Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells
We had previously reported that juvenile hormone III (JH III) and the JH analogue 2-(4-phenoxy phenoxy)-ethoxytetrahydropyran exert inhibitory effects on progesterone synthesis by blocking cAMP production in hCG-stimulated MA-10 Leydig tumor cells. In the present study, the effects of JH analogue up...
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1995
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v52_n1_p83_Vladusic http://hdl.handle.net/20.500.12110/paper_09600760_v52_n1_p83_Vladusic |
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paper:paper_09600760_v52_n1_p83_Vladusic2025-07-30T18:33:31Z Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells 2 (4 phenoxyphenoxy)ethoxytetrahydropyran 20 hydroxycholesterol 25 hydroxycholesterol 3 decyldimethylsilyl n [2 (4 methylphenyl) 1 phenylethyl]propionamide 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide bucladesine cholesterol esterase cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin enzyme inhibitor juvenile hormone derivative pregnenolone unclassified drug animal cell article cholesterol transport controlled study enzyme activity leydig cell tumor male nonhuman progesterone synthesis steroidogenesis Bucladesine Cell Membrane Cholesterol Cholesterol Esterase Cholesterol Esters Hydroxycholesterols Juvenile Hormones Leydig Cell Tumor Progesterone Pyrans Sesquiterpenes Support, Non-U.S. Gov't We had previously reported that juvenile hormone III (JH III) and the JH analogue 2-(4-phenoxy phenoxy)-ethoxytetrahydropyran exert inhibitory effects on progesterone synthesis by blocking cAMP production in hCG-stimulated MA-10 Leydig tumor cells. In the present study, the effects of JH analogue upon the biosynthetic pathway of progesterone synthesis have been examined. Our results demonstrated that JH analogue inhibited progesterone production even in the presence of 20-hydroxycholesterol or 25-hydroxycholesterol. Furthermore, although JH analogue inhibited pregnenolone production in hCG-stimulated MA-10 cells the activity of the 3β-hydroxysteroid dehydrogenase (3β-HSD) was unaffected. These data suggest that JH analogue might inhibit the steroidogenic pathway in Leydig tumor cells by inhibiting the activity of the cholesterol side chain cleavage (CSCC) enzymatic complex. The JH analogue was also evaluated for inhibitory actions on cholesterol availability. An important effect of this compound was the interference with the cellular process of plasma membrane cholesterol internalization. Moreover, JH analogue inhibited not only the use of cholesterol ester for steroid biosynthesis under Bt2cAMP stimulation, but also the cholesterol ester hydrolase (CEH) activity in MA-10 Leydig tumor cells. © 1995. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v52_n1_p83_Vladusic http://hdl.handle.net/20.500.12110/paper_09600760_v52_n1_p83_Vladusic |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
2 (4 phenoxyphenoxy)ethoxytetrahydropyran 20 hydroxycholesterol 25 hydroxycholesterol 3 decyldimethylsilyl n [2 (4 methylphenyl) 1 phenylethyl]propionamide 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide bucladesine cholesterol esterase cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin enzyme inhibitor juvenile hormone derivative pregnenolone unclassified drug animal cell article cholesterol transport controlled study enzyme activity leydig cell tumor male nonhuman progesterone synthesis steroidogenesis Bucladesine Cell Membrane Cholesterol Cholesterol Esterase Cholesterol Esters Hydroxycholesterols Juvenile Hormones Leydig Cell Tumor Progesterone Pyrans Sesquiterpenes Support, Non-U.S. Gov't |
| spellingShingle |
2 (4 phenoxyphenoxy)ethoxytetrahydropyran 20 hydroxycholesterol 25 hydroxycholesterol 3 decyldimethylsilyl n [2 (4 methylphenyl) 1 phenylethyl]propionamide 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide bucladesine cholesterol esterase cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin enzyme inhibitor juvenile hormone derivative pregnenolone unclassified drug animal cell article cholesterol transport controlled study enzyme activity leydig cell tumor male nonhuman progesterone synthesis steroidogenesis Bucladesine Cell Membrane Cholesterol Cholesterol Esterase Cholesterol Esters Hydroxycholesterols Juvenile Hormones Leydig Cell Tumor Progesterone Pyrans Sesquiterpenes Support, Non-U.S. Gov't Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells |
| topic_facet |
2 (4 phenoxyphenoxy)ethoxytetrahydropyran 20 hydroxycholesterol 25 hydroxycholesterol 3 decyldimethylsilyl n [2 (4 methylphenyl) 1 phenylethyl]propionamide 3(or 17)beta hydroxysteroid dehydrogenase aminoglutethimide bucladesine cholesterol esterase cholesterol monooxygenase (side chain cleaving) chorionic gonadotropin enzyme inhibitor juvenile hormone derivative pregnenolone unclassified drug animal cell article cholesterol transport controlled study enzyme activity leydig cell tumor male nonhuman progesterone synthesis steroidogenesis Bucladesine Cell Membrane Cholesterol Cholesterol Esterase Cholesterol Esters Hydroxycholesterols Juvenile Hormones Leydig Cell Tumor Progesterone Pyrans Sesquiterpenes Support, Non-U.S. Gov't |
| description |
We had previously reported that juvenile hormone III (JH III) and the JH analogue 2-(4-phenoxy phenoxy)-ethoxytetrahydropyran exert inhibitory effects on progesterone synthesis by blocking cAMP production in hCG-stimulated MA-10 Leydig tumor cells. In the present study, the effects of JH analogue upon the biosynthetic pathway of progesterone synthesis have been examined. Our results demonstrated that JH analogue inhibited progesterone production even in the presence of 20-hydroxycholesterol or 25-hydroxycholesterol. Furthermore, although JH analogue inhibited pregnenolone production in hCG-stimulated MA-10 cells the activity of the 3β-hydroxysteroid dehydrogenase (3β-HSD) was unaffected. These data suggest that JH analogue might inhibit the steroidogenic pathway in Leydig tumor cells by inhibiting the activity of the cholesterol side chain cleavage (CSCC) enzymatic complex. The JH analogue was also evaluated for inhibitory actions on cholesterol availability. An important effect of this compound was the interference with the cellular process of plasma membrane cholesterol internalization. Moreover, JH analogue inhibited not only the use of cholesterol ester for steroid biosynthesis under Bt2cAMP stimulation, but also the cholesterol ester hydrolase (CEH) activity in MA-10 Leydig tumor cells. © 1995. |
| title |
Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells |
| title_short |
Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells |
| title_full |
Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells |
| title_fullStr |
Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells |
| title_full_unstemmed |
Regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in MA-10 Leydig tumor cells |
| title_sort |
regulation of the cholesterol ester cycle and progesterone synthesis by juvenile hormone in ma-10 leydig tumor cells |
| publishDate |
1995 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v52_n1_p83_Vladusic http://hdl.handle.net/20.500.12110/paper_09600760_v52_n1_p83_Vladusic |
| _version_ |
1840323037442867200 |