Photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups
Biofilm-associated diseases account for 80% of all infections in humans. Due to the emergence of antibiotic resistances, alternative therapies such as Photodynamic Inactivation (PDI) of microorganisms have emerged. Porphyrins with intrinsic positive charges have been proposed as successful photosens...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v161_n_p222_Mamone http://hdl.handle.net/20.500.12110/paper_10111344_v161_n_p222_Mamone |
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paper:paper_10111344_v161_n_p222_Mamone2023-06-08T15:59:39Z Photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups Mamone, Leandro A. Saenz, Daniel Alberto Batlle, Alcira María del Carmen Buzzola, Fernanda Roxana Casas, Adriana Gabriela Bacteria Biofilm Photodynamic inactivation Planktonic Porphyrin Staphylococcus aureus photosensitizing agent porphyrin biofilm chemistry confocal microscopy drug effects light physiology Pseudomonas aeruginosa radiation response scanning electron microscopy Staphylococcus aureus Biofilms Light Microscopy, Confocal Microscopy, Electron, Scanning Photosensitizing Agents Porphyrins Pseudomonas aeruginosa Staphylococcus aureus Biofilm-associated diseases account for 80% of all infections in humans. Due to the emergence of antibiotic resistances, alternative therapies such as Photodynamic Inactivation (PDI) of microorganisms have emerged. Porphyrins with intrinsic positive charges have been proposed as successful photosensitizers (PSs) against microorganisms. We have recently designed the new synthetic porphyrin 5,10,15,20-tetrakis[4-(3-N,N-dimethylammoniumpropoxy)phenyl]porphyrin (TAPP) containing four basic amine groups in the periphery of the tetrapyrrolic macrocycle, which can acquire positive charges at physiological pH, thus favouring the interaction with biomembranes. Illumination of planktonic cultures of Staphylococcus aureus at 180 J/cm2 in the presence of 2.5 μM TAPP induced complete bacteria eradication. For the TAPP-PDI treatment of S. aureus biofilms, higher light fluences and PS concentrations were needed. Employing 20 μM TAPP and 180 J/cm2, around 3-log CFU reduction were obtained. In order to determine the efficacy of TAPP-PDI on Gram-negative bacteria, we performed planktonic and biofilm assays employing Pseudomonas aeruginosa. Much higher TAPP doses as compared to S. aureus were needed to achieve planktonic bacteria photosensitization (3-log CFU reduction at 20 μM TAPP and 180 J/cm2). On the other hand, high concentrations of TAPP were nontoxic to P. aeruginosa growing on biofilms, and employing 30 μM TAPP and 180 J/cm2 we obtained 3-log CFU reduction. The main conclusion of the present work is that TAPP is a promising and efficient PS capable of promoting photodynamic killing of both Gram-negative and -positive in planktonic bacteria, though more effectively in the latter. In addition, TAPP-PDI induces similar photoinactivation rates in both bacteria types growing on biofilms, with lower dark toxicity in the Gram-negative one. © 2016 Elsevier B.V. Fil:Mamone, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sáenz, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buzzola, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v161_n_p222_Mamone http://hdl.handle.net/20.500.12110/paper_10111344_v161_n_p222_Mamone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacteria Biofilm Photodynamic inactivation Planktonic Porphyrin Staphylococcus aureus photosensitizing agent porphyrin biofilm chemistry confocal microscopy drug effects light physiology Pseudomonas aeruginosa radiation response scanning electron microscopy Staphylococcus aureus Biofilms Light Microscopy, Confocal Microscopy, Electron, Scanning Photosensitizing Agents Porphyrins Pseudomonas aeruginosa Staphylococcus aureus |
| spellingShingle |
Bacteria Biofilm Photodynamic inactivation Planktonic Porphyrin Staphylococcus aureus photosensitizing agent porphyrin biofilm chemistry confocal microscopy drug effects light physiology Pseudomonas aeruginosa radiation response scanning electron microscopy Staphylococcus aureus Biofilms Light Microscopy, Confocal Microscopy, Electron, Scanning Photosensitizing Agents Porphyrins Pseudomonas aeruginosa Staphylococcus aureus Mamone, Leandro A. Saenz, Daniel Alberto Batlle, Alcira María del Carmen Buzzola, Fernanda Roxana Casas, Adriana Gabriela Photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups |
| topic_facet |
Bacteria Biofilm Photodynamic inactivation Planktonic Porphyrin Staphylococcus aureus photosensitizing agent porphyrin biofilm chemistry confocal microscopy drug effects light physiology Pseudomonas aeruginosa radiation response scanning electron microscopy Staphylococcus aureus Biofilms Light Microscopy, Confocal Microscopy, Electron, Scanning Photosensitizing Agents Porphyrins Pseudomonas aeruginosa Staphylococcus aureus |
| description |
Biofilm-associated diseases account for 80% of all infections in humans. Due to the emergence of antibiotic resistances, alternative therapies such as Photodynamic Inactivation (PDI) of microorganisms have emerged. Porphyrins with intrinsic positive charges have been proposed as successful photosensitizers (PSs) against microorganisms. We have recently designed the new synthetic porphyrin 5,10,15,20-tetrakis[4-(3-N,N-dimethylammoniumpropoxy)phenyl]porphyrin (TAPP) containing four basic amine groups in the periphery of the tetrapyrrolic macrocycle, which can acquire positive charges at physiological pH, thus favouring the interaction with biomembranes. Illumination of planktonic cultures of Staphylococcus aureus at 180 J/cm2 in the presence of 2.5 μM TAPP induced complete bacteria eradication. For the TAPP-PDI treatment of S. aureus biofilms, higher light fluences and PS concentrations were needed. Employing 20 μM TAPP and 180 J/cm2, around 3-log CFU reduction were obtained. In order to determine the efficacy of TAPP-PDI on Gram-negative bacteria, we performed planktonic and biofilm assays employing Pseudomonas aeruginosa. Much higher TAPP doses as compared to S. aureus were needed to achieve planktonic bacteria photosensitization (3-log CFU reduction at 20 μM TAPP and 180 J/cm2). On the other hand, high concentrations of TAPP were nontoxic to P. aeruginosa growing on biofilms, and employing 30 μM TAPP and 180 J/cm2 we obtained 3-log CFU reduction. The main conclusion of the present work is that TAPP is a promising and efficient PS capable of promoting photodynamic killing of both Gram-negative and -positive in planktonic bacteria, though more effectively in the latter. In addition, TAPP-PDI induces similar photoinactivation rates in both bacteria types growing on biofilms, with lower dark toxicity in the Gram-negative one. © 2016 Elsevier B.V. |
| author |
Mamone, Leandro A. Saenz, Daniel Alberto Batlle, Alcira María del Carmen Buzzola, Fernanda Roxana Casas, Adriana Gabriela |
| author_facet |
Mamone, Leandro A. Saenz, Daniel Alberto Batlle, Alcira María del Carmen Buzzola, Fernanda Roxana Casas, Adriana Gabriela |
| author_sort |
Mamone, Leandro A. |
| title |
Photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups |
| title_short |
Photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups |
| title_full |
Photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups |
| title_fullStr |
Photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups |
| title_full_unstemmed |
Photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups |
| title_sort |
photodynamic inactivation of planktonic and biofilm growing bacteria mediated by a meso-substituted porphyrin bearing four basic amino groups |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v161_n_p222_Mamone http://hdl.handle.net/20.500.12110/paper_10111344_v161_n_p222_Mamone |
| work_keys_str_mv |
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1768546501974294528 |