10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core
The catalytic core of a 10-23 DNAzyme was modified using (2′R), (2′S)-2′-deoxy-2′-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg 2+ concentrations, indicating that certain 2′-C-methyl modified DNAzymes have significant activities. Re...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v20_n15_p4367_Robaldo http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n15_p4367_Robaldo |
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| Sumario: | The catalytic core of a 10-23 DNAzyme was modified using (2′R), (2′S)-2′-deoxy-2′-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg 2+ concentrations, indicating that certain 2′-C-methyl modified DNAzymes have significant activities. Resistance against MCF-7 cell lysate and endonuclease RQ1 was also measured, showing that the introduction of 2′-C-methyl-2′-deoxynucleosides increased the stability. © 2010 Elsevier Ltd. All rights reserved. |
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