Dehydroepiandrosterone and metformin modulate progesterone-induced blocking factor (PIBF), cyclooxygenase 2 (COX2) and cytokines in early pregnant mice
The present study examined the mechanism by which metformin (N,N′-dimethylbiguanide) prevents embryonic resorption induced in mice by dehydroepiandrosterone (DHEA). Treatment with DHEA (60 mg/kg, s.c. 24 and 48 h post-implantation) induces embryo resorption of early pregnant BALB/c mice while simult...
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2008
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v111_n3-5_p200_Luchetti http://hdl.handle.net/20.500.12110/paper_09600760_v111_n3-5_p200_Luchetti |
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paper:paper_09600760_v111_n3-5_p200_Luchetti2023-06-08T15:57:19Z Dehydroepiandrosterone and metformin modulate progesterone-induced blocking factor (PIBF), cyclooxygenase 2 (COX2) and cytokines in early pregnant mice Luchetti, Carolina Griselda Paz, Dante Agustin Motta, Alicia Beatriz Cytokines Dehydroepiandrosterone Metformin PIBF Pregnancy Progesterone cyclooxygenase 2 cytokine gamma interferon interleukin 2 interleukin 4 interleukin 6 metformin placenta protein prasterone progesterone progesterone induced blocking factor protein prostaglandin animal experiment animal model animal tissue article blocking factor controlled study cytokine production drug mechanism embryo resorption enzyme linked immunosorbent assay female immunohistochemistry mouse nonhuman protein blood level protein expression Animals Cyclooxygenase 2 Cytokines Dehydroepiandrosterone Embryo Implantation Embryo Loss Embryo, Mammalian Female Hypoglycemic Agents Male Metformin Mice Mice, Inbred BALB C Pregnancy Pregnancy Proteins Pregnancy, Animal Suppressor Factors, Immunologic Animalia Mus The present study examined the mechanism by which metformin (N,N′-dimethylbiguanide) prevents embryonic resorption induced in mice by dehydroepiandrosterone (DHEA). Treatment with DHEA (60 mg/kg, s.c. 24 and 48 h post-implantation) induces embryo resorption of early pregnant BALB/c mice while simultaneous treatment with metformin (240 mg/kg, oral 24 and 48 h post-implantation) prevents it. During pregnancy progesterone-induced blocking factor (PIBF) modulates prostaglandins (PGs) and cytokine production. These findings prompted us to investigate the effect of DHEA and metformin on both PIBF and cyclooxygenase 2 (COX2) expressions at the implantation sites, as well as cytokine production. PIBF and COX2 expression were detected by immunohistochemistry from DHEA and DHEA+ metformin treated 8 days-pregnant mice and serum cytokine levels of these animals were determined by ELISA. DHEA treatment both abolished PIBF expression and increased COX2 expression. Embryo resorption correlates with the lack of PIBF expression, diminished IL-6 levels and increased IL-2 concentration while metformin was able to reverse the effect of DHEA on both PIBF and COX2 expression and IL-6 levels. We concluded that hyperandrogenization induces embryo resorption in early pregnancy diminishing PIBF in implantation sites, having a pro-inflammatory effect. Metformin is able to prevent such effects. © 2008 Elsevier Ltd. All rights reserved. Fil:Luchetti, C.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Motta, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v111_n3-5_p200_Luchetti http://hdl.handle.net/20.500.12110/paper_09600760_v111_n3-5_p200_Luchetti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cytokines Dehydroepiandrosterone Metformin PIBF Pregnancy Progesterone cyclooxygenase 2 cytokine gamma interferon interleukin 2 interleukin 4 interleukin 6 metformin placenta protein prasterone progesterone progesterone induced blocking factor protein prostaglandin animal experiment animal model animal tissue article blocking factor controlled study cytokine production drug mechanism embryo resorption enzyme linked immunosorbent assay female immunohistochemistry mouse nonhuman protein blood level protein expression Animals Cyclooxygenase 2 Cytokines Dehydroepiandrosterone Embryo Implantation Embryo Loss Embryo, Mammalian Female Hypoglycemic Agents Male Metformin Mice Mice, Inbred BALB C Pregnancy Pregnancy Proteins Pregnancy, Animal Suppressor Factors, Immunologic Animalia Mus |
| spellingShingle |
Cytokines Dehydroepiandrosterone Metformin PIBF Pregnancy Progesterone cyclooxygenase 2 cytokine gamma interferon interleukin 2 interleukin 4 interleukin 6 metformin placenta protein prasterone progesterone progesterone induced blocking factor protein prostaglandin animal experiment animal model animal tissue article blocking factor controlled study cytokine production drug mechanism embryo resorption enzyme linked immunosorbent assay female immunohistochemistry mouse nonhuman protein blood level protein expression Animals Cyclooxygenase 2 Cytokines Dehydroepiandrosterone Embryo Implantation Embryo Loss Embryo, Mammalian Female Hypoglycemic Agents Male Metformin Mice Mice, Inbred BALB C Pregnancy Pregnancy Proteins Pregnancy, Animal Suppressor Factors, Immunologic Animalia Mus Luchetti, Carolina Griselda Paz, Dante Agustin Motta, Alicia Beatriz Dehydroepiandrosterone and metformin modulate progesterone-induced blocking factor (PIBF), cyclooxygenase 2 (COX2) and cytokines in early pregnant mice |
| topic_facet |
Cytokines Dehydroepiandrosterone Metformin PIBF Pregnancy Progesterone cyclooxygenase 2 cytokine gamma interferon interleukin 2 interleukin 4 interleukin 6 metformin placenta protein prasterone progesterone progesterone induced blocking factor protein prostaglandin animal experiment animal model animal tissue article blocking factor controlled study cytokine production drug mechanism embryo resorption enzyme linked immunosorbent assay female immunohistochemistry mouse nonhuman protein blood level protein expression Animals Cyclooxygenase 2 Cytokines Dehydroepiandrosterone Embryo Implantation Embryo Loss Embryo, Mammalian Female Hypoglycemic Agents Male Metformin Mice Mice, Inbred BALB C Pregnancy Pregnancy Proteins Pregnancy, Animal Suppressor Factors, Immunologic Animalia Mus |
| description |
The present study examined the mechanism by which metformin (N,N′-dimethylbiguanide) prevents embryonic resorption induced in mice by dehydroepiandrosterone (DHEA). Treatment with DHEA (60 mg/kg, s.c. 24 and 48 h post-implantation) induces embryo resorption of early pregnant BALB/c mice while simultaneous treatment with metformin (240 mg/kg, oral 24 and 48 h post-implantation) prevents it. During pregnancy progesterone-induced blocking factor (PIBF) modulates prostaglandins (PGs) and cytokine production. These findings prompted us to investigate the effect of DHEA and metformin on both PIBF and cyclooxygenase 2 (COX2) expressions at the implantation sites, as well as cytokine production. PIBF and COX2 expression were detected by immunohistochemistry from DHEA and DHEA+ metformin treated 8 days-pregnant mice and serum cytokine levels of these animals were determined by ELISA. DHEA treatment both abolished PIBF expression and increased COX2 expression. Embryo resorption correlates with the lack of PIBF expression, diminished IL-6 levels and increased IL-2 concentration while metformin was able to reverse the effect of DHEA on both PIBF and COX2 expression and IL-6 levels. We concluded that hyperandrogenization induces embryo resorption in early pregnancy diminishing PIBF in implantation sites, having a pro-inflammatory effect. Metformin is able to prevent such effects. © 2008 Elsevier Ltd. All rights reserved. |
| author |
Luchetti, Carolina Griselda Paz, Dante Agustin Motta, Alicia Beatriz |
| author_facet |
Luchetti, Carolina Griselda Paz, Dante Agustin Motta, Alicia Beatriz |
| author_sort |
Luchetti, Carolina Griselda |
| title |
Dehydroepiandrosterone and metformin modulate progesterone-induced blocking factor (PIBF), cyclooxygenase 2 (COX2) and cytokines in early pregnant mice |
| title_short |
Dehydroepiandrosterone and metformin modulate progesterone-induced blocking factor (PIBF), cyclooxygenase 2 (COX2) and cytokines in early pregnant mice |
| title_full |
Dehydroepiandrosterone and metformin modulate progesterone-induced blocking factor (PIBF), cyclooxygenase 2 (COX2) and cytokines in early pregnant mice |
| title_fullStr |
Dehydroepiandrosterone and metformin modulate progesterone-induced blocking factor (PIBF), cyclooxygenase 2 (COX2) and cytokines in early pregnant mice |
| title_full_unstemmed |
Dehydroepiandrosterone and metformin modulate progesterone-induced blocking factor (PIBF), cyclooxygenase 2 (COX2) and cytokines in early pregnant mice |
| title_sort |
dehydroepiandrosterone and metformin modulate progesterone-induced blocking factor (pibf), cyclooxygenase 2 (cox2) and cytokines in early pregnant mice |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09600760_v111_n3-5_p200_Luchetti http://hdl.handle.net/20.500.12110/paper_09600760_v111_n3-5_p200_Luchetti |
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| _version_ |
1768543809325498368 |