Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress
Soil alkalinity is one of the most serious agricultural problems limiting crop yields. The legume Lotus tenuis is an important forage acknowledged by its ability to naturally grow in alkaline soils. To gain insight into the molecular responses that are activated by alkalinity in L.tenuis plants, sub...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v82_n_p279_Paz http://hdl.handle.net/20.500.12110/paper_09819428_v82_n_p279_Paz |
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paper:paper_09819428_v82_n_p279_Paz2023-06-08T15:59:15Z Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress Menéndez, Ana Bernardina Adaptive response Alkaline stress Long-term response Lotus tenuis Root and shoot tissues Stress induced transcripts Suppressive subtractive hybridization antacid agent drug effects gene expression regulation genetics Lotus plant plant root subtractive hybridization Antacids Gene Expression Regulation, Plant Lotus Plant Roots Plant Shoots Subtractive Hybridization Techniques Soil alkalinity is one of the most serious agricultural problems limiting crop yields. The legume Lotus tenuis is an important forage acknowledged by its ability to naturally grow in alkaline soils. To gain insight into the molecular responses that are activated by alkalinity in L.tenuis plants, subtractive cDNA libraries were generated from leaves and roots of these plants. Total RNAs of non-stressed plants (pH 5.8; E.C. 1.2), and plants stressed by the addition of 10mM of NaHCO3 (pH 9.0; E.C. 1.9), were used as source of the driver and the tester samples, respectively. RNA samples were collected after 14 and 28 days of treatment. A total of 158 unigenes from leaves and 92 unigenes from roots were obtained and classified into 11 functional categories. Unigenes from these categories (4 for leaves and 8 for roots), that were related with nutrient metabolism and oxidative stress relief were selected, and their differential expression analyzed by qRT-PCR. These genes were found to be differentially expressed in a time dependent manner in L.tenuis during the alkaline stress application. Data generated from this study will contribute to the understanding of the general molecular mechanisms associated to plant tolerance under long-term alkaline stress in plants. © 2014 Elsevier Masson SAS. Fil:Menéndez, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v82_n_p279_Paz http://hdl.handle.net/20.500.12110/paper_09819428_v82_n_p279_Paz |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adaptive response Alkaline stress Long-term response Lotus tenuis Root and shoot tissues Stress induced transcripts Suppressive subtractive hybridization antacid agent drug effects gene expression regulation genetics Lotus plant plant root subtractive hybridization Antacids Gene Expression Regulation, Plant Lotus Plant Roots Plant Shoots Subtractive Hybridization Techniques |
| spellingShingle |
Adaptive response Alkaline stress Long-term response Lotus tenuis Root and shoot tissues Stress induced transcripts Suppressive subtractive hybridization antacid agent drug effects gene expression regulation genetics Lotus plant plant root subtractive hybridization Antacids Gene Expression Regulation, Plant Lotus Plant Roots Plant Shoots Subtractive Hybridization Techniques Menéndez, Ana Bernardina Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress |
| topic_facet |
Adaptive response Alkaline stress Long-term response Lotus tenuis Root and shoot tissues Stress induced transcripts Suppressive subtractive hybridization antacid agent drug effects gene expression regulation genetics Lotus plant plant root subtractive hybridization Antacids Gene Expression Regulation, Plant Lotus Plant Roots Plant Shoots Subtractive Hybridization Techniques |
| description |
Soil alkalinity is one of the most serious agricultural problems limiting crop yields. The legume Lotus tenuis is an important forage acknowledged by its ability to naturally grow in alkaline soils. To gain insight into the molecular responses that are activated by alkalinity in L.tenuis plants, subtractive cDNA libraries were generated from leaves and roots of these plants. Total RNAs of non-stressed plants (pH 5.8; E.C. 1.2), and plants stressed by the addition of 10mM of NaHCO3 (pH 9.0; E.C. 1.9), were used as source of the driver and the tester samples, respectively. RNA samples were collected after 14 and 28 days of treatment. A total of 158 unigenes from leaves and 92 unigenes from roots were obtained and classified into 11 functional categories. Unigenes from these categories (4 for leaves and 8 for roots), that were related with nutrient metabolism and oxidative stress relief were selected, and their differential expression analyzed by qRT-PCR. These genes were found to be differentially expressed in a time dependent manner in L.tenuis during the alkaline stress application. Data generated from this study will contribute to the understanding of the general molecular mechanisms associated to plant tolerance under long-term alkaline stress in plants. © 2014 Elsevier Masson SAS. |
| author |
Menéndez, Ana Bernardina |
| author_facet |
Menéndez, Ana Bernardina |
| author_sort |
Menéndez, Ana Bernardina |
| title |
Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress |
| title_short |
Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress |
| title_full |
Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress |
| title_fullStr |
Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress |
| title_full_unstemmed |
Identification of differentially expressed genes potentially involved in the tolerance of Lotus tenuis to long-term alkaline stress |
| title_sort |
identification of differentially expressed genes potentially involved in the tolerance of lotus tenuis to long-term alkaline stress |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09819428_v82_n_p279_Paz http://hdl.handle.net/20.500.12110/paper_09819428_v82_n_p279_Paz |
| work_keys_str_mv |
AT menendezanabernardina identificationofdifferentiallyexpressedgenespotentiallyinvolvedinthetoleranceoflotustenuistolongtermalkalinestress |
| _version_ |
1768543382346399744 |