Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae

The aim of this work was to determine the in vitro effect of the mixture between the lipopeptide surfactin, synthesized by Bacillus subtilis C4 (strain isolated from honey) and the most active vegetal extract from Achyrocline satureioides, a traditional medicinal plant, on local strains of Paenibaci...

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Publicado:	2012
Materias:	Achyrocline satureioides Antimicrobial Paenibacillus larvae Surfactin Synergism Benzene Hexane Microorganisms Plant extracts Strain Surface active agents Essential oils antiinfective agent cyclopeptide lipopeptide plant extract surfactin peptide Achyrocline Argentina article Bacillus subtilis chemistry drug effect drug potentiation isolation and purification microbial sensitivity test microbial viability Paenibacillus physiology Anti-Bacterial Agents Drug Synergism Lipopeptides Microbial Sensitivity Tests Microbial Viability Peptides, Cyclic Plant Extracts Apis mellifera
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n4_p1415_Sabate http://hdl.handle.net/20.500.12110/paper_09593993_v28_n4_p1415_Sabate
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09593993_v28_n4_p1415_Sabate
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spelling	paper:paper_09593993_v28_n4_p1415_Sabate2023-06-08T15:56:54Z Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae Achyrocline satureioides Antimicrobial Paenibacillus larvae Surfactin Synergism Achyrocline satureioides Antimicrobial Paenibacillus larvae Surfactin Synergism Benzene Hexane Microorganisms Plant extracts Strain Surface active agents Essential oils antiinfective agent cyclopeptide lipopeptide plant extract surfactin peptide Achyrocline Argentina article Bacillus subtilis chemistry drug effect drug potentiation isolation and purification microbial sensitivity test microbial viability Paenibacillus physiology Achyrocline Anti-Bacterial Agents Argentina Bacillus subtilis Drug Synergism Lipopeptides Microbial Sensitivity Tests Microbial Viability Paenibacillus Peptides, Cyclic Plant Extracts Achyrocline Achyrocline satureioides Apis mellifera Bacillus subtilis Paenibacillus Paenibacillus larvae The aim of this work was to determine the in vitro effect of the mixture between the lipopeptide surfactin, synthesized by Bacillus subtilis C4 (strain isolated from honey) and the most active vegetal extract from Achyrocline satureioides, a traditional medicinal plant, on local strains of Paenibacillus larvae, the agent of American Foulbrood in honeybees. Five P. larvae strains isolated in Córdoba, Argentina, were phenotypically characterized. These and 12 other P. larvae strains from different regions of Argentina were analysed. The antimicrobial activities of the essential oil, hexane (HE) and benzene extracts from A. satureioides were assessed against P. larvae and the HE showed the highest anti-P. larvae activity. A combination of the biosurfactant surfactin, produced by B. subtilis C4, and the HE of A. satureioides revealed a synergistic action on P. larvae. The effective surfactin concentration in the mixture decreased from 32 to 1 μg ml-1 and the HE concentration from 32 to 4 μg ml-1, values similar or equal to minimal inhibitory concentrations observed for oxytetracycline. The fractional inhibitory concentration index confirmed synergism in 4 strains and partial synergism in one strain. The combination of surfactin synthesized by B. subtilis C4 and the HE from A. satureioides could be a natural alternative to help beekeepers to combat the American foulbrood agent P. larvae. © 2011 Springer Science+Business Media B.V. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n4_p1415_Sabate http://hdl.handle.net/20.500.12110/paper_09593993_v28_n4_p1415_Sabate
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Achyrocline satureioides Antimicrobial Paenibacillus larvae Surfactin Synergism Achyrocline satureioides Antimicrobial Paenibacillus larvae Surfactin Synergism Benzene Hexane Microorganisms Plant extracts Strain Surface active agents Essential oils antiinfective agent cyclopeptide lipopeptide plant extract surfactin peptide Achyrocline Argentina article Bacillus subtilis chemistry drug effect drug potentiation isolation and purification microbial sensitivity test microbial viability Paenibacillus physiology Achyrocline Anti-Bacterial Agents Argentina Bacillus subtilis Drug Synergism Lipopeptides Microbial Sensitivity Tests Microbial Viability Paenibacillus Peptides, Cyclic Plant Extracts Achyrocline Achyrocline satureioides Apis mellifera Bacillus subtilis Paenibacillus Paenibacillus larvae
spellingShingle	Achyrocline satureioides Antimicrobial Paenibacillus larvae Surfactin Synergism Achyrocline satureioides Antimicrobial Paenibacillus larvae Surfactin Synergism Benzene Hexane Microorganisms Plant extracts Strain Surface active agents Essential oils antiinfective agent cyclopeptide lipopeptide plant extract surfactin peptide Achyrocline Argentina article Bacillus subtilis chemistry drug effect drug potentiation isolation and purification microbial sensitivity test microbial viability Paenibacillus physiology Achyrocline Anti-Bacterial Agents Argentina Bacillus subtilis Drug Synergism Lipopeptides Microbial Sensitivity Tests Microbial Viability Paenibacillus Peptides, Cyclic Plant Extracts Achyrocline Achyrocline satureioides Apis mellifera Bacillus subtilis Paenibacillus Paenibacillus larvae Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
topic_facet	Achyrocline satureioides Antimicrobial Paenibacillus larvae Surfactin Synergism Achyrocline satureioides Antimicrobial Paenibacillus larvae Surfactin Synergism Benzene Hexane Microorganisms Plant extracts Strain Surface active agents Essential oils antiinfective agent cyclopeptide lipopeptide plant extract surfactin peptide Achyrocline Argentina article Bacillus subtilis chemistry drug effect drug potentiation isolation and purification microbial sensitivity test microbial viability Paenibacillus physiology Achyrocline Anti-Bacterial Agents Argentina Bacillus subtilis Drug Synergism Lipopeptides Microbial Sensitivity Tests Microbial Viability Paenibacillus Peptides, Cyclic Plant Extracts Achyrocline Achyrocline satureioides Apis mellifera Bacillus subtilis Paenibacillus Paenibacillus larvae
description	The aim of this work was to determine the in vitro effect of the mixture between the lipopeptide surfactin, synthesized by Bacillus subtilis C4 (strain isolated from honey) and the most active vegetal extract from Achyrocline satureioides, a traditional medicinal plant, on local strains of Paenibacillus larvae, the agent of American Foulbrood in honeybees. Five P. larvae strains isolated in Córdoba, Argentina, were phenotypically characterized. These and 12 other P. larvae strains from different regions of Argentina were analysed. The antimicrobial activities of the essential oil, hexane (HE) and benzene extracts from A. satureioides were assessed against P. larvae and the HE showed the highest anti-P. larvae activity. A combination of the biosurfactant surfactin, produced by B. subtilis C4, and the HE of A. satureioides revealed a synergistic action on P. larvae. The effective surfactin concentration in the mixture decreased from 32 to 1 μg ml-1 and the HE concentration from 32 to 4 μg ml-1, values similar or equal to minimal inhibitory concentrations observed for oxytetracycline. The fractional inhibitory concentration index confirmed synergism in 4 strains and partial synergism in one strain. The combination of surfactin synthesized by B. subtilis C4 and the HE from A. satureioides could be a natural alternative to help beekeepers to combat the American foulbrood agent P. larvae. © 2011 Springer Science+Business Media B.V.
title	Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
title_short	Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
title_full	Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
title_fullStr	Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
title_full_unstemmed	Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae
title_sort	synergistic effect of surfactin from bacillus subtilis c4 and achyrocline satureioides extracts on the viability of paenibacillus larvae
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09593993_v28_n4_p1415_Sabate http://hdl.handle.net/20.500.12110/paper_09593993_v28_n4_p1415_Sabate
_version_	1768543381962620928

Synergistic effect of surfactin from Bacillus subtilis C4 and Achyrocline satureioides extracts on the viability of Paenibacillus larvae

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