Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity
Bordetella bronchiseptica produces respiratory disease primarily in mammals including humans. Although a considerably amount of research has been generated regarding lipopolysaccharide (LPS) role during infection and stimulating innate and adaptive immune response, mechanisms involved in LPS synthes...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v27_n3_p432_Sisti http://hdl.handle.net/20.500.12110/paper_0960894X_v27_n3_p432_Sisti |
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paper:paper_0960894X_v27_n3_p432_Sisti2023-06-08T15:57:53Z Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity Bordetella Glycosyltransferase Lipopolysaccharide bacterium lipopolysaccharide glycosyltransferase bacterial protein lipopolysaccharide adaptive immunity animal experiment Article bacterial gene bacterial genome BB3394 gene BB3400 gene Bordetella bronchiseptica Bordetella bronchiseptica infection chemical modification controlled study deletion mutant gene locus genetic conservation immune response in vivo study innate immunity mouse nonhuman animal biosynthesis Bordetella bronchiseptica chemistry genetics immune system immunology matrix-assisted laser desorption-ionization mass spectrometry metabolism Animals Bacterial Proteins Bordetella bronchiseptica Genes, Bacterial Glycosyltransferases Immune System Lipopolysaccharides Mice Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Bordetella bronchiseptica produces respiratory disease primarily in mammals including humans. Although a considerably amount of research has been generated regarding lipopolysaccharide (LPS) role during infection and stimulating innate and adaptive immune response, mechanisms involved in LPS synthesis are still unknown. In this context we searched in B. bronchiseptica genome for putative glycosyltransferases. We found possible genes codifying for enzymes involved in sugar substitution of the LPS structure. We decided to analyse BB3394 to BB3400 genes, closed to a previously described LPS biosynthetic locus in B. pertussis. Particularly, conservation of BB3394 in sequenced B. bronchiseptica genomes suggests the importance of this gene for bacteria normal physiology. Deletion of BB3394 abolished resistance to naive serum as described for other LPS mutants. When purified LPS was analyzed, differences in the LPS core structure were found. Particularly, a GalNA branched sugar substitution in the core was absent in the LPS obtained from BB3394 deletion mutant. Absence of GalNA in core LPS alters immune response in vivo but is able to induce protective response against B. bronchiseptica infection. © 2016 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v27_n3_p432_Sisti http://hdl.handle.net/20.500.12110/paper_0960894X_v27_n3_p432_Sisti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bordetella Glycosyltransferase Lipopolysaccharide bacterium lipopolysaccharide glycosyltransferase bacterial protein lipopolysaccharide adaptive immunity animal experiment Article bacterial gene bacterial genome BB3394 gene BB3400 gene Bordetella bronchiseptica Bordetella bronchiseptica infection chemical modification controlled study deletion mutant gene locus genetic conservation immune response in vivo study innate immunity mouse nonhuman animal biosynthesis Bordetella bronchiseptica chemistry genetics immune system immunology matrix-assisted laser desorption-ionization mass spectrometry metabolism Animals Bacterial Proteins Bordetella bronchiseptica Genes, Bacterial Glycosyltransferases Immune System Lipopolysaccharides Mice Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| spellingShingle |
Bordetella Glycosyltransferase Lipopolysaccharide bacterium lipopolysaccharide glycosyltransferase bacterial protein lipopolysaccharide adaptive immunity animal experiment Article bacterial gene bacterial genome BB3394 gene BB3400 gene Bordetella bronchiseptica Bordetella bronchiseptica infection chemical modification controlled study deletion mutant gene locus genetic conservation immune response in vivo study innate immunity mouse nonhuman animal biosynthesis Bordetella bronchiseptica chemistry genetics immune system immunology matrix-assisted laser desorption-ionization mass spectrometry metabolism Animals Bacterial Proteins Bordetella bronchiseptica Genes, Bacterial Glycosyltransferases Immune System Lipopolysaccharides Mice Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity |
| topic_facet |
Bordetella Glycosyltransferase Lipopolysaccharide bacterium lipopolysaccharide glycosyltransferase bacterial protein lipopolysaccharide adaptive immunity animal experiment Article bacterial gene bacterial genome BB3394 gene BB3400 gene Bordetella bronchiseptica Bordetella bronchiseptica infection chemical modification controlled study deletion mutant gene locus genetic conservation immune response in vivo study innate immunity mouse nonhuman animal biosynthesis Bordetella bronchiseptica chemistry genetics immune system immunology matrix-assisted laser desorption-ionization mass spectrometry metabolism Animals Bacterial Proteins Bordetella bronchiseptica Genes, Bacterial Glycosyltransferases Immune System Lipopolysaccharides Mice Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| description |
Bordetella bronchiseptica produces respiratory disease primarily in mammals including humans. Although a considerably amount of research has been generated regarding lipopolysaccharide (LPS) role during infection and stimulating innate and adaptive immune response, mechanisms involved in LPS synthesis are still unknown. In this context we searched in B. bronchiseptica genome for putative glycosyltransferases. We found possible genes codifying for enzymes involved in sugar substitution of the LPS structure. We decided to analyse BB3394 to BB3400 genes, closed to a previously described LPS biosynthetic locus in B. pertussis. Particularly, conservation of BB3394 in sequenced B. bronchiseptica genomes suggests the importance of this gene for bacteria normal physiology. Deletion of BB3394 abolished resistance to naive serum as described for other LPS mutants. When purified LPS was analyzed, differences in the LPS core structure were found. Particularly, a GalNA branched sugar substitution in the core was absent in the LPS obtained from BB3394 deletion mutant. Absence of GalNA in core LPS alters immune response in vivo but is able to induce protective response against B. bronchiseptica infection. © 2016 |
| title |
Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity |
| title_short |
Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity |
| title_full |
Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity |
| title_fullStr |
Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity |
| title_full_unstemmed |
Modifications of Bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity |
| title_sort |
modifications of bordetella bronchiseptica core lipopolysaccharide influence immune response without affecting protective activity |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v27_n3_p432_Sisti http://hdl.handle.net/20.500.12110/paper_0960894X_v27_n3_p432_Sisti |
| _version_ |
1768543191419584512 |