PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis
Two aspects of light are very important for plant development: the length of the light phase or photoperiod and the quality of incoming light. Photoperiod detection allows plants to anticipate the arrival of the next season, whereas light quality, mainly the red to far-red ratio (R:FR), is an early...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09607412_v69_n4_p601_Inigo http://hdl.handle.net/20.500.12110/paper_09607412_v69_n4_p601_Inigo |
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paper:paper_09607412_v69_n4_p601_Inigo2023-06-08T15:57:42Z PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis Alvarez, Mariano Javier Cerdán, Pablo Diego Arabidopsis CONSTANS flowering jasmonate PFT1 photoperiod Arabidopsis CONSTANS flowering Jasmonates PFT1 photoperiod Genes Plants (botany) Transcription apoprotein Arabidopsis protein CONSTANS protein, Arabidopsis cyclopentane derivative DNA binding protein FT protein, Arabidopsis jasmonic acid mediator complex nuclear protein oxylipin PFT1 protein, Arabidopsis PHYB protein, Arabidopsis PHYD protein, Arabidopsis PHYE protein, Arabidopsis phytochrome phytochrome B transcription factor transcriptome animal Arabidopsis article biological model Botrytis butterfly flower Fusarium gene expression regulation genetics light metabolism mutation photoperiodicity physiology plant leaf radiation exposure seedling signal transduction temperature Thysanoptera Animals Apoproteins Arabidopsis Arabidopsis Proteins Botrytis Butterflies Cyclopentanes DNA-Binding Proteins Flowers Fusarium Gene Expression Regulation, Plant Light Mediator Complex Models, Biological Mutation Nuclear Proteins Oxylipins Photoperiod Phytochrome Phytochrome B Plant Leaves Seedling Signal Transduction Temperature Thysanoptera Transcription Factors Transcriptome Arabidopsis Two aspects of light are very important for plant development: the length of the light phase or photoperiod and the quality of incoming light. Photoperiod detection allows plants to anticipate the arrival of the next season, whereas light quality, mainly the red to far-red ratio (R:FR), is an early signal of competition by neighbouring plants. phyB represses flowering by antagonising CO at the transcriptional and post-translational levels. A low R:FR decreases active phyB and consequently increases active CO, which in turn activates the expression of FT, the plant florigen. Other phytochromes like phyD and phyE seem to have redundant roles with phyB. PFT1, the MED25 subunit of the plant Mediator complex, has been proposed to act in the light-quality pathway that regulates flowering time downstream of phyB. However, whether PFT1 signals through CO and its specific mechanism are unclear. Here we show that CO-dependent and-independent mechanisms operate downstream of phyB, phyD and phyE to promote flowering, and that PFT1 is equally able to promote flowering by modulating both CO-dependent and-independent pathways. Our data are consistent with the role of PFT1 as an activator of CO transcription, and also of FT transcription, in a CO-independent manner. Our transcriptome analysis is also consistent with CO and FT genes being the most important flowering targets of PFT1. Furthermore, comparison of the pft1 transcriptome with transcriptomes after fungal and herbivore attack strongly suggests that PFT1 acts as a hub, integrating a variety of interdependent environmental stimuli, including light quality and jasmonic acid-dependent defences. © 2011 Blackwell Publishing Ltd. Fil:Alvarez, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerdán, P.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09607412_v69_n4_p601_Inigo http://hdl.handle.net/20.500.12110/paper_09607412_v69_n4_p601_Inigo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arabidopsis CONSTANS flowering jasmonate PFT1 photoperiod Arabidopsis CONSTANS flowering Jasmonates PFT1 photoperiod Genes Plants (botany) Transcription apoprotein Arabidopsis protein CONSTANS protein, Arabidopsis cyclopentane derivative DNA binding protein FT protein, Arabidopsis jasmonic acid mediator complex nuclear protein oxylipin PFT1 protein, Arabidopsis PHYB protein, Arabidopsis PHYD protein, Arabidopsis PHYE protein, Arabidopsis phytochrome phytochrome B transcription factor transcriptome animal Arabidopsis article biological model Botrytis butterfly flower Fusarium gene expression regulation genetics light metabolism mutation photoperiodicity physiology plant leaf radiation exposure seedling signal transduction temperature Thysanoptera Animals Apoproteins Arabidopsis Arabidopsis Proteins Botrytis Butterflies Cyclopentanes DNA-Binding Proteins Flowers Fusarium Gene Expression Regulation, Plant Light Mediator Complex Models, Biological Mutation Nuclear Proteins Oxylipins Photoperiod Phytochrome Phytochrome B Plant Leaves Seedling Signal Transduction Temperature Thysanoptera Transcription Factors Transcriptome Arabidopsis |
| spellingShingle |
Arabidopsis CONSTANS flowering jasmonate PFT1 photoperiod Arabidopsis CONSTANS flowering Jasmonates PFT1 photoperiod Genes Plants (botany) Transcription apoprotein Arabidopsis protein CONSTANS protein, Arabidopsis cyclopentane derivative DNA binding protein FT protein, Arabidopsis jasmonic acid mediator complex nuclear protein oxylipin PFT1 protein, Arabidopsis PHYB protein, Arabidopsis PHYD protein, Arabidopsis PHYE protein, Arabidopsis phytochrome phytochrome B transcription factor transcriptome animal Arabidopsis article biological model Botrytis butterfly flower Fusarium gene expression regulation genetics light metabolism mutation photoperiodicity physiology plant leaf radiation exposure seedling signal transduction temperature Thysanoptera Animals Apoproteins Arabidopsis Arabidopsis Proteins Botrytis Butterflies Cyclopentanes DNA-Binding Proteins Flowers Fusarium Gene Expression Regulation, Plant Light Mediator Complex Models, Biological Mutation Nuclear Proteins Oxylipins Photoperiod Phytochrome Phytochrome B Plant Leaves Seedling Signal Transduction Temperature Thysanoptera Transcription Factors Transcriptome Arabidopsis Alvarez, Mariano Javier Cerdán, Pablo Diego PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis |
| topic_facet |
Arabidopsis CONSTANS flowering jasmonate PFT1 photoperiod Arabidopsis CONSTANS flowering Jasmonates PFT1 photoperiod Genes Plants (botany) Transcription apoprotein Arabidopsis protein CONSTANS protein, Arabidopsis cyclopentane derivative DNA binding protein FT protein, Arabidopsis jasmonic acid mediator complex nuclear protein oxylipin PFT1 protein, Arabidopsis PHYB protein, Arabidopsis PHYD protein, Arabidopsis PHYE protein, Arabidopsis phytochrome phytochrome B transcription factor transcriptome animal Arabidopsis article biological model Botrytis butterfly flower Fusarium gene expression regulation genetics light metabolism mutation photoperiodicity physiology plant leaf radiation exposure seedling signal transduction temperature Thysanoptera Animals Apoproteins Arabidopsis Arabidopsis Proteins Botrytis Butterflies Cyclopentanes DNA-Binding Proteins Flowers Fusarium Gene Expression Regulation, Plant Light Mediator Complex Models, Biological Mutation Nuclear Proteins Oxylipins Photoperiod Phytochrome Phytochrome B Plant Leaves Seedling Signal Transduction Temperature Thysanoptera Transcription Factors Transcriptome Arabidopsis |
| description |
Two aspects of light are very important for plant development: the length of the light phase or photoperiod and the quality of incoming light. Photoperiod detection allows plants to anticipate the arrival of the next season, whereas light quality, mainly the red to far-red ratio (R:FR), is an early signal of competition by neighbouring plants. phyB represses flowering by antagonising CO at the transcriptional and post-translational levels. A low R:FR decreases active phyB and consequently increases active CO, which in turn activates the expression of FT, the plant florigen. Other phytochromes like phyD and phyE seem to have redundant roles with phyB. PFT1, the MED25 subunit of the plant Mediator complex, has been proposed to act in the light-quality pathway that regulates flowering time downstream of phyB. However, whether PFT1 signals through CO and its specific mechanism are unclear. Here we show that CO-dependent and-independent mechanisms operate downstream of phyB, phyD and phyE to promote flowering, and that PFT1 is equally able to promote flowering by modulating both CO-dependent and-independent pathways. Our data are consistent with the role of PFT1 as an activator of CO transcription, and also of FT transcription, in a CO-independent manner. Our transcriptome analysis is also consistent with CO and FT genes being the most important flowering targets of PFT1. Furthermore, comparison of the pft1 transcriptome with transcriptomes after fungal and herbivore attack strongly suggests that PFT1 acts as a hub, integrating a variety of interdependent environmental stimuli, including light quality and jasmonic acid-dependent defences. © 2011 Blackwell Publishing Ltd. |
| author |
Alvarez, Mariano Javier Cerdán, Pablo Diego |
| author_facet |
Alvarez, Mariano Javier Cerdán, Pablo Diego |
| author_sort |
Alvarez, Mariano Javier |
| title |
PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis |
| title_short |
PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis |
| title_full |
PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis |
| title_fullStr |
PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis |
| title_full_unstemmed |
PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering through CONSTANS dependent and independent mechanisms in Arabidopsis |
| title_sort |
pft1, the med25 subunit of the plant mediator complex, promotes flowering through constans dependent and independent mechanisms in arabidopsis |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09607412_v69_n4_p601_Inigo http://hdl.handle.net/20.500.12110/paper_09607412_v69_n4_p601_Inigo |
| work_keys_str_mv |
AT alvarezmarianojavier pft1themed25subunitoftheplantmediatorcomplexpromotesfloweringthroughconstansdependentandindependentmechanismsinarabidopsis AT cerdanpablodiego pft1themed25subunitoftheplantmediatorcomplexpromotesfloweringthroughconstansdependentandindependentmechanismsinarabidopsis |
| _version_ |
1768546406312706048 |