Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture

Toxigenic Aspergillus flavus and A. parasiticus fungal strains can contaminate a wide variety of food crops with the subsequent production of aflatoxins (AFs) resulting in severe economic losses and public health issues. Biological control is a promising approach to manage AFs contamination in pre-...

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Detalles Bibliográficos
Publicado: 2018
Materias:
Aflatoxin B1
Aspergillus parasiticus
Bacillus
Lipopeptides
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09567135_v85_n_p392_GonzalezPereyra
http://hdl.handle.net/20.500.12110/paper_09567135_v85_n_p392_GonzalezPereyra
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09567135_v85_n_p392_GonzalezPereyra
record_format dspace
spelling paper:paper_09567135_v85_n_p392_GonzalezPereyra2023-06-08T15:56:22Z Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture Aflatoxin B1 Aspergillus parasiticus Bacillus Lipopeptides Toxigenic Aspergillus flavus and A. parasiticus fungal strains can contaminate a wide variety of food crops with the subsequent production of aflatoxins (AFs) resulting in severe economic losses and public health issues. Biological control is a promising approach to manage AFs contamination in pre- and post-harvested crops. In the present study, the effect of soil-borne Bacillus spp. strains on aflatoxigenic A. parasiticus growth and AFs production was evaluated and the culture supernatant of the most effective strain was evaluated for the presence of antifungal lipopeptides. Six Bacillus spp. strains were able to reduce A. parasiticus growth rate significantly (p < 0.05). Bacillus spp. RC1A was able to inhibit fungal growth almost completely, reducing growth rate to 0.16 mm/h and increasing Lag phase duration (31.72 h) (p < 0.0001). RC1A could also reduce AFB1 concentration produced by A. parasiticus (p < 0.0001). Organic solvent extraction and chromatographic analysis of RC1A culture supernatant showed the presence of bands corresponding to three of the main groups of lipopeptides (surfactin, iturin A and fengycin) at the expected retention factor (Rf) values; they were also confirmed by MALDI-MS analysis. These fractions were able to inhibit A. parasiticus growth and AFB1 production to non-detectable levels when tested separately in liquid culture media. The further study of the antifungal compounds produced by these strains will determine their potential use to manage AFs contamination in crops and feeds. © 2017 Elsevier Ltd 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09567135_v85_n_p392_GonzalezPereyra http://hdl.handle.net/20.500.12110/paper_09567135_v85_n_p392_GonzalezPereyra
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Aflatoxin B1
Aspergillus parasiticus
Bacillus
Lipopeptides
spellingShingle Aflatoxin B1
Aspergillus parasiticus
Bacillus
Lipopeptides
Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
topic_facet Aflatoxin B1
Aspergillus parasiticus
Bacillus
Lipopeptides
description Toxigenic Aspergillus flavus and A. parasiticus fungal strains can contaminate a wide variety of food crops with the subsequent production of aflatoxins (AFs) resulting in severe economic losses and public health issues. Biological control is a promising approach to manage AFs contamination in pre- and post-harvested crops. In the present study, the effect of soil-borne Bacillus spp. strains on aflatoxigenic A. parasiticus growth and AFs production was evaluated and the culture supernatant of the most effective strain was evaluated for the presence of antifungal lipopeptides. Six Bacillus spp. strains were able to reduce A. parasiticus growth rate significantly (p < 0.05). Bacillus spp. RC1A was able to inhibit fungal growth almost completely, reducing growth rate to 0.16 mm/h and increasing Lag phase duration (31.72 h) (p < 0.0001). RC1A could also reduce AFB1 concentration produced by A. parasiticus (p < 0.0001). Organic solvent extraction and chromatographic analysis of RC1A culture supernatant showed the presence of bands corresponding to three of the main groups of lipopeptides (surfactin, iturin A and fengycin) at the expected retention factor (Rf) values; they were also confirmed by MALDI-MS analysis. These fractions were able to inhibit A. parasiticus growth and AFB1 production to non-detectable levels when tested separately in liquid culture media. The further study of the antifungal compounds produced by these strains will determine their potential use to manage AFs contamination in crops and feeds. © 2017 Elsevier Ltd
title Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
title_short Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
title_full Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
title_fullStr Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
title_full_unstemmed Antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil Bacillus strains with potential application in agriculture
title_sort antifungal and aflatoxin-reducing activity of extracellular compounds produced by soil bacillus strains with potential application in agriculture
publishDate 2018
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09567135_v85_n_p392_GonzalezPereyra
http://hdl.handle.net/20.500.12110/paper_09567135_v85_n_p392_GonzalezPereyra
_version_ 1768546031237070848

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