Effects of juvenile hormone on mammalian steroidogenesis

The effects of juvenile hormone-III (JH-III) and the JH analogue 2-(4-phenoxyphenoxy)-ethoxytetrahydropiran on testicular steroidogenesis were studied. By using cultured MA-10 Leydig tumor cells as a model, these compounds were found to be potent inhibitors of LH/hCG steroidogenic action in a dose-d...

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Autores principales:	Vladusic, Esteban Arturo, Stoka, Ángel de la Merced, Rodríguez, Juan Bautista, Gros, Eduardo Gervasio, Charreau, Eduardo Hernán
Publicado:	1994
Materias:	adenylate cyclase cyclic amp juvenile hormone juvenile hormone derivative article cell culture leydig cell tumor nonhuman progesterone synthesis steroidogenesis testis Animal Cell Line Cholera Toxin Chorionic Gonadotropin Cyclic AMP Guanosine Triphosphate Juvenile Hormones Leydig Cells Male Mammals Progesterone Signal Transduction Support, Non-U.S. Gov't Mammalia
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v50_n3-4_p181_Vladusic http://hdl.handle.net/20.500.12110/paper_09600760_v50_n3-4_p181_Vladusic
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09600760_v50_n3-4_p181_Vladusic
record_format	dspace
spelling	paper:paper_09600760_v50_n3-4_p181_Vladusic2023-06-08T15:57:23Z Effects of juvenile hormone on mammalian steroidogenesis Vladusic, Esteban Arturo Stoka, Ángel de la Merced Rodríguez, Juan Bautista Gros, Eduardo Gervasio Charreau, Eduardo Hernán adenylate cyclase cyclic amp juvenile hormone juvenile hormone derivative article cell culture leydig cell tumor nonhuman progesterone synthesis steroidogenesis testis Animal Cell Line Cholera Toxin Chorionic Gonadotropin Cyclic AMP Guanosine Triphosphate Juvenile Hormones Leydig Cells Male Mammals Progesterone Signal Transduction Support, Non-U.S. Gov't Mammalia The effects of juvenile hormone-III (JH-III) and the JH analogue 2-(4-phenoxyphenoxy)-ethoxytetrahydropiran on testicular steroidogenesis were studied. By using cultured MA-10 Leydig tumor cells as a model, these compounds were found to be potent inhibitors of LH/hCG steroidogenic action in a dose-dependent manner. Scatchard plot analysis of the binding data indicated that the JH analogue did not significantly alter the affinity nor the number of hCG binding sites, as well as GTP binding to plasma membranes. JH analogue inhibited the stimulatory action of both cholera toxin and forskolin on cAMP production and the concomitant steroidogenic response. JH analogue inhibited (Bu) 2 cAMP-stimulated progesterone synthesis, indicating that a process downstream to the adenylyl cyclase in the steroidogenic pathway is also affected. © 1994. Fil:Vladusic, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Stoka, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodriguez, J.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gros, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v50_n3-4_p181_Vladusic http://hdl.handle.net/20.500.12110/paper_09600760_v50_n3-4_p181_Vladusic
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	adenylate cyclase cyclic amp juvenile hormone juvenile hormone derivative article cell culture leydig cell tumor nonhuman progesterone synthesis steroidogenesis testis Animal Cell Line Cholera Toxin Chorionic Gonadotropin Cyclic AMP Guanosine Triphosphate Juvenile Hormones Leydig Cells Male Mammals Progesterone Signal Transduction Support, Non-U.S. Gov't Mammalia
spellingShingle	adenylate cyclase cyclic amp juvenile hormone juvenile hormone derivative article cell culture leydig cell tumor nonhuman progesterone synthesis steroidogenesis testis Animal Cell Line Cholera Toxin Chorionic Gonadotropin Cyclic AMP Guanosine Triphosphate Juvenile Hormones Leydig Cells Male Mammals Progesterone Signal Transduction Support, Non-U.S. Gov't Mammalia Vladusic, Esteban Arturo Stoka, Ángel de la Merced Rodríguez, Juan Bautista Gros, Eduardo Gervasio Charreau, Eduardo Hernán Effects of juvenile hormone on mammalian steroidogenesis
topic_facet	adenylate cyclase cyclic amp juvenile hormone juvenile hormone derivative article cell culture leydig cell tumor nonhuman progesterone synthesis steroidogenesis testis Animal Cell Line Cholera Toxin Chorionic Gonadotropin Cyclic AMP Guanosine Triphosphate Juvenile Hormones Leydig Cells Male Mammals Progesterone Signal Transduction Support, Non-U.S. Gov't Mammalia
description	The effects of juvenile hormone-III (JH-III) and the JH analogue 2-(4-phenoxyphenoxy)-ethoxytetrahydropiran on testicular steroidogenesis were studied. By using cultured MA-10 Leydig tumor cells as a model, these compounds were found to be potent inhibitors of LH/hCG steroidogenic action in a dose-dependent manner. Scatchard plot analysis of the binding data indicated that the JH analogue did not significantly alter the affinity nor the number of hCG binding sites, as well as GTP binding to plasma membranes. JH analogue inhibited the stimulatory action of both cholera toxin and forskolin on cAMP production and the concomitant steroidogenic response. JH analogue inhibited (Bu) 2 cAMP-stimulated progesterone synthesis, indicating that a process downstream to the adenylyl cyclase in the steroidogenic pathway is also affected. © 1994.
author	Vladusic, Esteban Arturo Stoka, Ángel de la Merced Rodríguez, Juan Bautista Gros, Eduardo Gervasio Charreau, Eduardo Hernán
author_facet	Vladusic, Esteban Arturo Stoka, Ángel de la Merced Rodríguez, Juan Bautista Gros, Eduardo Gervasio Charreau, Eduardo Hernán
author_sort	Vladusic, Esteban Arturo
title	Effects of juvenile hormone on mammalian steroidogenesis
title_short	Effects of juvenile hormone on mammalian steroidogenesis
title_full	Effects of juvenile hormone on mammalian steroidogenesis
title_fullStr	Effects of juvenile hormone on mammalian steroidogenesis
title_full_unstemmed	Effects of juvenile hormone on mammalian steroidogenesis
title_sort	effects of juvenile hormone on mammalian steroidogenesis
publishDate	1994
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v50_n3-4_p181_Vladusic http://hdl.handle.net/20.500.12110/paper_09600760_v50_n3-4_p181_Vladusic
work_keys_str_mv	AT vladusicestebanarturo effectsofjuvenilehormoneonmammaliansteroidogenesis AT stokaangeldelamerced effectsofjuvenilehormoneonmammaliansteroidogenesis AT rodriguezjuanbautista effectsofjuvenilehormoneonmammaliansteroidogenesis AT groseduardogervasio effectsofjuvenilehormoneonmammaliansteroidogenesis AT charreaueduardohernan effectsofjuvenilehormoneonmammaliansteroidogenesis
_version_	1768545565737484288

Effects of juvenile hormone on mammalian steroidogenesis

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