Obesity alters the uterine environment before pregnancy
Obesity is a metabolic disorder that predisposes to numerous diseases and has become a major global public health concern. Cafeteria diet (CAF) is the animal model used for the study of obesity that more closely reflects Western diet habits. Previously, we described that CAF administration for 60 da...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09552863_v62_n_p181_Bazzano http://hdl.handle.net/20.500.12110/paper_09552863_v62_n_p181_Bazzano |
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paper:paper_09552863_v62_n_p181_Bazzano2023-06-08T15:55:53Z Obesity alters the uterine environment before pregnancy Adrenergic receptor Glucose Insulin Obesity Uterine contractile activity Uterus beta 2 adrenergic receptor insulin receptor salbutamol abdominal circumference animal experiment Article body weight gain cell proliferation controlled study down regulation female fetus weight glucose transport immunofluorescence immunohistochemistry insulin resistance macrosomia myometrium nidation nonhuman obesity polymerase chain reaction pregnancy pregnancy rate protein expression rat real time polymerase chain reaction RNA extraction uterus uterus contraction Western blotting Obesity is a metabolic disorder that predisposes to numerous diseases and has become a major global public health concern. Cafeteria diet (CAF) is the animal model used for the study of obesity that more closely reflects Western diet habits. Previously, we described that CAF administration for 60 days induces obesity in female rats and their fetuses develop macrosomia. Given that, in our model, rats are not genetically modified and that obese mothers were fed standard chow during pregnancy, the aim of the current study was to test the hypothesis that obesity alters the intrauterine environment prior to pregnancy, and this may explain the exacerbated fetal weight gain. We found that uteri from obese rats during the estrous phase developed insulin resistance through mechanisms that involve the induction of uterine hypoxia and the down-regulation of the insulin receptor gene. Moreover, uterine cell proliferation was induced by obesity concomitantly with the reduction in the uterine contractile response to a β2 AR agonist, salbutamol, and this may be consequence of the down-regulation in the uterine β2 AR expression. We conclude that CAF-induced obesity alters the uterine environment in rats during the estrous phase and may cause the fetal macrosomia previously described by us in obese animals. The lower sensitivity of the uterus to a relaxation stimulus (salbutamol) is not a minor fact given that for implantation to occur the uterus must be relaxed for embryo nidation. Thus, the alteration in the uterine quiescence may impair implantation and, consequently, the foregoing pregnancy. © 2018 Elsevier Inc. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09552863_v62_n_p181_Bazzano http://hdl.handle.net/20.500.12110/paper_09552863_v62_n_p181_Bazzano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adrenergic receptor Glucose Insulin Obesity Uterine contractile activity Uterus beta 2 adrenergic receptor insulin receptor salbutamol abdominal circumference animal experiment Article body weight gain cell proliferation controlled study down regulation female fetus weight glucose transport immunofluorescence immunohistochemistry insulin resistance macrosomia myometrium nidation nonhuman obesity polymerase chain reaction pregnancy pregnancy rate protein expression rat real time polymerase chain reaction RNA extraction uterus uterus contraction Western blotting |
| spellingShingle |
Adrenergic receptor Glucose Insulin Obesity Uterine contractile activity Uterus beta 2 adrenergic receptor insulin receptor salbutamol abdominal circumference animal experiment Article body weight gain cell proliferation controlled study down regulation female fetus weight glucose transport immunofluorescence immunohistochemistry insulin resistance macrosomia myometrium nidation nonhuman obesity polymerase chain reaction pregnancy pregnancy rate protein expression rat real time polymerase chain reaction RNA extraction uterus uterus contraction Western blotting Obesity alters the uterine environment before pregnancy |
| topic_facet |
Adrenergic receptor Glucose Insulin Obesity Uterine contractile activity Uterus beta 2 adrenergic receptor insulin receptor salbutamol abdominal circumference animal experiment Article body weight gain cell proliferation controlled study down regulation female fetus weight glucose transport immunofluorescence immunohistochemistry insulin resistance macrosomia myometrium nidation nonhuman obesity polymerase chain reaction pregnancy pregnancy rate protein expression rat real time polymerase chain reaction RNA extraction uterus uterus contraction Western blotting |
| description |
Obesity is a metabolic disorder that predisposes to numerous diseases and has become a major global public health concern. Cafeteria diet (CAF) is the animal model used for the study of obesity that more closely reflects Western diet habits. Previously, we described that CAF administration for 60 days induces obesity in female rats and their fetuses develop macrosomia. Given that, in our model, rats are not genetically modified and that obese mothers were fed standard chow during pregnancy, the aim of the current study was to test the hypothesis that obesity alters the intrauterine environment prior to pregnancy, and this may explain the exacerbated fetal weight gain. We found that uteri from obese rats during the estrous phase developed insulin resistance through mechanisms that involve the induction of uterine hypoxia and the down-regulation of the insulin receptor gene. Moreover, uterine cell proliferation was induced by obesity concomitantly with the reduction in the uterine contractile response to a β2 AR agonist, salbutamol, and this may be consequence of the down-regulation in the uterine β2 AR expression. We conclude that CAF-induced obesity alters the uterine environment in rats during the estrous phase and may cause the fetal macrosomia previously described by us in obese animals. The lower sensitivity of the uterus to a relaxation stimulus (salbutamol) is not a minor fact given that for implantation to occur the uterus must be relaxed for embryo nidation. Thus, the alteration in the uterine quiescence may impair implantation and, consequently, the foregoing pregnancy. © 2018 Elsevier Inc. |
| title |
Obesity alters the uterine environment before pregnancy |
| title_short |
Obesity alters the uterine environment before pregnancy |
| title_full |
Obesity alters the uterine environment before pregnancy |
| title_fullStr |
Obesity alters the uterine environment before pregnancy |
| title_full_unstemmed |
Obesity alters the uterine environment before pregnancy |
| title_sort |
obesity alters the uterine environment before pregnancy |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09552863_v62_n_p181_Bazzano http://hdl.handle.net/20.500.12110/paper_09552863_v62_n_p181_Bazzano |
| _version_ |
1768545101819150336 |