Obesity alters the ovarian glucidic homeostasis disrupting the reproductive outcome of female rats
Obesity constitutes a health problem of increasing worldwide prevalence related to many reproductive problems such as infertility, ovulation dysfunction, preterm delivery, fetal growth disorders, etc. The mechanisms linking obesity to these pathologies are not fully understood. Cafeteria diet (CAF)...
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paper:paper_09552863_v42_n_p194_Bazzano2023-06-08T15:55:53Z Obesity alters the ovarian glucidic homeostasis disrupting the reproductive outcome of female rats Paz, Dante Agustin Elia, Evelin Mariel Fertility Glucose Glut-4 Insulin Obesity Ovary Reproductive outcome glucose glucose transporter 4 insulin insulin receptor glucose transporter 4 insulin receptor nitric oxide synthase Slc2a4 protein, rat animal experiment animal tissue Article body fat distribution cafeteria diet controlled study diet diet induced obesity female gene expression glucose intolerance glucose tolerance test glucose transport homeostasis hyperglycemia immunohistochemistry insulin resistance insulin tolerance test macrosomia nonhuman obesity ovarian glucidic homeostasis ovary rat reproduction reproductive success animal complication genetics homeostasis metabolism obesity ovary follicle pathology pathophysiology pregnancy Wistar rat Animals Body Fat Distribution Diet Female Glucose Tolerance Test Glucose Transporter Type 4 Homeostasis Insulin Resistance Nitric Oxide Synthase Obesity Ovarian Follicle Ovary Pregnancy Rats, Wistar Receptor, Insulin Obesity constitutes a health problem of increasing worldwide prevalence related to many reproductive problems such as infertility, ovulation dysfunction, preterm delivery, fetal growth disorders, etc. The mechanisms linking obesity to these pathologies are not fully understood. Cafeteria diet (CAF) is the animal model used for the study of obesity that more closely reflects western diet habits. Previously we described that CAF induces obesity associated to hyperglycemia, reduced ovarian reserve, presence of follicular cysts and ovulatory impairments. The aim of the present study was to contribute in the understanding of the physiological mechanisms altered as consequence of obesity. For that purpose, female Wistar rats were fed ad libitum with a standard diet (control group) or CAF (Obese group). We found that CAF fed-rats developed obesity, glucose intolerance and insulin resistance. Ovaries from obese rats showed decreased glucose uptake and became insulin resistant, showing decreased ovarian expression of glucotransporter type 4 and insulin receptor gene expression respect to controls. These animals showed an increased follicular nitric oxyde synthase expression that may be responsible for the ovulatory disruptions and for inflammation, a common feature in obesity. Obese rats resulted subfertile and their pups were macrosomic. We conclude that obesity alters the systemic and the ovarian glucidic homeostasis impairing the reproductive outcome. Since macrosomia is a risk factor for metabolic and obstetric disorders in adult life, we suggest that obesity is impacting not only on health and reproduction but it is also impacting on health and reproduction of the offspring. © 2017 Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Elia, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09552863_v42_n_p194_Bazzano http://hdl.handle.net/20.500.12110/paper_09552863_v42_n_p194_Bazzano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fertility Glucose Glut-4 Insulin Obesity Ovary Reproductive outcome glucose glucose transporter 4 insulin insulin receptor glucose transporter 4 insulin receptor nitric oxide synthase Slc2a4 protein, rat animal experiment animal tissue Article body fat distribution cafeteria diet controlled study diet diet induced obesity female gene expression glucose intolerance glucose tolerance test glucose transport homeostasis hyperglycemia immunohistochemistry insulin resistance insulin tolerance test macrosomia nonhuman obesity ovarian glucidic homeostasis ovary rat reproduction reproductive success animal complication genetics homeostasis metabolism obesity ovary follicle pathology pathophysiology pregnancy Wistar rat Animals Body Fat Distribution Diet Female Glucose Tolerance Test Glucose Transporter Type 4 Homeostasis Insulin Resistance Nitric Oxide Synthase Obesity Ovarian Follicle Ovary Pregnancy Rats, Wistar Receptor, Insulin |
| spellingShingle |
Fertility Glucose Glut-4 Insulin Obesity Ovary Reproductive outcome glucose glucose transporter 4 insulin insulin receptor glucose transporter 4 insulin receptor nitric oxide synthase Slc2a4 protein, rat animal experiment animal tissue Article body fat distribution cafeteria diet controlled study diet diet induced obesity female gene expression glucose intolerance glucose tolerance test glucose transport homeostasis hyperglycemia immunohistochemistry insulin resistance insulin tolerance test macrosomia nonhuman obesity ovarian glucidic homeostasis ovary rat reproduction reproductive success animal complication genetics homeostasis metabolism obesity ovary follicle pathology pathophysiology pregnancy Wistar rat Animals Body Fat Distribution Diet Female Glucose Tolerance Test Glucose Transporter Type 4 Homeostasis Insulin Resistance Nitric Oxide Synthase Obesity Ovarian Follicle Ovary Pregnancy Rats, Wistar Receptor, Insulin Paz, Dante Agustin Elia, Evelin Mariel Obesity alters the ovarian glucidic homeostasis disrupting the reproductive outcome of female rats |
| topic_facet |
Fertility Glucose Glut-4 Insulin Obesity Ovary Reproductive outcome glucose glucose transporter 4 insulin insulin receptor glucose transporter 4 insulin receptor nitric oxide synthase Slc2a4 protein, rat animal experiment animal tissue Article body fat distribution cafeteria diet controlled study diet diet induced obesity female gene expression glucose intolerance glucose tolerance test glucose transport homeostasis hyperglycemia immunohistochemistry insulin resistance insulin tolerance test macrosomia nonhuman obesity ovarian glucidic homeostasis ovary rat reproduction reproductive success animal complication genetics homeostasis metabolism obesity ovary follicle pathology pathophysiology pregnancy Wistar rat Animals Body Fat Distribution Diet Female Glucose Tolerance Test Glucose Transporter Type 4 Homeostasis Insulin Resistance Nitric Oxide Synthase Obesity Ovarian Follicle Ovary Pregnancy Rats, Wistar Receptor, Insulin |
| description |
Obesity constitutes a health problem of increasing worldwide prevalence related to many reproductive problems such as infertility, ovulation dysfunction, preterm delivery, fetal growth disorders, etc. The mechanisms linking obesity to these pathologies are not fully understood. Cafeteria diet (CAF) is the animal model used for the study of obesity that more closely reflects western diet habits. Previously we described that CAF induces obesity associated to hyperglycemia, reduced ovarian reserve, presence of follicular cysts and ovulatory impairments. The aim of the present study was to contribute in the understanding of the physiological mechanisms altered as consequence of obesity. For that purpose, female Wistar rats were fed ad libitum with a standard diet (control group) or CAF (Obese group). We found that CAF fed-rats developed obesity, glucose intolerance and insulin resistance. Ovaries from obese rats showed decreased glucose uptake and became insulin resistant, showing decreased ovarian expression of glucotransporter type 4 and insulin receptor gene expression respect to controls. These animals showed an increased follicular nitric oxyde synthase expression that may be responsible for the ovulatory disruptions and for inflammation, a common feature in obesity. Obese rats resulted subfertile and their pups were macrosomic. We conclude that obesity alters the systemic and the ovarian glucidic homeostasis impairing the reproductive outcome. Since macrosomia is a risk factor for metabolic and obstetric disorders in adult life, we suggest that obesity is impacting not only on health and reproduction but it is also impacting on health and reproduction of the offspring. © 2017 |
| author |
Paz, Dante Agustin Elia, Evelin Mariel |
| author_facet |
Paz, Dante Agustin Elia, Evelin Mariel |
| author_sort |
Paz, Dante Agustin |
| title |
Obesity alters the ovarian glucidic homeostasis disrupting the reproductive outcome of female rats |
| title_short |
Obesity alters the ovarian glucidic homeostasis disrupting the reproductive outcome of female rats |
| title_full |
Obesity alters the ovarian glucidic homeostasis disrupting the reproductive outcome of female rats |
| title_fullStr |
Obesity alters the ovarian glucidic homeostasis disrupting the reproductive outcome of female rats |
| title_full_unstemmed |
Obesity alters the ovarian glucidic homeostasis disrupting the reproductive outcome of female rats |
| title_sort |
obesity alters the ovarian glucidic homeostasis disrupting the reproductive outcome of female rats |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09552863_v42_n_p194_Bazzano http://hdl.handle.net/20.500.12110/paper_09552863_v42_n_p194_Bazzano |
| work_keys_str_mv |
AT pazdanteagustin obesityalterstheovarianglucidichomeostasisdisruptingthereproductiveoutcomeoffemalerats AT eliaevelinmariel obesityalterstheovarianglucidichomeostasisdisruptingthereproductiveoutcomeoffemalerats |
| _version_ |
1768545518940585984 |