Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity
In this work, we studied the in vitro interactions between aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) and nitric oxide (NO), as well as the interactions between ALA, porphyrins and some NO donors and precursors. We employed three murine adenocarcinoma cell lines: LM2, which does n...
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2005
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v80_n3_p195_DiVenosa http://hdl.handle.net/20.500.12110/paper_10111344_v80_n3_p195_DiVenosa |
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paper:paper_10111344_v80_n3_p195_DiVenosa2023-06-08T15:59:41Z Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity Di Venosa, Gabriela Mariana Perotti, Christian Pablo Fukuda, Haydeé Batlle, Alcira María del Carmen Casas, Adriana Gabriela 5-Aminolevulinic acid ALA-PDT Nitric oxide Photodynamic therapy aminolevulinic acid arginine hydrolyase nitric oxide nitric oxide donor nitroprusside sodium adenocarcinoma animal cell animal experiment animal model article biosynthesis blood flow cancer therapy carcinoma cell cell protection controlled study cross resistance cytotoxicity drug mechanism drug response enzyme inhibition hypothesis in vitro study in vivo study molecular interaction mouse nonhuman photodynamic therapy priority journal sensitivity analysis Adenocarcinoma Aminolevulinic Acid Animals Cell Line Cell Line, Tumor Cell Survival Female L Cells (Cell Line) Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Nitric Oxide Nitric Oxide Donors Photochemotherapy Porphyrins Murinae In this work, we studied the in vitro interactions between aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) and nitric oxide (NO), as well as the interactions between ALA, porphyrins and some NO donors and precursors. We employed three murine adenocarcinoma cell lines: LM2, which does not produce NO; LM3, which produces NO, and LM3-SNP, a variant of LM3 resistant to NO producing the same amount of NO as the parental. We did not find cross-resistance between NO-induced cytotoxicity and ALA-PDT. In spite of the lower porphyrin synthesis, LM2 cells show the highest sensitivity to ALA-PDT. However, we hypothesised that this is not related to the lack of endogenous NO production, because modulation of NO levels did not modify the response to PDT in any of the cell lines. Two unexpected results were found: the enhancement of NO production from the donor sodium nitroprusside (SNP) induced by ALA in both cells and medium, and the inhibition by ALA of NO production from arginine. We also found that SNP strongly protected the cells from ALA-PDT by impairing porphyrin biosynthesis as a consequence of an inhibition of the enzyme ALA dehydratase. We were not able to evaluate the action of NO derived from SNP because of the unexpected porphyrin impairment. On the other hand, impairment of NO from Arginine driven by ALA, although not modulating in vitro the ALA-PDT response, by increasing in vivo blood flow, may be contributing to the mechanism of tumour cures. © 2005 Elsevier B.V. All rights reserved. Fil:Di Venosa, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Perotti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v80_n3_p195_DiVenosa http://hdl.handle.net/20.500.12110/paper_10111344_v80_n3_p195_DiVenosa |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
5-Aminolevulinic acid ALA-PDT Nitric oxide Photodynamic therapy aminolevulinic acid arginine hydrolyase nitric oxide nitric oxide donor nitroprusside sodium adenocarcinoma animal cell animal experiment animal model article biosynthesis blood flow cancer therapy carcinoma cell cell protection controlled study cross resistance cytotoxicity drug mechanism drug response enzyme inhibition hypothesis in vitro study in vivo study molecular interaction mouse nonhuman photodynamic therapy priority journal sensitivity analysis Adenocarcinoma Aminolevulinic Acid Animals Cell Line Cell Line, Tumor Cell Survival Female L Cells (Cell Line) Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Nitric Oxide Nitric Oxide Donors Photochemotherapy Porphyrins Murinae |
spellingShingle |
5-Aminolevulinic acid ALA-PDT Nitric oxide Photodynamic therapy aminolevulinic acid arginine hydrolyase nitric oxide nitric oxide donor nitroprusside sodium adenocarcinoma animal cell animal experiment animal model article biosynthesis blood flow cancer therapy carcinoma cell cell protection controlled study cross resistance cytotoxicity drug mechanism drug response enzyme inhibition hypothesis in vitro study in vivo study molecular interaction mouse nonhuman photodynamic therapy priority journal sensitivity analysis Adenocarcinoma Aminolevulinic Acid Animals Cell Line Cell Line, Tumor Cell Survival Female L Cells (Cell Line) Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Nitric Oxide Nitric Oxide Donors Photochemotherapy Porphyrins Murinae Di Venosa, Gabriela Mariana Perotti, Christian Pablo Fukuda, Haydeé Batlle, Alcira María del Carmen Casas, Adriana Gabriela Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity |
topic_facet |
5-Aminolevulinic acid ALA-PDT Nitric oxide Photodynamic therapy aminolevulinic acid arginine hydrolyase nitric oxide nitric oxide donor nitroprusside sodium adenocarcinoma animal cell animal experiment animal model article biosynthesis blood flow cancer therapy carcinoma cell cell protection controlled study cross resistance cytotoxicity drug mechanism drug response enzyme inhibition hypothesis in vitro study in vivo study molecular interaction mouse nonhuman photodynamic therapy priority journal sensitivity analysis Adenocarcinoma Aminolevulinic Acid Animals Cell Line Cell Line, Tumor Cell Survival Female L Cells (Cell Line) Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Nitric Oxide Nitric Oxide Donors Photochemotherapy Porphyrins Murinae |
description |
In this work, we studied the in vitro interactions between aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) and nitric oxide (NO), as well as the interactions between ALA, porphyrins and some NO donors and precursors. We employed three murine adenocarcinoma cell lines: LM2, which does not produce NO; LM3, which produces NO, and LM3-SNP, a variant of LM3 resistant to NO producing the same amount of NO as the parental. We did not find cross-resistance between NO-induced cytotoxicity and ALA-PDT. In spite of the lower porphyrin synthesis, LM2 cells show the highest sensitivity to ALA-PDT. However, we hypothesised that this is not related to the lack of endogenous NO production, because modulation of NO levels did not modify the response to PDT in any of the cell lines. Two unexpected results were found: the enhancement of NO production from the donor sodium nitroprusside (SNP) induced by ALA in both cells and medium, and the inhibition by ALA of NO production from arginine. We also found that SNP strongly protected the cells from ALA-PDT by impairing porphyrin biosynthesis as a consequence of an inhibition of the enzyme ALA dehydratase. We were not able to evaluate the action of NO derived from SNP because of the unexpected porphyrin impairment. On the other hand, impairment of NO from Arginine driven by ALA, although not modulating in vitro the ALA-PDT response, by increasing in vivo blood flow, may be contributing to the mechanism of tumour cures. © 2005 Elsevier B.V. All rights reserved. |
author |
Di Venosa, Gabriela Mariana Perotti, Christian Pablo Fukuda, Haydeé Batlle, Alcira María del Carmen Casas, Adriana Gabriela |
author_facet |
Di Venosa, Gabriela Mariana Perotti, Christian Pablo Fukuda, Haydeé Batlle, Alcira María del Carmen Casas, Adriana Gabriela |
author_sort |
Di Venosa, Gabriela Mariana |
title |
Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity |
title_short |
Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity |
title_full |
Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity |
title_fullStr |
Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity |
title_full_unstemmed |
Sensitivity to ALA-PDT of cell lines with different nitric oxide production and resistance to NO cytotoxicity |
title_sort |
sensitivity to ala-pdt of cell lines with different nitric oxide production and resistance to no cytotoxicity |
publishDate |
2005 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v80_n3_p195_DiVenosa http://hdl.handle.net/20.500.12110/paper_10111344_v80_n3_p195_DiVenosa |
work_keys_str_mv |
AT divenosagabrielamariana sensitivitytoalapdtofcelllineswithdifferentnitricoxideproductionandresistancetonocytotoxicity AT perottichristianpablo sensitivitytoalapdtofcelllineswithdifferentnitricoxideproductionandresistancetonocytotoxicity AT fukudahaydee sensitivitytoalapdtofcelllineswithdifferentnitricoxideproductionandresistancetonocytotoxicity AT batllealciramariadelcarmen sensitivitytoalapdtofcelllineswithdifferentnitricoxideproductionandresistancetonocytotoxicity AT casasadrianagabriela sensitivitytoalapdtofcelllineswithdifferentnitricoxideproductionandresistancetonocytotoxicity |
_version_ |
1768541905430249472 |