Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants

The present report shows a partial biochemical characterization and life cycle expression of N-β-alanyldopamine hydrolase (Tan protein) in Ceratitis capitata and Drosophila melanogaster. This enzyme catalyzes the hydrolysis of N-β-alanyldopamine (NBAD), the main tanning precursor of insect brown cut...

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Autores principales:	Pérez, Martín Mariano, Quesada Allué, Luis Alberto
Publicado:	2011
Materias:	β-alanylderivatives N-β-alanyldopamine NBAD-hydrolase NBAD-synthase DNA binding protein Drosophila protein insect protein nonhistone protein tan protein, Drosophila animal article brain dermis Drosophila melanogaster enzymology gene expression regulation genetics growth, development and aging insect larva Mediterranean fruit fly metabolism molecular genetics nucleotide sequence phenotype Animals Base Sequence Brain Ceratitis capitata Chromosomal Proteins, Non-Histone Dermis DNA-Binding Proteins Drosophila Proteins Gene Expression Regulation, Developmental Insect Proteins Larva Molecular Sequence Data Phenotype Pupa Hexapoda
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09651748_v41_n9_p653_Perez http://hdl.handle.net/20.500.12110/paper_09651748_v41_n9_p653_Perez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09651748_v41_n9_p653_Perez
record_format	dspace
spelling	paper:paper_09651748_v41_n9_p653_Perez2023-06-08T15:58:39Z Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants Pérez, Martín Mariano Quesada Allué, Luis Alberto β-alanylderivatives N-β-alanyldopamine NBAD-hydrolase NBAD-synthase DNA binding protein Drosophila protein insect protein nonhistone protein tan protein, Drosophila animal article brain dermis Drosophila melanogaster enzymology gene expression regulation genetics growth, development and aging insect larva Mediterranean fruit fly metabolism molecular genetics nucleotide sequence phenotype Animals Base Sequence Brain Ceratitis capitata Chromosomal Proteins, Non-Histone Dermis DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Gene Expression Regulation, Developmental Insect Proteins Larva Molecular Sequence Data Phenotype Pupa Ceratitis capitata Drosophila melanogaster Hexapoda The present report shows a partial biochemical characterization and life cycle expression of N-β-alanyldopamine hydrolase (Tan protein) in Ceratitis capitata and Drosophila melanogaster. This enzyme catalyzes the hydrolysis of N-β-alanyldopamine (NBAD), the main tanning precursor of insect brown cuticles. It also plays an important role in the metabolism of brain neurotransmitters, recycling dopamine and histamine. In contrast to NBAD-synthase, Tan is expressed constitutively in epidermis and does not respond directly to microbial challenge. Immunodetection experiments showed the novel localization of NBAD-hydrolase in the embryo central neural system and in different regions of the adult brain, in addition to optic lobes. We sequenced and characterized Drosophila mutants tan1 and tan3. The latter appears to be a mutant with normal expression in neural tissue but weak one in epidermis. © 2011 Elsevier Ltd. Fil:Pérez, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Quesada-Allué, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09651748_v41_n9_p653_Perez http://hdl.handle.net/20.500.12110/paper_09651748_v41_n9_p653_Perez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	β-alanylderivatives N-β-alanyldopamine NBAD-hydrolase NBAD-synthase DNA binding protein Drosophila protein insect protein nonhistone protein tan protein, Drosophila animal article brain dermis Drosophila melanogaster enzymology gene expression regulation genetics growth, development and aging insect larva Mediterranean fruit fly metabolism molecular genetics nucleotide sequence phenotype Animals Base Sequence Brain Ceratitis capitata Chromosomal Proteins, Non-Histone Dermis DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Gene Expression Regulation, Developmental Insect Proteins Larva Molecular Sequence Data Phenotype Pupa Ceratitis capitata Drosophila melanogaster Hexapoda
spellingShingle	β-alanylderivatives N-β-alanyldopamine NBAD-hydrolase NBAD-synthase DNA binding protein Drosophila protein insect protein nonhistone protein tan protein, Drosophila animal article brain dermis Drosophila melanogaster enzymology gene expression regulation genetics growth, development and aging insect larva Mediterranean fruit fly metabolism molecular genetics nucleotide sequence phenotype Animals Base Sequence Brain Ceratitis capitata Chromosomal Proteins, Non-Histone Dermis DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Gene Expression Regulation, Developmental Insect Proteins Larva Molecular Sequence Data Phenotype Pupa Ceratitis capitata Drosophila melanogaster Hexapoda Pérez, Martín Mariano Quesada Allué, Luis Alberto Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants
topic_facet	β-alanylderivatives N-β-alanyldopamine NBAD-hydrolase NBAD-synthase DNA binding protein Drosophila protein insect protein nonhistone protein tan protein, Drosophila animal article brain dermis Drosophila melanogaster enzymology gene expression regulation genetics growth, development and aging insect larva Mediterranean fruit fly metabolism molecular genetics nucleotide sequence phenotype Animals Base Sequence Brain Ceratitis capitata Chromosomal Proteins, Non-Histone Dermis DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Gene Expression Regulation, Developmental Insect Proteins Larva Molecular Sequence Data Phenotype Pupa Ceratitis capitata Drosophila melanogaster Hexapoda
description	The present report shows a partial biochemical characterization and life cycle expression of N-β-alanyldopamine hydrolase (Tan protein) in Ceratitis capitata and Drosophila melanogaster. This enzyme catalyzes the hydrolysis of N-β-alanyldopamine (NBAD), the main tanning precursor of insect brown cuticles. It also plays an important role in the metabolism of brain neurotransmitters, recycling dopamine and histamine. In contrast to NBAD-synthase, Tan is expressed constitutively in epidermis and does not respond directly to microbial challenge. Immunodetection experiments showed the novel localization of NBAD-hydrolase in the embryo central neural system and in different regions of the adult brain, in addition to optic lobes. We sequenced and characterized Drosophila mutants tan1 and tan3. The latter appears to be a mutant with normal expression in neural tissue but weak one in epidermis. © 2011 Elsevier Ltd.
author	Pérez, Martín Mariano Quesada Allué, Luis Alberto
author_facet	Pérez, Martín Mariano Quesada Allué, Luis Alberto
author_sort	Pérez, Martín Mariano
title	Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants
title_short	Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants
title_full	Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants
title_fullStr	Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants
title_full_unstemmed	Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants
title_sort	constitutive expression and enzymatic activity of tan protein in brain and epidermis of ceratitis capitata and of drosophila melanogaster wild-type and tan mutants
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09651748_v41_n9_p653_Perez http://hdl.handle.net/20.500.12110/paper_09651748_v41_n9_p653_Perez
work_keys_str_mv	AT perezmartinmariano constitutiveexpressionandenzymaticactivityoftanproteininbrainandepidermisofceratitiscapitataandofdrosophilamelanogasterwildtypeandtanmutants AT quesadaallueluisalberto constitutiveexpressionandenzymaticactivityoftanproteininbrainandepidermisofceratitiscapitataandofdrosophilamelanogasterwildtypeandtanmutants
_version_	1768545426708889600

Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants

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