In vivo regulation of the steroidogenic activity of rat luteal cells by insulin

The aim of the present study was to determine the long-term effects of insulin treatment on luteal cell function. For this purpose, superovulated prepubertal rats were treated with insulin (group I) or vehicle (group C) for 9 days. Serum progesterone (P4) levels were increased in the insulin-treated...

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Autores principales:	Stein, Paula, Tesone, Marta
Publicado:	1995
Materias:	20alpha dihydroprogesterone cytochrome p450 insulin luteinizing hormone receptor progesterone animal cell animal experiment article controlled study female immunoblotting luteal cell nonhuman rat steroidogenesis subcutaneous drug administration Animal Cholesterol Side-Chain Cleavage Enzyme Corpus Luteum Dihydroprogesterone Dose-Response Relationship, Drug Drug Interactions Female Insulin Luteinizing Hormone Ovulation Progesterone Puberty Rats Rats, Sprague-Dawley Receptors, LH Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v52_n4_p329_Stein http://hdl.handle.net/20.500.12110/paper_09600760_v52_n4_p329_Stein
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09600760_v52_n4_p329_Stein
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spelling	paper:paper_09600760_v52_n4_p329_Stein2023-06-08T15:57:24Z In vivo regulation of the steroidogenic activity of rat luteal cells by insulin Stein, Paula Tesone, Marta 20alpha dihydroprogesterone cytochrome p450 insulin luteinizing hormone receptor progesterone animal cell animal experiment article controlled study female immunoblotting luteal cell nonhuman rat steroidogenesis subcutaneous drug administration Animal Cholesterol Side-Chain Cleavage Enzyme Corpus Luteum Dihydroprogesterone Dose-Response Relationship, Drug Drug Interactions Female Insulin Luteinizing Hormone Ovulation Progesterone Puberty Rats Rats, Sprague-Dawley Receptors, LH Support, Non-U.S. Gov't The aim of the present study was to determine the long-term effects of insulin treatment on luteal cell function. For this purpose, superovulated prepubertal rats were treated with insulin (group I) or vehicle (group C) for 9 days. Serum progesterone (P4) levels were increased in the insulin-treated group (55 ± 10 vs 134 ± 31 ng/ml, P < 0.05). Isolated luteal cells were incubated 3 h, and P4 and 20α-hydroxy-progesterone (20α-OH-P) were measured in the incubation media. A decrease in P4 levels and an increase in 20α-OH-P values [P4 (ng/ml): C = 26.6 ± 0.3; I = 20 ± 2; 20α-OH-P (ng/ml): C = 62 ± 2; I: 120 ± 7; P < 0.01] were observed in group I. In addition, progestagen (P4 + 20α-OH-P) levels were higher in group I (C = 88 ± 2; I = 140 ± 9 ng/ml; P < 0.001). When cytochrome P450SCC contents were measured by immunoblotting, a marked increase was observed in luteal cells obtained from group I. LH receptor numbers were decreased in luteal cells isolated from group I (C = 388,834 ± 14,146; I = 303,057 ± 13,392 sites/cell; P < 0.001) with a concomitantly diminished LH responsiveness. It is concluded that in vivo treatment of superovulated rats with insulin increases luteal progestagen production by increasing the content of cytochrome P450SCC. © 1995. Fil:Stein, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v52_n4_p329_Stein http://hdl.handle.net/20.500.12110/paper_09600760_v52_n4_p329_Stein
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	20alpha dihydroprogesterone cytochrome p450 insulin luteinizing hormone receptor progesterone animal cell animal experiment article controlled study female immunoblotting luteal cell nonhuman rat steroidogenesis subcutaneous drug administration Animal Cholesterol Side-Chain Cleavage Enzyme Corpus Luteum Dihydroprogesterone Dose-Response Relationship, Drug Drug Interactions Female Insulin Luteinizing Hormone Ovulation Progesterone Puberty Rats Rats, Sprague-Dawley Receptors, LH Support, Non-U.S. Gov't
spellingShingle	20alpha dihydroprogesterone cytochrome p450 insulin luteinizing hormone receptor progesterone animal cell animal experiment article controlled study female immunoblotting luteal cell nonhuman rat steroidogenesis subcutaneous drug administration Animal Cholesterol Side-Chain Cleavage Enzyme Corpus Luteum Dihydroprogesterone Dose-Response Relationship, Drug Drug Interactions Female Insulin Luteinizing Hormone Ovulation Progesterone Puberty Rats Rats, Sprague-Dawley Receptors, LH Support, Non-U.S. Gov't Stein, Paula Tesone, Marta In vivo regulation of the steroidogenic activity of rat luteal cells by insulin
topic_facet	20alpha dihydroprogesterone cytochrome p450 insulin luteinizing hormone receptor progesterone animal cell animal experiment article controlled study female immunoblotting luteal cell nonhuman rat steroidogenesis subcutaneous drug administration Animal Cholesterol Side-Chain Cleavage Enzyme Corpus Luteum Dihydroprogesterone Dose-Response Relationship, Drug Drug Interactions Female Insulin Luteinizing Hormone Ovulation Progesterone Puberty Rats Rats, Sprague-Dawley Receptors, LH Support, Non-U.S. Gov't
description	The aim of the present study was to determine the long-term effects of insulin treatment on luteal cell function. For this purpose, superovulated prepubertal rats were treated with insulin (group I) or vehicle (group C) for 9 days. Serum progesterone (P4) levels were increased in the insulin-treated group (55 ± 10 vs 134 ± 31 ng/ml, P < 0.05). Isolated luteal cells were incubated 3 h, and P4 and 20α-hydroxy-progesterone (20α-OH-P) were measured in the incubation media. A decrease in P4 levels and an increase in 20α-OH-P values [P4 (ng/ml): C = 26.6 ± 0.3; I = 20 ± 2; 20α-OH-P (ng/ml): C = 62 ± 2; I: 120 ± 7; P < 0.01] were observed in group I. In addition, progestagen (P4 + 20α-OH-P) levels were higher in group I (C = 88 ± 2; I = 140 ± 9 ng/ml; P < 0.001). When cytochrome P450SCC contents were measured by immunoblotting, a marked increase was observed in luteal cells obtained from group I. LH receptor numbers were decreased in luteal cells isolated from group I (C = 388,834 ± 14,146; I = 303,057 ± 13,392 sites/cell; P < 0.001) with a concomitantly diminished LH responsiveness. It is concluded that in vivo treatment of superovulated rats with insulin increases luteal progestagen production by increasing the content of cytochrome P450SCC. © 1995.
author	Stein, Paula Tesone, Marta
author_facet	Stein, Paula Tesone, Marta
author_sort	Stein, Paula
title	In vivo regulation of the steroidogenic activity of rat luteal cells by insulin
title_short	In vivo regulation of the steroidogenic activity of rat luteal cells by insulin
title_full	In vivo regulation of the steroidogenic activity of rat luteal cells by insulin
title_fullStr	In vivo regulation of the steroidogenic activity of rat luteal cells by insulin
title_full_unstemmed	In vivo regulation of the steroidogenic activity of rat luteal cells by insulin
title_sort	in vivo regulation of the steroidogenic activity of rat luteal cells by insulin
publishDate	1995
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v52_n4_p329_Stein http://hdl.handle.net/20.500.12110/paper_09600760_v52_n4_p329_Stein
work_keys_str_mv	AT steinpaula invivoregulationofthesteroidogenicactivityofratlutealcellsbyinsulin AT tesonemarta invivoregulationofthesteroidogenicactivityofratlutealcellsbyinsulin
_version_	1768545840630071296

In vivo regulation of the steroidogenic activity of rat luteal cells by insulin

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