Dexamethasone up-regulates mRNA for Na+,K+-ATPase in some spinal cord neurones after cord transection

We have studied the effects of spinal cord transection and of dexamethasone (DEX) treatment on mRNA biosynthesis of the Na+,K+-ATPase, a key enzyme necessary for neurotransmission, membrane repolarization and nutrient uptake in the CNS. In situ hybridization analysis revealed a significant reduction...

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Guardado en:
Detalles Bibliográficos
Publicado: 1996
Materias:
CNS injury
Dexamethasone
Glucocorticoid
In situ hybridization
Na+,K+-ATPase
Spinal cord
adenosine triphosphatase (potassium sodium)
dexamethasone
messenger RNA
animal model
animal tissue
article
controlled study
enzyme active site
in situ hybridization
male
messenger RNA synthesis
nonhuman
priority journal
rat
spinal cord injury
spinal cord nerve cell
spinal cord transsection
spinal cord ventral horn
subcutaneous drug administration
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v7_n5_p1041_Gonzalez
http://hdl.handle.net/20.500.12110/paper_09594965_v7_n5_p1041_Gonzalez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:We have studied the effects of spinal cord transection and of dexamethasone (DEX) treatment on mRNA biosynthesis of the Na+,K+-ATPase, a key enzyme necessary for neurotransmission, membrane repolarization and nutrient uptake in the CNS. In situ hybridization analysis revealed a significant reduction in mRNA for the α3 catalytic subunit of the enzyme in medium and large size ventral horn neurones (1000-2300 μm2) but not in small cells (1000 μm2) 24 h after spinal cord transection. DEX treatment significantly reversed the transection effect in medium and large size neurones. It is suggested that up-regulation of mRNA expression for Na+,K+- ATPase may constitute an important mechanism by which glucocorticoids help to re-establish neuronal function after spinal cord injury.

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