Knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a quantitative trait locus region of chromosome 2 in Drosophila melanogaster
In insects, two ecologically relevant traits of thermal adaptation are knockdown resistance to high temperature (KRHT) and chill-coma recovery (CCR). Chromosome 2 of Drosophila melanogaster was tested for quantitative trait loci (QTL) affecting both CCR and KRHT in backcrosses between homosequential...
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2007
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paper:paper_09621083_v16_n15_p3274_Norry2023-06-08T15:58:00Z Knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a quantitative trait locus region of chromosome 2 in Drosophila melanogaster Norry, Fabian Marcelo Gomez, Federico Hernan Cold-stress resistance Drosophila Heat-stress resistance Hsp70 expression QTL Thermal adaptation Drosophila protein heat shock protein 70 adaptation animal article chromosome chromosome map cold Drosophila melanogaster genetics heat heat shock response male metabolism phenotype physiology quantitative trait locus Adaptation, Biological Animals Chromosome Mapping Chromosomes Cold Drosophila melanogaster Drosophila Proteins Heat Heat-Shock Response HSP70 Heat-Shock Proteins Male Phenotype Quantitative Trait Loci Drosophila melanogaster Hexapoda In insects, two ecologically relevant traits of thermal adaptation are knockdown resistance to high temperature (KRHT) and chill-coma recovery (CCR). Chromosome 2 of Drosophila melanogaster was tested for quantitative trait loci (QTL) affecting both CCR and KRHT in backcrosses between homosequential lines that are fixed for the standard (noninverted) sequence of this autosome. These lines were obtained by artificial selection on KRHT and subsequent inbreeding from a stock that was derived from a single wild population. Heat-induced expression of the 70KD heat-shock protein (Hsp70) was also examined for variation between the lines. Composite interval mapping was performed for each trait on each reciprocal backcross, identifying one QTL region in the middle of chromosome 2 for both KRHT and CCR. The largest estimates of additive effects were found in pericentromeric regions of chromosome 2, accounting for 10-14% (CCR) and 10-17% (KRHT) of the phenotypic variance in BC populations. No QTL was found in the region of the heat-shock factor (hsf) gene. However, the two parental lines have diverged in the heat-induced Hsp70 expression. Distribution of KRHT QTL on chromosome 2 was similar between this study based on crosses between lines selected from a single wild population and previous work based on crosses between selection lines from different continents. Colocalized QTL showed a trade-off association between CCR and KRHT, which should be the result of either multiple, tightly linked trait-specific genes or a single gene with pleiotropic effects on the traits. We discuss candidate loci contained within the QTL regions. © 2007 The Authors. Fil:Norry, F.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gomez, F.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09621083_v16_n15_p3274_Norry http://hdl.handle.net/20.500.12110/paper_09621083_v16_n15_p3274_Norry |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cold-stress resistance Drosophila Heat-stress resistance Hsp70 expression QTL Thermal adaptation Drosophila protein heat shock protein 70 adaptation animal article chromosome chromosome map cold Drosophila melanogaster genetics heat heat shock response male metabolism phenotype physiology quantitative trait locus Adaptation, Biological Animals Chromosome Mapping Chromosomes Cold Drosophila melanogaster Drosophila Proteins Heat Heat-Shock Response HSP70 Heat-Shock Proteins Male Phenotype Quantitative Trait Loci Drosophila melanogaster Hexapoda |
| spellingShingle |
Cold-stress resistance Drosophila Heat-stress resistance Hsp70 expression QTL Thermal adaptation Drosophila protein heat shock protein 70 adaptation animal article chromosome chromosome map cold Drosophila melanogaster genetics heat heat shock response male metabolism phenotype physiology quantitative trait locus Adaptation, Biological Animals Chromosome Mapping Chromosomes Cold Drosophila melanogaster Drosophila Proteins Heat Heat-Shock Response HSP70 Heat-Shock Proteins Male Phenotype Quantitative Trait Loci Drosophila melanogaster Hexapoda Norry, Fabian Marcelo Gomez, Federico Hernan Knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a quantitative trait locus region of chromosome 2 in Drosophila melanogaster |
| topic_facet |
Cold-stress resistance Drosophila Heat-stress resistance Hsp70 expression QTL Thermal adaptation Drosophila protein heat shock protein 70 adaptation animal article chromosome chromosome map cold Drosophila melanogaster genetics heat heat shock response male metabolism phenotype physiology quantitative trait locus Adaptation, Biological Animals Chromosome Mapping Chromosomes Cold Drosophila melanogaster Drosophila Proteins Heat Heat-Shock Response HSP70 Heat-Shock Proteins Male Phenotype Quantitative Trait Loci Drosophila melanogaster Hexapoda |
| description |
In insects, two ecologically relevant traits of thermal adaptation are knockdown resistance to high temperature (KRHT) and chill-coma recovery (CCR). Chromosome 2 of Drosophila melanogaster was tested for quantitative trait loci (QTL) affecting both CCR and KRHT in backcrosses between homosequential lines that are fixed for the standard (noninverted) sequence of this autosome. These lines were obtained by artificial selection on KRHT and subsequent inbreeding from a stock that was derived from a single wild population. Heat-induced expression of the 70KD heat-shock protein (Hsp70) was also examined for variation between the lines. Composite interval mapping was performed for each trait on each reciprocal backcross, identifying one QTL region in the middle of chromosome 2 for both KRHT and CCR. The largest estimates of additive effects were found in pericentromeric regions of chromosome 2, accounting for 10-14% (CCR) and 10-17% (KRHT) of the phenotypic variance in BC populations. No QTL was found in the region of the heat-shock factor (hsf) gene. However, the two parental lines have diverged in the heat-induced Hsp70 expression. Distribution of KRHT QTL on chromosome 2 was similar between this study based on crosses between lines selected from a single wild population and previous work based on crosses between selection lines from different continents. Colocalized QTL showed a trade-off association between CCR and KRHT, which should be the result of either multiple, tightly linked trait-specific genes or a single gene with pleiotropic effects on the traits. We discuss candidate loci contained within the QTL regions. © 2007 The Authors. |
| author |
Norry, Fabian Marcelo Gomez, Federico Hernan |
| author_facet |
Norry, Fabian Marcelo Gomez, Federico Hernan |
| author_sort |
Norry, Fabian Marcelo |
| title |
Knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a quantitative trait locus region of chromosome 2 in Drosophila melanogaster |
| title_short |
Knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a quantitative trait locus region of chromosome 2 in Drosophila melanogaster |
| title_full |
Knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a quantitative trait locus region of chromosome 2 in Drosophila melanogaster |
| title_fullStr |
Knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a quantitative trait locus region of chromosome 2 in Drosophila melanogaster |
| title_full_unstemmed |
Knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a quantitative trait locus region of chromosome 2 in Drosophila melanogaster |
| title_sort |
knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a quantitative trait locus region of chromosome 2 in drosophila melanogaster |
| publishDate |
2007 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09621083_v16_n15_p3274_Norry http://hdl.handle.net/20.500.12110/paper_09621083_v16_n15_p3274_Norry |
| work_keys_str_mv |
AT norryfabianmarcelo knockdownresistancetoheatstressandslowrecoveryfromchillcomaaregeneticallyassociatedinaquantitativetraitlocusregionofchromosome2indrosophilamelanogaster AT gomezfedericohernan knockdownresistancetoheatstressandslowrecoveryfromchillcomaaregeneticallyassociatedinaquantitativetraitlocusregionofchromosome2indrosophilamelanogaster |
| _version_ |
1768546261994045440 |