Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators

Trametes trogii BAFC 463 culture fluids (containing 110Uml-1laccase; 0.94Uml-1 manganese peroxidase), as well as its purified laccase were capable of decolorizing azoic, indigoid, triphenylmethane, anthraquinonic and heterocyclic dyes, in the absence of redox mediators. Six dyes: RBBR, Indigo Carmin...

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Autores principales:	Grassi, Emanuel Marcelo, Scodeller, Pablo David, Levin, Laura Noemi
Publicado:	2011
Materias:	Dye decolorization Laccase Manganese peroxidase Redox mediators White-rot fungi White rot fungi Cadmium Carbonate minerals Dyes Fluids Heavy metals Manganese Organic polymers Synthetic metals Purification biodegradation concentration (composition) dye effluent enzyme activity fungus pH pollutant removal purification redox conditions waste treatment Frasera Funalia trogii Fungi Trametes
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09648305_v65_n4_p635_Grassi http://hdl.handle.net/20.500.12110/paper_09648305_v65_n4_p635_Grassi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09648305_v65_n4_p635_Grassi
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spelling	paper:paper_09648305_v65_n4_p635_Grassi2023-06-08T15:58:36Z Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators Grassi, Emanuel Marcelo Scodeller, Pablo David Levin, Laura Noemi Dye decolorization Laccase Manganese peroxidase Redox mediators White-rot fungi Dye decolorization Laccase Manganese peroxidase Redox mediators White rot fungi Cadmium Carbonate minerals Dyes Fluids Heavy metals Manganese Organic polymers Synthetic metals Purification biodegradation concentration (composition) dye effluent enzyme activity fungus pH pollutant removal purification redox conditions waste treatment Frasera Funalia trogii Fungi Trametes Trametes trogii BAFC 463 culture fluids (containing 110Uml-1laccase; 0.94Uml-1 manganese peroxidase), as well as its purified laccase were capable of decolorizing azoic, indigoid, triphenylmethane, anthraquinonic and heterocyclic dyes, in the absence of redox mediators. Six dyes: RBBR, Indigo Carmine, Xylidine, Malachite Green, Gentian Violet and Bromophenol Blue were almost completely degraded (more than 85% decolorization after 1 d) by either laccase or T. trogii itself in culture, proving the role of the enzyme in dye decolorization. The purified laccase also decolorized 65% of Fast Blue RR and 30% of Azure B and Methylene Blue after 24h. The use of redox mediators significantly increased the decolorization rates (90% decolorization of Azure B after 1h). 1-hydroxybenzotriazole resulted the best redox mediator, but the natural mediator p-hydroxybenzoic acid also demonstrated its efficiency for dye decolorization. Due to their ability to decolorize recalcitrant dyes without addition of redox mediators, high laccase activities, high thermostability and efficient decolorization at 70°C and pH 7.0, even in the presence of high concentrations of heavy metals (100mM Cu+2, Pb+2 or Cd+2) or in a synthetic dyebath, T. trogii culture fluids could be effectively used to decolorize synthetic dyes from effluents. © 2011 Elsevier Ltd. Fil:Grassi, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scodeller, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09648305_v65_n4_p635_Grassi http://hdl.handle.net/20.500.12110/paper_09648305_v65_n4_p635_Grassi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Dye decolorization Laccase Manganese peroxidase Redox mediators White-rot fungi Dye decolorization Laccase Manganese peroxidase Redox mediators White rot fungi Cadmium Carbonate minerals Dyes Fluids Heavy metals Manganese Organic polymers Synthetic metals Purification biodegradation concentration (composition) dye effluent enzyme activity fungus pH pollutant removal purification redox conditions waste treatment Frasera Funalia trogii Fungi Trametes
spellingShingle	Dye decolorization Laccase Manganese peroxidase Redox mediators White-rot fungi Dye decolorization Laccase Manganese peroxidase Redox mediators White rot fungi Cadmium Carbonate minerals Dyes Fluids Heavy metals Manganese Organic polymers Synthetic metals Purification biodegradation concentration (composition) dye effluent enzyme activity fungus pH pollutant removal purification redox conditions waste treatment Frasera Funalia trogii Fungi Trametes Grassi, Emanuel Marcelo Scodeller, Pablo David Levin, Laura Noemi Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
topic_facet	Dye decolorization Laccase Manganese peroxidase Redox mediators White-rot fungi Dye decolorization Laccase Manganese peroxidase Redox mediators White rot fungi Cadmium Carbonate minerals Dyes Fluids Heavy metals Manganese Organic polymers Synthetic metals Purification biodegradation concentration (composition) dye effluent enzyme activity fungus pH pollutant removal purification redox conditions waste treatment Frasera Funalia trogii Fungi Trametes
description	Trametes trogii BAFC 463 culture fluids (containing 110Uml-1laccase; 0.94Uml-1 manganese peroxidase), as well as its purified laccase were capable of decolorizing azoic, indigoid, triphenylmethane, anthraquinonic and heterocyclic dyes, in the absence of redox mediators. Six dyes: RBBR, Indigo Carmine, Xylidine, Malachite Green, Gentian Violet and Bromophenol Blue were almost completely degraded (more than 85% decolorization after 1 d) by either laccase or T. trogii itself in culture, proving the role of the enzyme in dye decolorization. The purified laccase also decolorized 65% of Fast Blue RR and 30% of Azure B and Methylene Blue after 24h. The use of redox mediators significantly increased the decolorization rates (90% decolorization of Azure B after 1h). 1-hydroxybenzotriazole resulted the best redox mediator, but the natural mediator p-hydroxybenzoic acid also demonstrated its efficiency for dye decolorization. Due to their ability to decolorize recalcitrant dyes without addition of redox mediators, high laccase activities, high thermostability and efficient decolorization at 70°C and pH 7.0, even in the presence of high concentrations of heavy metals (100mM Cu+2, Pb+2 or Cd+2) or in a synthetic dyebath, T. trogii culture fluids could be effectively used to decolorize synthetic dyes from effluents. © 2011 Elsevier Ltd.
author	Grassi, Emanuel Marcelo Scodeller, Pablo David Levin, Laura Noemi
author_facet	Grassi, Emanuel Marcelo Scodeller, Pablo David Levin, Laura Noemi
author_sort	Grassi, Emanuel Marcelo
title	Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
title_short	Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
title_full	Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
title_fullStr	Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
title_full_unstemmed	Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
title_sort	potential of trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09648305_v65_n4_p635_Grassi http://hdl.handle.net/20.500.12110/paper_09648305_v65_n4_p635_Grassi
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Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators

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