Dexamethasone up-regulates mRNA for Na+,K+-ATPase in some spinal cord neurones after cord transection
We have studied the effects of spinal cord transection and of dexamethasone (DEX) treatment on mRNA biosynthesis of the Na+,K+-ATPase, a key enzyme necessary for neurotransmission, membrane repolarization and nutrient uptake in the CNS. In situ hybridization analysis revealed a significant reduction...
Guardado en:
| Publicado: |
1996
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v7_n5_p1041_Gonzalez http://hdl.handle.net/20.500.12110/paper_09594965_v7_n5_p1041_Gonzalez |
| Aporte de: |
| id |
paper:paper_09594965_v7_n5_p1041_Gonzalez |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_09594965_v7_n5_p1041_Gonzalez2023-06-08T15:57:00Z Dexamethasone up-regulates mRNA for Na+,K+-ATPase in some spinal cord neurones after cord transection CNS injury Dexamethasone Glucocorticoid In situ hybridization Na+,K+-ATPase Spinal cord adenosine triphosphatase (potassium sodium) dexamethasone messenger RNA animal model animal tissue article controlled study enzyme active site in situ hybridization male messenger RNA synthesis nonhuman priority journal rat spinal cord injury spinal cord nerve cell spinal cord transsection spinal cord ventral horn subcutaneous drug administration We have studied the effects of spinal cord transection and of dexamethasone (DEX) treatment on mRNA biosynthesis of the Na+,K+-ATPase, a key enzyme necessary for neurotransmission, membrane repolarization and nutrient uptake in the CNS. In situ hybridization analysis revealed a significant reduction in mRNA for the α3 catalytic subunit of the enzyme in medium and large size ventral horn neurones (1000-2300 μm2) but not in small cells (1000 μm2) 24 h after spinal cord transection. DEX treatment significantly reversed the transection effect in medium and large size neurones. It is suggested that up-regulation of mRNA expression for Na+,K+- ATPase may constitute an important mechanism by which glucocorticoids help to re-establish neuronal function after spinal cord injury. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v7_n5_p1041_Gonzalez http://hdl.handle.net/20.500.12110/paper_09594965_v7_n5_p1041_Gonzalez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CNS injury Dexamethasone Glucocorticoid In situ hybridization Na+,K+-ATPase Spinal cord adenosine triphosphatase (potassium sodium) dexamethasone messenger RNA animal model animal tissue article controlled study enzyme active site in situ hybridization male messenger RNA synthesis nonhuman priority journal rat spinal cord injury spinal cord nerve cell spinal cord transsection spinal cord ventral horn subcutaneous drug administration |
| spellingShingle |
CNS injury Dexamethasone Glucocorticoid In situ hybridization Na+,K+-ATPase Spinal cord adenosine triphosphatase (potassium sodium) dexamethasone messenger RNA animal model animal tissue article controlled study enzyme active site in situ hybridization male messenger RNA synthesis nonhuman priority journal rat spinal cord injury spinal cord nerve cell spinal cord transsection spinal cord ventral horn subcutaneous drug administration Dexamethasone up-regulates mRNA for Na+,K+-ATPase in some spinal cord neurones after cord transection |
| topic_facet |
CNS injury Dexamethasone Glucocorticoid In situ hybridization Na+,K+-ATPase Spinal cord adenosine triphosphatase (potassium sodium) dexamethasone messenger RNA animal model animal tissue article controlled study enzyme active site in situ hybridization male messenger RNA synthesis nonhuman priority journal rat spinal cord injury spinal cord nerve cell spinal cord transsection spinal cord ventral horn subcutaneous drug administration |
| description |
We have studied the effects of spinal cord transection and of dexamethasone (DEX) treatment on mRNA biosynthesis of the Na+,K+-ATPase, a key enzyme necessary for neurotransmission, membrane repolarization and nutrient uptake in the CNS. In situ hybridization analysis revealed a significant reduction in mRNA for the α3 catalytic subunit of the enzyme in medium and large size ventral horn neurones (1000-2300 μm2) but not in small cells (1000 μm2) 24 h after spinal cord transection. DEX treatment significantly reversed the transection effect in medium and large size neurones. It is suggested that up-regulation of mRNA expression for Na+,K+- ATPase may constitute an important mechanism by which glucocorticoids help to re-establish neuronal function after spinal cord injury. |
| title |
Dexamethasone up-regulates mRNA for Na+,K+-ATPase in some spinal cord neurones after cord transection |
| title_short |
Dexamethasone up-regulates mRNA for Na+,K+-ATPase in some spinal cord neurones after cord transection |
| title_full |
Dexamethasone up-regulates mRNA for Na+,K+-ATPase in some spinal cord neurones after cord transection |
| title_fullStr |
Dexamethasone up-regulates mRNA for Na+,K+-ATPase in some spinal cord neurones after cord transection |
| title_full_unstemmed |
Dexamethasone up-regulates mRNA for Na+,K+-ATPase in some spinal cord neurones after cord transection |
| title_sort |
dexamethasone up-regulates mrna for na+,k+-atpase in some spinal cord neurones after cord transection |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09594965_v7_n5_p1041_Gonzalez http://hdl.handle.net/20.500.12110/paper_09594965_v7_n5_p1041_Gonzalez |
| _version_ |
1768543239019692032 |