography Aged Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Cell Transformation, Neoplastic Culture Media, Conditioned Humans Intra-Abdominal Fat Male Matrix Metalloproteinase 9 Middle Aged Neoplasms, Hormone-Dependent Prostate Prostatic Hyperplasia Prostatic Neoplasms Tumor Microenvironment Sacca, Paula Alejandra Pistone Creydt, Virginia Fletcher, Sabrina Johanna Calvo, Juan Carlos Human periprostatic adipose tissue: Its influence on prostate cancer cells
topic_facet	Adipose tissue Metalloproteinases Microenvironment Periprostatic adipose tissue Periprostatic fat Prostate cancer androgen gelatinase B adipose tissue adult aged article cancer cell cancer surgery castration resistant prostate cancer cell adhesion cell migration cell motility cell proliferation cell strain LNCaP controlled study enzyme activity human human cell human tissue male periprostatic adipose tissue priority journal prostate hypertrophy prostatectomy protein expression zymography Aged Cell Adhesion Cell Line, Tumor Cell Movement Cell Proliferation Cell Transformation, Neoplastic Culture Media, Conditioned Humans Intra-Abdominal Fat Male Matrix Metalloproteinase 9 Middle Aged Neoplasms, Hormone-Dependent Prostate Prostatic Hyperplasia Prostatic Neoplasms Tumor Microenvironment
description	Background/Aims: Adipose microenvironment is involved in signaling pathways that influence prostate cancer (PCa) progression. However, the role of human periprostatic adipose tissue (PPAT) from patients with benign prostatic hyperplasia (BPH) has not been studied and compared to that of PPAT from PCa patients. The aim of this paper was to investigate the influence of factors derived from both PPATs on the behavior of androgen-dependent and castration resistant PCa cells. Methods: PPAT conditioned media (CM) were obtained from tissue samples from patients with clinically primary PCa (TPPAT) or BPH (BPPAT). Cell adhesion, proliferation, migration and metalloproteinase expression were evaluated following exposure of LNCaP (androgen dependent) and PC3 (androgen independent) prostate cancer cell lines to BPPAT or TPPAT CM. Results: Proliferation or motility of LNCaP or PC3 cells were not significantly affected by TPPAT or BPPAT CM. The number of LNCaP but not PC3 cells attached to components of TPPAT CM significantly decreased compared to cells attached to BPPAT CM. PPAT produced and released pro-MMP-9. Zymograms demonstrated that TPPAT CM induced a significant increase in pro-MMP-9 activity compared to BPPAT CM in LNCaP cells but not in PC3 cells. Conclusions: We conclude that TPPAT released factors, such as pro-MMP-9, could induce the invasive capacity of LNCaP cells and speculate that PPAT derived factors could, in the early stages of prostate cancer, modulate disease progression. © 2012 S. Karger AG, Basel.
author	Sacca, Paula Alejandra Pistone Creydt, Virginia Fletcher, Sabrina Johanna Calvo, Juan Carlos
author_facet	Sacca, Paula Alejandra Pistone Creydt, Virginia Fletcher, Sabrina Johanna Calvo, Juan Carlos
author_sort	Sacca, Paula Alejandra
title	Human periprostatic adipose tissue: Its influence on prostate cancer cells
title_short	Human periprostatic adipose tissue: Its influence on prostate cancer cells
title_full	Human periprostatic adipose tissue: Its influence on prostate cancer cells
title_fullStr	Human periprostatic adipose tissue: Its influence on prostate cancer cells
title_full_unstemmed	Human periprostatic adipose tissue: Its influence on prostate cancer cells
title_sort	human periprostatic adipose tissue: its influence on prostate cancer cells
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10158987_v30_n1_p113_Sacca http://hdl.handle.net/20.500.12110/paper_10158987_v30_n1_p113_Sacca
work_keys_str_mv	AT saccapaulaalejandra humanperiprostaticadiposetissueitsinfluenceonprostatecancercells AT pistonecreydtvirginia humanperiprostaticadiposetissueitsinfluenceonprostatecancercells AT fletchersabrinajohanna humanperiprostaticadiposetissueitsinfluenceonprostatecancercells AT calvojuancarlos humanperiprostaticadiposetissueitsinfluenceonprostatecancercells
_version_	1768544190526914560

Human periprostatic adipose tissue: Its influence on prostate cancer cells

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