10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core
The catalytic core of a 10-23 DNAzyme was modified using (2′R), (2′S)-2′-deoxy-2′-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg 2+ concentrations, indicating that certain 2′-C-methyl modified DNAzymes have significant activities. Re...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v20_n15_p4367_Robaldo http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n15_p4367_Robaldo |
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paper:paper_0960894X_v20_n15_p4367_Robaldo2023-06-08T15:57:51Z 10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core 10-23 DNAzyme 2′-Deoxy-2′-C-methyl nucleosides 10 23 DNAzyme 2' deoxy 2' c methyluridine deoxyribozyme endonuclease endonuclease RQ1 magnesium ion nucleoside analog unclassified drug uridine derivative article cancer cell culture cancer resistance catalysis cell lysate controlled study DNA modification drug synthesis enzyme activity human human cell Catalytic Domain Cell Line, Tumor DNA, Catalytic DNA, Single-Stranded Humans Magnesium Uridine The catalytic core of a 10-23 DNAzyme was modified using (2′R), (2′S)-2′-deoxy-2′-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg 2+ concentrations, indicating that certain 2′-C-methyl modified DNAzymes have significant activities. Resistance against MCF-7 cell lysate and endonuclease RQ1 was also measured, showing that the introduction of 2′-C-methyl-2′-deoxynucleosides increased the stability. © 2010 Elsevier Ltd. All rights reserved. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v20_n15_p4367_Robaldo http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n15_p4367_Robaldo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
10-23 DNAzyme 2′-Deoxy-2′-C-methyl nucleosides 10 23 DNAzyme 2' deoxy 2' c methyluridine deoxyribozyme endonuclease endonuclease RQ1 magnesium ion nucleoside analog unclassified drug uridine derivative article cancer cell culture cancer resistance catalysis cell lysate controlled study DNA modification drug synthesis enzyme activity human human cell Catalytic Domain Cell Line, Tumor DNA, Catalytic DNA, Single-Stranded Humans Magnesium Uridine |
| spellingShingle |
10-23 DNAzyme 2′-Deoxy-2′-C-methyl nucleosides 10 23 DNAzyme 2' deoxy 2' c methyluridine deoxyribozyme endonuclease endonuclease RQ1 magnesium ion nucleoside analog unclassified drug uridine derivative article cancer cell culture cancer resistance catalysis cell lysate controlled study DNA modification drug synthesis enzyme activity human human cell Catalytic Domain Cell Line, Tumor DNA, Catalytic DNA, Single-Stranded Humans Magnesium Uridine 10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| topic_facet |
10-23 DNAzyme 2′-Deoxy-2′-C-methyl nucleosides 10 23 DNAzyme 2' deoxy 2' c methyluridine deoxyribozyme endonuclease endonuclease RQ1 magnesium ion nucleoside analog unclassified drug uridine derivative article cancer cell culture cancer resistance catalysis cell lysate controlled study DNA modification drug synthesis enzyme activity human human cell Catalytic Domain Cell Line, Tumor DNA, Catalytic DNA, Single-Stranded Humans Magnesium Uridine |
| description |
The catalytic core of a 10-23 DNAzyme was modified using (2′R), (2′S)-2′-deoxy-2′-C-methyluridine and LNA-T. Catalytic activities under pseudo first order conditions were compared at different Mg 2+ concentrations, indicating that certain 2′-C-methyl modified DNAzymes have significant activities. Resistance against MCF-7 cell lysate and endonuclease RQ1 was also measured, showing that the introduction of 2′-C-methyl-2′-deoxynucleosides increased the stability. © 2010 Elsevier Ltd. All rights reserved. |
| title |
10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| title_short |
10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| title_full |
10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| title_fullStr |
10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| title_full_unstemmed |
10-23 DNAzyme modified with (2′R)- and (2′S)-2′-deoxy- 2′-C-methyluridine in the catalytic core |
| title_sort |
10-23 dnazyme modified with (2′r)- and (2′s)-2′-deoxy- 2′-c-methyluridine in the catalytic core |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0960894X_v20_n15_p4367_Robaldo http://hdl.handle.net/20.500.12110/paper_0960894X_v20_n15_p4367_Robaldo |
| _version_ |
1768543856410755072 |