The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids
The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-h exadecylglycero...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09596658_v7_n6_p731_Agusti http://hdl.handle.net/20.500.12110/paper_09596658_v7_n6_p731_Agusti |
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paper:paper_09596658_v7_n6_p731_Agusti2025-07-30T18:33:15Z The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids GPI Trans-sialidase Trypanosoma cruzi lipid palmitic acid sialidase article immunoprecipitation nonhuman priority journal thin layer chromatography trypanosoma cruzi Animals Chromatography, Thin Layer Glycosylphosphatidylinositols Membrane Lipids Mice Neuraminidase Phosphatidylinositol Diacylglycerol-Lyase Phospholipase C Substrate Specificity Trypanosoma cruzi Trypanosoma Trypanosoma cruzi The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-h exadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T. ruzi was made. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09596658_v7_n6_p731_Agusti http://hdl.handle.net/20.500.12110/paper_09596658_v7_n6_p731_Agusti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
GPI Trans-sialidase Trypanosoma cruzi lipid palmitic acid sialidase article immunoprecipitation nonhuman priority journal thin layer chromatography trypanosoma cruzi Animals Chromatography, Thin Layer Glycosylphosphatidylinositols Membrane Lipids Mice Neuraminidase Phosphatidylinositol Diacylglycerol-Lyase Phospholipase C Substrate Specificity Trypanosoma cruzi Trypanosoma Trypanosoma cruzi |
| spellingShingle |
GPI Trans-sialidase Trypanosoma cruzi lipid palmitic acid sialidase article immunoprecipitation nonhuman priority journal thin layer chromatography trypanosoma cruzi Animals Chromatography, Thin Layer Glycosylphosphatidylinositols Membrane Lipids Mice Neuraminidase Phosphatidylinositol Diacylglycerol-Lyase Phospholipase C Substrate Specificity Trypanosoma cruzi Trypanosoma Trypanosoma cruzi The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids |
| topic_facet |
GPI Trans-sialidase Trypanosoma cruzi lipid palmitic acid sialidase article immunoprecipitation nonhuman priority journal thin layer chromatography trypanosoma cruzi Animals Chromatography, Thin Layer Glycosylphosphatidylinositols Membrane Lipids Mice Neuraminidase Phosphatidylinositol Diacylglycerol-Lyase Phospholipase C Substrate Specificity Trypanosoma cruzi Trypanosoma Trypanosoma cruzi |
| description |
The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-h exadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T. ruzi was made. |
| title |
The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids |
| title_short |
The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids |
| title_full |
The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids |
| title_fullStr |
The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids |
| title_full_unstemmed |
The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids |
| title_sort |
trans-sialidase of trypanosoma cruzi is anchored by two different lipids |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09596658_v7_n6_p731_Agusti http://hdl.handle.net/20.500.12110/paper_09596658_v7_n6_p731_Agusti |
| _version_ |
1840326082464579584 |