Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine
It is known that β-carbolines are able to produce photosensitized damage in cell-free DNA, but there is little information on their effects on cellular DNA. Therefore, we have analyzed the DNA damage produced by harmine and 9-methyl-harmine under UVA irradiation in V79 cells, together with the assoc...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v132_n_p66_Vignoni http://hdl.handle.net/20.500.12110/paper_10111344_v132_n_p66_Vignoni |
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paper:paper_10111344_v132_n_p66_Vignoni2023-06-08T15:59:38Z Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine Erra Balsells, Rosa Cyclobutane pyrimidine photodimers Micronuclei Photosensitization Phototoxicity β-carbolines 8 hydroxyguanine 9 methyl harmine beta carboline derivative cell DNA harmine unclassified drug 8-hydroxyguanine DNA guanine harmine absorption animal cell article cell counting cell proliferation controlled study cytotoxicity DNA damage DNA modification gel electrophoresis micronucleus nonhuman pH phototoxicity priority journal ultraviolet A radiation analogs and derivatives animal chemistry CHO cell line Cricetulus DNA damage drug effects hamster radiation response toxicity ultraviolet radiation Animals CHO Cells Cricetinae Cricetulus DNA DNA Damage Guanine Harmine Hydrogen-Ion Concentration Ultraviolet Rays It is known that β-carbolines are able to produce photosensitized damage in cell-free DNA, but there is little information on their effects on cellular DNA. Therefore, we have analyzed the DNA damage produced by harmine and 9-methyl-harmine under UVA irradiation in V79 cells, together with the associated generation of micronuclei and photocytotoxicity. The results indicate that the most frequent photoproducts generated in the cellular DNA are modified purines such as 8-oxo-7,8-dihydroguanine. Only relatively few single-strand breaks were observed. CPDs were absent, although they were generated in cell-free DNA irradiated under the same conditions. The overall extent of DNA damage in the cells was considerably smaller than the one observed in cell free DNA. The generation of cellular DNA damage was associated with a significant generation of micronuclei and decreased cell proliferation. The data indicate that β-carbolines act as photosensitizers in mammalian cells. The spectrum of DNA modification, and therefore the mechanism of DNA damage generation, differs considerably from that observed with cell-free DNA. © 2014 Elsevier B.V. All rights reserved. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v132_n_p66_Vignoni http://hdl.handle.net/20.500.12110/paper_10111344_v132_n_p66_Vignoni |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cyclobutane pyrimidine photodimers Micronuclei Photosensitization Phototoxicity β-carbolines 8 hydroxyguanine 9 methyl harmine beta carboline derivative cell DNA harmine unclassified drug 8-hydroxyguanine DNA guanine harmine absorption animal cell article cell counting cell proliferation controlled study cytotoxicity DNA damage DNA modification gel electrophoresis micronucleus nonhuman pH phototoxicity priority journal ultraviolet A radiation analogs and derivatives animal chemistry CHO cell line Cricetulus DNA damage drug effects hamster radiation response toxicity ultraviolet radiation Animals CHO Cells Cricetinae Cricetulus DNA DNA Damage Guanine Harmine Hydrogen-Ion Concentration Ultraviolet Rays |
| spellingShingle |
Cyclobutane pyrimidine photodimers Micronuclei Photosensitization Phototoxicity β-carbolines 8 hydroxyguanine 9 methyl harmine beta carboline derivative cell DNA harmine unclassified drug 8-hydroxyguanine DNA guanine harmine absorption animal cell article cell counting cell proliferation controlled study cytotoxicity DNA damage DNA modification gel electrophoresis micronucleus nonhuman pH phototoxicity priority journal ultraviolet A radiation analogs and derivatives animal chemistry CHO cell line Cricetulus DNA damage drug effects hamster radiation response toxicity ultraviolet radiation Animals CHO Cells Cricetinae Cricetulus DNA DNA Damage Guanine Harmine Hydrogen-Ion Concentration Ultraviolet Rays Erra Balsells, Rosa Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine |
| topic_facet |
Cyclobutane pyrimidine photodimers Micronuclei Photosensitization Phototoxicity β-carbolines 8 hydroxyguanine 9 methyl harmine beta carboline derivative cell DNA harmine unclassified drug 8-hydroxyguanine DNA guanine harmine absorption animal cell article cell counting cell proliferation controlled study cytotoxicity DNA damage DNA modification gel electrophoresis micronucleus nonhuman pH phototoxicity priority journal ultraviolet A radiation analogs and derivatives animal chemistry CHO cell line Cricetulus DNA damage drug effects hamster radiation response toxicity ultraviolet radiation Animals CHO Cells Cricetinae Cricetulus DNA DNA Damage Guanine Harmine Hydrogen-Ion Concentration Ultraviolet Rays |
| description |
It is known that β-carbolines are able to produce photosensitized damage in cell-free DNA, but there is little information on their effects on cellular DNA. Therefore, we have analyzed the DNA damage produced by harmine and 9-methyl-harmine under UVA irradiation in V79 cells, together with the associated generation of micronuclei and photocytotoxicity. The results indicate that the most frequent photoproducts generated in the cellular DNA are modified purines such as 8-oxo-7,8-dihydroguanine. Only relatively few single-strand breaks were observed. CPDs were absent, although they were generated in cell-free DNA irradiated under the same conditions. The overall extent of DNA damage in the cells was considerably smaller than the one observed in cell free DNA. The generation of cellular DNA damage was associated with a significant generation of micronuclei and decreased cell proliferation. The data indicate that β-carbolines act as photosensitizers in mammalian cells. The spectrum of DNA modification, and therefore the mechanism of DNA damage generation, differs considerably from that observed with cell-free DNA. © 2014 Elsevier B.V. All rights reserved. |
| author |
Erra Balsells, Rosa |
| author_facet |
Erra Balsells, Rosa |
| author_sort |
Erra Balsells, Rosa |
| title |
Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine |
| title_short |
Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine |
| title_full |
Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine |
| title_fullStr |
Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine |
| title_full_unstemmed |
Intra- and extra-cellular DNA damage by harmine and 9-methyl-harmine |
| title_sort |
intra- and extra-cellular dna damage by harmine and 9-methyl-harmine |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10111344_v132_n_p66_Vignoni http://hdl.handle.net/20.500.12110/paper_10111344_v132_n_p66_Vignoni |
| work_keys_str_mv |
AT errabalsellsrosa intraandextracellulardnadamagebyharmineand9methylharmine |
| _version_ |
1768545937741840384 |