A method for separating ALA from ALA derivatives using ionic exchange extraction

Photodynamic therapy using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX is a recent approach to detect and treat some malignancies. The use of lipophilic derivatives of ALA has been exploited in the last years to enhance ALA penetration. In this paper, we describe the application of the Mau...

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Autores principales:	Di Venosa, Gabriela Mariana, Fukuda, Haydeé, Perotti, Christian Pablo, Batlle, Alcira María del Carmen, Casas, Adriana Gabriela
Publicado:	2004
Materias:	ALA ALA derivatives ALA esters Aminolevulinic acid Dowex resin PDT Photodynamic therapy acetic acid amino acid derivative aminolevulinic acid hydrochloric acid hydrogen resin animal cell article concentration (parameters) controlled study elution extraction ion exchange light absorption nonhuman priority journal product recovery separation technique Aminolevulinic Acid Animals Chromatography, Ion Exchange Mice Tumor Cells, Cultured
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v75_n3_p157_DiVenosa http://hdl.handle.net/20.500.12110/paper_10111344_v75_n3_p157_DiVenosa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_10111344_v75_n3_p157_DiVenosa
record_format	dspace
spelling	paper:paper_10111344_v75_n3_p157_DiVenosa2023-06-08T15:59:41Z A method for separating ALA from ALA derivatives using ionic exchange extraction Di Venosa, Gabriela Mariana Fukuda, Haydeé Perotti, Christian Pablo Batlle, Alcira María del Carmen Casas, Adriana Gabriela ALA ALA derivatives ALA esters Aminolevulinic acid Dowex resin PDT Photodynamic therapy acetic acid amino acid derivative aminolevulinic acid hydrochloric acid hydrogen resin animal cell article concentration (parameters) controlled study elution extraction ion exchange light absorption nonhuman priority journal product recovery separation technique Aminolevulinic Acid Animals Chromatography, Ion Exchange Mice Tumor Cells, Cultured Photodynamic therapy using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX is a recent approach to detect and treat some malignancies. The use of lipophilic derivatives of ALA has been exploited in the last years to enhance ALA penetration. In this paper, we describe the application of the Mauzerall and Granick's method [J. Biol. Chem. 219 (1956) 435] to the quantification of ALA derivatives. We also describe the employment of reusable ion-exchange chromatographic columns for separating mixtures of ALA and ALA derivatives present in biological samples. The relation between 555 nm absorbance and ALA or ALA derivative concentration was linear up to 100 nmol/ml and the limit of detection of ALA and ALA derivatives was 1 nmol per ml. We employed a Dowex 50 X8 hydrogen form resin to separate ALA from the derivatives. Whereas 90 ± 4% of the total ALA was eluted using sodium acetate, only 3-9% of the ALA derivatives was recovered. Only upon exposure of the resin to a high HCl concentration, the ALA derivatives were completely released. We employed this new method for the separation of ALA from ALA derivatives in cells exposed to different ALA compounds. © 2004 Elsevier B.V. All rights reserved. Fil:Di Venosa, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fukuda, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Perotti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v75_n3_p157_DiVenosa http://hdl.handle.net/20.500.12110/paper_10111344_v75_n3_p157_DiVenosa
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	ALA ALA derivatives ALA esters Aminolevulinic acid Dowex resin PDT Photodynamic therapy acetic acid amino acid derivative aminolevulinic acid hydrochloric acid hydrogen resin animal cell article concentration (parameters) controlled study elution extraction ion exchange light absorption nonhuman priority journal product recovery separation technique Aminolevulinic Acid Animals Chromatography, Ion Exchange Mice Tumor Cells, Cultured
spellingShingle	ALA ALA derivatives ALA esters Aminolevulinic acid Dowex resin PDT Photodynamic therapy acetic acid amino acid derivative aminolevulinic acid hydrochloric acid hydrogen resin animal cell article concentration (parameters) controlled study elution extraction ion exchange light absorption nonhuman priority journal product recovery separation technique Aminolevulinic Acid Animals Chromatography, Ion Exchange Mice Tumor Cells, Cultured Di Venosa, Gabriela Mariana Fukuda, Haydeé Perotti, Christian Pablo Batlle, Alcira María del Carmen Casas, Adriana Gabriela A method for separating ALA from ALA derivatives using ionic exchange extraction
topic_facet	ALA ALA derivatives ALA esters Aminolevulinic acid Dowex resin PDT Photodynamic therapy acetic acid amino acid derivative aminolevulinic acid hydrochloric acid hydrogen resin animal cell article concentration (parameters) controlled study elution extraction ion exchange light absorption nonhuman priority journal product recovery separation technique Aminolevulinic Acid Animals Chromatography, Ion Exchange Mice Tumor Cells, Cultured
description	Photodynamic therapy using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX is a recent approach to detect and treat some malignancies. The use of lipophilic derivatives of ALA has been exploited in the last years to enhance ALA penetration. In this paper, we describe the application of the Mauzerall and Granick's method [J. Biol. Chem. 219 (1956) 435] to the quantification of ALA derivatives. We also describe the employment of reusable ion-exchange chromatographic columns for separating mixtures of ALA and ALA derivatives present in biological samples. The relation between 555 nm absorbance and ALA or ALA derivative concentration was linear up to 100 nmol/ml and the limit of detection of ALA and ALA derivatives was 1 nmol per ml. We employed a Dowex 50 X8 hydrogen form resin to separate ALA from the derivatives. Whereas 90 ± 4% of the total ALA was eluted using sodium acetate, only 3-9% of the ALA derivatives was recovered. Only upon exposure of the resin to a high HCl concentration, the ALA derivatives were completely released. We employed this new method for the separation of ALA from ALA derivatives in cells exposed to different ALA compounds. © 2004 Elsevier B.V. All rights reserved.
author	Di Venosa, Gabriela Mariana Fukuda, Haydeé Perotti, Christian Pablo Batlle, Alcira María del Carmen Casas, Adriana Gabriela
author_facet	Di Venosa, Gabriela Mariana Fukuda, Haydeé Perotti, Christian Pablo Batlle, Alcira María del Carmen Casas, Adriana Gabriela
author_sort	Di Venosa, Gabriela Mariana
title	A method for separating ALA from ALA derivatives using ionic exchange extraction
title_short	A method for separating ALA from ALA derivatives using ionic exchange extraction
title_full	A method for separating ALA from ALA derivatives using ionic exchange extraction
title_fullStr	A method for separating ALA from ALA derivatives using ionic exchange extraction
title_full_unstemmed	A method for separating ALA from ALA derivatives using ionic exchange extraction
title_sort	method for separating ala from ala derivatives using ionic exchange extraction
publishDate	2004
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v75_n3_p157_DiVenosa http://hdl.handle.net/20.500.12110/paper_10111344_v75_n3_p157_DiVenosa
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A method for separating ALA from ALA derivatives using ionic exchange extraction

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