Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex
Exopolysaccharide (EPS) production by Ganoderma lucidum in response to different culture conditions was studied. Cellulose and glucose, in defined media, resulted in the more efficient enhancers of EPS production among the carbon sources tested. In natural media cultures containing glucose and malt...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09608524_v101_n6_p1941_Papinutti http://hdl.handle.net/20.500.12110/paper_09608524_v101_n6_p1941_Papinutti |
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paper:paper_09608524_v101_n6_p1941_Papinutti2023-06-08T15:57:45Z Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex Papinutti, Víctor Leandro Doehlert factorial design Exopolysaccharide Ganoderma lucidum Water activity Carbon Enzymes Extraction Glucose Metabolites pH effects Culture conditions Exopolysaccharides Factorial design Ganoderma Lucidum Glucose concentration Reducing sugars Water activity Water potential Produced Water carbon cellulose exopolysaccharide glucose macrogol nitrogen glucose macrogol derivative polysaccharide water biological production carbon exopolymer experimental design factor analysis glucose mushroom nutrient use pH plant extract plant water relations polysaccharide article carbon source culture medium fungal biomass fungal strain fungus culture Ganoderma lucidum malt mycelium nonhuman nutrient pH priority journal soil water potential biotechnology chemistry fungus metabolism methodology pH regression analysis time wood Fungi Ganoderma lucidum Biotechnology Carbon Culture Media Fungi Glucose Hydrogen-Ion Concentration Polyethylene Glycols Polysaccharides Regression Analysis Reishi Time Factors Water Wood Exopolysaccharide (EPS) production by Ganoderma lucidum in response to different culture conditions was studied. Cellulose and glucose, in defined media, resulted in the more efficient enhancers of EPS production among the carbon sources tested. In natural media cultures containing glucose and malt extract exhibited a marked increase (up to 29-fold) respect to defined media. Subsequently, high malt extract and glucose concentrations were tested. G. lucidum produced two fractions of EPS, water-soluble and water-insoluble under these culture conditions. The maximum value (15 g L-1) was reached at 21 days in the medium containing 60 g L-1 malt extract and 40 g L-1 glucose. The incomplete utilization of reducing sugars by the fungus in these media suggested that not only did high malt extract and glucose concentrations play a role in EPS production but also the water activity might be involved. A factorial uniform experimental design to test the effect of malt extract, polyethylene glycol (PEG, as water activity depressor), and initial pH on specific EPS production was applied. G. lucidum showed to be a more efficient specific EPS (mg EPS per g mycelium) producer at pH 3.5 in cultures containing the highest PEG and malt extract concentrations. © 2009 Elsevier Ltd. Fil:Papinutti, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09608524_v101_n6_p1941_Papinutti http://hdl.handle.net/20.500.12110/paper_09608524_v101_n6_p1941_Papinutti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Doehlert factorial design Exopolysaccharide Ganoderma lucidum Water activity Carbon Enzymes Extraction Glucose Metabolites pH effects Culture conditions Exopolysaccharides Factorial design Ganoderma Lucidum Glucose concentration Reducing sugars Water activity Water potential Produced Water carbon cellulose exopolysaccharide glucose macrogol nitrogen glucose macrogol derivative polysaccharide water biological production carbon exopolymer experimental design factor analysis glucose mushroom nutrient use pH plant extract plant water relations polysaccharide article carbon source culture medium fungal biomass fungal strain fungus culture Ganoderma lucidum malt mycelium nonhuman nutrient pH priority journal soil water potential biotechnology chemistry fungus metabolism methodology pH regression analysis time wood Fungi Ganoderma lucidum Biotechnology Carbon Culture Media Fungi Glucose Hydrogen-Ion Concentration Polyethylene Glycols Polysaccharides Regression Analysis Reishi Time Factors Water Wood |
| spellingShingle |
Doehlert factorial design Exopolysaccharide Ganoderma lucidum Water activity Carbon Enzymes Extraction Glucose Metabolites pH effects Culture conditions Exopolysaccharides Factorial design Ganoderma Lucidum Glucose concentration Reducing sugars Water activity Water potential Produced Water carbon cellulose exopolysaccharide glucose macrogol nitrogen glucose macrogol derivative polysaccharide water biological production carbon exopolymer experimental design factor analysis glucose mushroom nutrient use pH plant extract plant water relations polysaccharide article carbon source culture medium fungal biomass fungal strain fungus culture Ganoderma lucidum malt mycelium nonhuman nutrient pH priority journal soil water potential biotechnology chemistry fungus metabolism methodology pH regression analysis time wood Fungi Ganoderma lucidum Biotechnology Carbon Culture Media Fungi Glucose Hydrogen-Ion Concentration Polyethylene Glycols Polysaccharides Regression Analysis Reishi Time Factors Water Wood Papinutti, Víctor Leandro Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex |
| topic_facet |
Doehlert factorial design Exopolysaccharide Ganoderma lucidum Water activity Carbon Enzymes Extraction Glucose Metabolites pH effects Culture conditions Exopolysaccharides Factorial design Ganoderma Lucidum Glucose concentration Reducing sugars Water activity Water potential Produced Water carbon cellulose exopolysaccharide glucose macrogol nitrogen glucose macrogol derivative polysaccharide water biological production carbon exopolymer experimental design factor analysis glucose mushroom nutrient use pH plant extract plant water relations polysaccharide article carbon source culture medium fungal biomass fungal strain fungus culture Ganoderma lucidum malt mycelium nonhuman nutrient pH priority journal soil water potential biotechnology chemistry fungus metabolism methodology pH regression analysis time wood Fungi Ganoderma lucidum Biotechnology Carbon Culture Media Fungi Glucose Hydrogen-Ion Concentration Polyethylene Glycols Polysaccharides Regression Analysis Reishi Time Factors Water Wood |
| description |
Exopolysaccharide (EPS) production by Ganoderma lucidum in response to different culture conditions was studied. Cellulose and glucose, in defined media, resulted in the more efficient enhancers of EPS production among the carbon sources tested. In natural media cultures containing glucose and malt extract exhibited a marked increase (up to 29-fold) respect to defined media. Subsequently, high malt extract and glucose concentrations were tested. G. lucidum produced two fractions of EPS, water-soluble and water-insoluble under these culture conditions. The maximum value (15 g L-1) was reached at 21 days in the medium containing 60 g L-1 malt extract and 40 g L-1 glucose. The incomplete utilization of reducing sugars by the fungus in these media suggested that not only did high malt extract and glucose concentrations play a role in EPS production but also the water activity might be involved. A factorial uniform experimental design to test the effect of malt extract, polyethylene glycol (PEG, as water activity depressor), and initial pH on specific EPS production was applied. G. lucidum showed to be a more efficient specific EPS (mg EPS per g mycelium) producer at pH 3.5 in cultures containing the highest PEG and malt extract concentrations. © 2009 Elsevier Ltd. |
| author |
Papinutti, Víctor Leandro |
| author_facet |
Papinutti, Víctor Leandro |
| author_sort |
Papinutti, Víctor Leandro |
| title |
Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex |
| title_short |
Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex |
| title_full |
Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex |
| title_fullStr |
Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex |
| title_full_unstemmed |
Effects of nutrients, pH and water potential on exopolysaccharides production by a fungal strain belonging to Ganoderma lucidum complex |
| title_sort |
effects of nutrients, ph and water potential on exopolysaccharides production by a fungal strain belonging to ganoderma lucidum complex |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09608524_v101_n6_p1941_Papinutti http://hdl.handle.net/20.500.12110/paper_09608524_v101_n6_p1941_Papinutti |
| work_keys_str_mv |
AT papinuttivictorleandro effectsofnutrientsphandwaterpotentialonexopolysaccharidesproductionbyafungalstrainbelongingtoganodermalucidumcomplex |
| _version_ |
1768542044985229312 |