TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana
In plant sexual reproduction, water and solute movement are tightly regulated, suggesting the involvement of aquaporins. We previously identified TIP5;1 and TIP1;3 as the only Arabidopsis aquaporin genes that are selectively and highly expressed in mature pollen, and showed that they can transport b...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09607412_v64_n6_p1038_Soto http://hdl.handle.net/20.500.12110/paper_09607412_v64_n6_p1038_Soto |
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paper:paper_09607412_v64_n6_p1038_Soto2023-06-08T15:57:42Z TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana Soto, Gabriela Cynthia Fox, Ana Romina Ayub, Nicolás Daniel Mazzella, María Agustina Muschietti, Jorge P. aquaporins mitochondria nitrogen metabolism pH sensing urea Aquaporins Arabidopsis Arabidopsis thaliana Double mutants In-vitro nitrogen metabolism pH sensing Plant sexual reproduction Pollen tube Remobilization Single mutant Solute movement Water transport Xenopus oocyte Amino acids Glycoproteins Metabolism Mitochondria pH sensors Urea Nitrogen aquaporin Arabidopsis protein nitrogen plant RNA TIP5;1 protein, Arabidopsis water Arabidopsis article gene expression regulation genetics metabolism mitochondrion pH phylogeny pollen tube Aquaporins Arabidopsis Arabidopsis Proteins Hydrogen-Ion Concentration Mitochondria Mutagenesis, Insertional Nitrogen Phylogeny Pollen Tube RNA, Plant Water Arabidopsis Arabidopsis thaliana In plant sexual reproduction, water and solute movement are tightly regulated, suggesting the involvement of aquaporins. We previously identified TIP5;1 and TIP1;3 as the only Arabidopsis aquaporin genes that are selectively and highly expressed in mature pollen, and showed that they can transport both water and urea when expressed in Xenopus oocytes. Here, we show that TIP5;1 has unusual characteristics, as its water transport activity is regulated by pH. Analysis of the water transport activity of a mutant version of TIP5;1 (TIP5;1-H131A) and amino acid alignment with other plant aquaporins regulated by pH suggested that a conserved motif is involved in pH sensing. GFP-TIP5;1 is located in the mitochondria of pollen tubes. The single mutants tip1;3 and tip5;1, as well as the tip1;3 tip5;1 double mutant, are fertile, but all mutants had shorter than normal pollen tubes when germinated in vitro in the absence of exogenous nitrogen. Thus, we propose that TIP5;1 and TIP1;3 are involved in nitrogen recycling in pollen tubes of Arabidopsis thaliana. © 2010 The Authors. The Plant Journal © 2010 Blackwell Publishing Ltd. Fil:Soto, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fox, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ayub, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mazzella, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muschietti, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09607412_v64_n6_p1038_Soto http://hdl.handle.net/20.500.12110/paper_09607412_v64_n6_p1038_Soto |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
aquaporins mitochondria nitrogen metabolism pH sensing urea Aquaporins Arabidopsis Arabidopsis thaliana Double mutants In-vitro nitrogen metabolism pH sensing Plant sexual reproduction Pollen tube Remobilization Single mutant Solute movement Water transport Xenopus oocyte Amino acids Glycoproteins Metabolism Mitochondria pH sensors Urea Nitrogen aquaporin Arabidopsis protein nitrogen plant RNA TIP5;1 protein, Arabidopsis water Arabidopsis article gene expression regulation genetics metabolism mitochondrion pH phylogeny pollen tube Aquaporins Arabidopsis Arabidopsis Proteins Hydrogen-Ion Concentration Mitochondria Mutagenesis, Insertional Nitrogen Phylogeny Pollen Tube RNA, Plant Water Arabidopsis Arabidopsis thaliana |
| spellingShingle |
aquaporins mitochondria nitrogen metabolism pH sensing urea Aquaporins Arabidopsis Arabidopsis thaliana Double mutants In-vitro nitrogen metabolism pH sensing Plant sexual reproduction Pollen tube Remobilization Single mutant Solute movement Water transport Xenopus oocyte Amino acids Glycoproteins Metabolism Mitochondria pH sensors Urea Nitrogen aquaporin Arabidopsis protein nitrogen plant RNA TIP5;1 protein, Arabidopsis water Arabidopsis article gene expression regulation genetics metabolism mitochondrion pH phylogeny pollen tube Aquaporins Arabidopsis Arabidopsis Proteins Hydrogen-Ion Concentration Mitochondria Mutagenesis, Insertional Nitrogen Phylogeny Pollen Tube RNA, Plant Water Arabidopsis Arabidopsis thaliana Soto, Gabriela Cynthia Fox, Ana Romina Ayub, Nicolás Daniel Mazzella, María Agustina Muschietti, Jorge P. TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana |
| topic_facet |
aquaporins mitochondria nitrogen metabolism pH sensing urea Aquaporins Arabidopsis Arabidopsis thaliana Double mutants In-vitro nitrogen metabolism pH sensing Plant sexual reproduction Pollen tube Remobilization Single mutant Solute movement Water transport Xenopus oocyte Amino acids Glycoproteins Metabolism Mitochondria pH sensors Urea Nitrogen aquaporin Arabidopsis protein nitrogen plant RNA TIP5;1 protein, Arabidopsis water Arabidopsis article gene expression regulation genetics metabolism mitochondrion pH phylogeny pollen tube Aquaporins Arabidopsis Arabidopsis Proteins Hydrogen-Ion Concentration Mitochondria Mutagenesis, Insertional Nitrogen Phylogeny Pollen Tube RNA, Plant Water Arabidopsis Arabidopsis thaliana |
| description |
In plant sexual reproduction, water and solute movement are tightly regulated, suggesting the involvement of aquaporins. We previously identified TIP5;1 and TIP1;3 as the only Arabidopsis aquaporin genes that are selectively and highly expressed in mature pollen, and showed that they can transport both water and urea when expressed in Xenopus oocytes. Here, we show that TIP5;1 has unusual characteristics, as its water transport activity is regulated by pH. Analysis of the water transport activity of a mutant version of TIP5;1 (TIP5;1-H131A) and amino acid alignment with other plant aquaporins regulated by pH suggested that a conserved motif is involved in pH sensing. GFP-TIP5;1 is located in the mitochondria of pollen tubes. The single mutants tip1;3 and tip5;1, as well as the tip1;3 tip5;1 double mutant, are fertile, but all mutants had shorter than normal pollen tubes when germinated in vitro in the absence of exogenous nitrogen. Thus, we propose that TIP5;1 and TIP1;3 are involved in nitrogen recycling in pollen tubes of Arabidopsis thaliana. © 2010 The Authors. The Plant Journal © 2010 Blackwell Publishing Ltd. |
| author |
Soto, Gabriela Cynthia Fox, Ana Romina Ayub, Nicolás Daniel Mazzella, María Agustina Muschietti, Jorge P. |
| author_facet |
Soto, Gabriela Cynthia Fox, Ana Romina Ayub, Nicolás Daniel Mazzella, María Agustina Muschietti, Jorge P. |
| author_sort |
Soto, Gabriela Cynthia |
| title |
TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana |
| title_short |
TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana |
| title_full |
TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana |
| title_fullStr |
TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana |
| title_full_unstemmed |
TIP5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in Arabidopsis thaliana |
| title_sort |
tip5;1 is an aquaporin specifically targeted to pollen mitochondria and is probably involved in nitrogen remobilization in arabidopsis thaliana |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09607412_v64_n6_p1038_Soto http://hdl.handle.net/20.500.12110/paper_09607412_v64_n6_p1038_Soto |
| work_keys_str_mv |
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| _version_ |
1768545984981237760 |