Validation of an enzyme immunoassay for the determination of total homocysteine in plasma
In recent years, the determination of homocysteine (Hcy) has become increasingly important, since high levels of Hcy in plasma or serum represent an independent risk factor for occlusive vascular diseases. Nowadays, clinical laboratories use several analytical techniques to measure Hcy, of which hig...
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2000
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09575235_v11_n3_p235_Quintana http://hdl.handle.net/20.500.12110/paper_09575235_v11_n3_p235_Quintana |
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paper:paper_09575235_v11_n3_p235_Quintana2023-06-08T15:56:37Z Validation of an enzyme immunoassay for the determination of total homocysteine in plasma Enzyme immunoassay Homocysteine Validation homocysteine article enzyme immunoassay high performance liquid chromatography human human experiment intermethod comparison normal human priority journal Homocysteine Humans Immunoenzyme Techniques Sensitivity and Specificity In recent years, the determination of homocysteine (Hcy) has become increasingly important, since high levels of Hcy in plasma or serum represent an independent risk factor for occlusive vascular diseases. Nowadays, clinical laboratories use several analytical techniques to measure Hcy, of which high-performance liquid chromatography (HPLC) is the most popular. Recently, assays for Hcy quantification based on enzyme immunoassays (EIA) have become commercially available. Our group carried out the validation of the Axis method and compared results with those obtained by an established HPLC assay. Intra- and inter-assay coefficients of variation were ≤ 8.5%. Compared with HPLC, linear regression analysis showed r = 0.984, slope = 0.952, intercept = 1.24 μmol/l; Bland-Altman procedure, the mean of the difference EIA-HPLC results = 0.5 μmol/l. Our results suggest that Hcy determinations by both methods are equivalent, and that the Axis assay provides reproducible and reliable data. (C) 2000 Lippincott Williams and Wilkins. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09575235_v11_n3_p235_Quintana http://hdl.handle.net/20.500.12110/paper_09575235_v11_n3_p235_Quintana |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Enzyme immunoassay Homocysteine Validation homocysteine article enzyme immunoassay high performance liquid chromatography human human experiment intermethod comparison normal human priority journal Homocysteine Humans Immunoenzyme Techniques Sensitivity and Specificity |
| spellingShingle |
Enzyme immunoassay Homocysteine Validation homocysteine article enzyme immunoassay high performance liquid chromatography human human experiment intermethod comparison normal human priority journal Homocysteine Humans Immunoenzyme Techniques Sensitivity and Specificity Validation of an enzyme immunoassay for the determination of total homocysteine in plasma |
| topic_facet |
Enzyme immunoassay Homocysteine Validation homocysteine article enzyme immunoassay high performance liquid chromatography human human experiment intermethod comparison normal human priority journal Homocysteine Humans Immunoenzyme Techniques Sensitivity and Specificity |
| description |
In recent years, the determination of homocysteine (Hcy) has become increasingly important, since high levels of Hcy in plasma or serum represent an independent risk factor for occlusive vascular diseases. Nowadays, clinical laboratories use several analytical techniques to measure Hcy, of which high-performance liquid chromatography (HPLC) is the most popular. Recently, assays for Hcy quantification based on enzyme immunoassays (EIA) have become commercially available. Our group carried out the validation of the Axis method and compared results with those obtained by an established HPLC assay. Intra- and inter-assay coefficients of variation were ≤ 8.5%. Compared with HPLC, linear regression analysis showed r = 0.984, slope = 0.952, intercept = 1.24 μmol/l; Bland-Altman procedure, the mean of the difference EIA-HPLC results = 0.5 μmol/l. Our results suggest that Hcy determinations by both methods are equivalent, and that the Axis assay provides reproducible and reliable data. (C) 2000 Lippincott Williams and Wilkins. |
| title |
Validation of an enzyme immunoassay for the determination of total homocysteine in plasma |
| title_short |
Validation of an enzyme immunoassay for the determination of total homocysteine in plasma |
| title_full |
Validation of an enzyme immunoassay for the determination of total homocysteine in plasma |
| title_fullStr |
Validation of an enzyme immunoassay for the determination of total homocysteine in plasma |
| title_full_unstemmed |
Validation of an enzyme immunoassay for the determination of total homocysteine in plasma |
| title_sort |
validation of an enzyme immunoassay for the determination of total homocysteine in plasma |
| publishDate |
2000 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09575235_v11_n3_p235_Quintana http://hdl.handle.net/20.500.12110/paper_09575235_v11_n3_p235_Quintana |
| _version_ |
1768545984202145792 |