Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone

The role of gonadotrophin-inhibitory hormone (GnIH) in the inhibition of the reproductive axis has been well-established in birds and mammals. However, its role in other vertebrates, such as the teleost fish, remains controversial. In this context, the present study aimed to evaluate whether GnIH mo...

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Autores principales:	Di Yorio, María Paula, Somoza, Gustavo Manuel, Vissio, Paula Gabriela
Publicado:	2016
Materias:	fish follicle-stimulating hormone gonadotrophin-inhibitory hormone growth hormone luteinising hormone follitropin follitropin releasing factor gonadotrophin inhibitory hormone gonadotropin luteinizing hormone neuropeptide peptide derivative polypeptide synthetic LPQRFa 1 peptide synthetic LPQRFa 2 peptide synthetic peptide unclassified drug fish protein follitropin beta subunit hypothalamus hormone messenger RNA peptide hormone adult animal cell animal experiment Article brain brain region brain third ventricle cell density cell nucleus Cichlasoma dimerus cichlid controlled study culture medium female follitropin release gonad gonadotropin release growth hormone release hypophysis hypothalamus immunocytochemistry immunoreactivity in vitro study in vivo study male nerve fiber neuromodulation nonhuman olfactory nerve peptide synthesis polymerase chain reaction priority journal protein expression reproduction retina somatic cell animal drug effects genetics metabolism secretion (process) Animals Cichlids Female Fish Proteins Follicle Stimulating Hormone, beta Subunit Gonadotropins Growth Hormone Hypothalamic Hormones Male Peptide Hormones Pituitary Gland RNA, Messenger
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09538194_v28_n5_p_DiYorio http://hdl.handle.net/20.500.12110/paper_09538194_v28_n5_p_DiYorio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09538194_v28_n5_p_DiYorio
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spelling	paper:paper_09538194_v28_n5_p_DiYorio2023-06-08T15:55:33Z Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone Di Yorio, María Paula Somoza, Gustavo Manuel Vissio, Paula Gabriela fish follicle-stimulating hormone gonadotrophin-inhibitory hormone growth hormone luteinising hormone follitropin follitropin releasing factor gonadotrophin inhibitory hormone gonadotropin growth hormone luteinizing hormone neuropeptide peptide derivative polypeptide synthetic LPQRFa 1 peptide synthetic LPQRFa 2 peptide synthetic peptide unclassified drug fish protein follitropin beta subunit gonadotropin growth hormone hypothalamus hormone messenger RNA peptide hormone adult animal cell animal experiment Article brain brain region brain third ventricle cell density cell nucleus Cichlasoma dimerus cichlid controlled study culture medium female follitropin release gonad gonadotropin release growth hormone release hypophysis hypothalamus immunocytochemistry immunoreactivity in vitro study in vivo study male nerve fiber neuromodulation nonhuman olfactory nerve peptide synthesis polymerase chain reaction priority journal protein expression reproduction retina somatic cell animal cichlid drug effects genetics metabolism secretion (process) Animals Cichlids Female Fish Proteins Follicle Stimulating Hormone, beta Subunit Gonadotropins Growth Hormone Hypothalamic Hormones Male Peptide Hormones Pituitary Gland RNA, Messenger The role of gonadotrophin-inhibitory hormone (GnIH) in the inhibition of the reproductive axis has been well-established in birds and mammals. However, its role in other vertebrates, such as the teleost fish, remains controversial. In this context, the present study aimed to evaluate whether GnIH modulates the release of gonadotrophins and growth hormone (GH) in the cichlid fish Cichlasoma dimerus. First, we partially sequenced the precursor polypeptide for GnIH and identified three putative GnIH peptides. Next, we analysed the expression of this precursor polypeptide via a polymerase chain reaction in the reproductive axis of both sexes. We found a high expression of the polypeptide in the hypothalamus and gonads of males. Immunocytochemistry allowed the observation of GnIH-immunoreactive somata in the nucleus posterioris periventricularis and the nucleus olfacto-retinalis, with no differences between the sexes. GnIH-immunoreactive fibres were present in all brain regions, with a high density in the nucleus lateralis tuberis and at both sides of the third ventricle. Finally, we performed in vitro studies on intact pituitary cultures to evaluate the effect of two doses (10−6 m and 10−8 m) of synthetic C. dimerus (cd-) LPQRFa-1 and LPQRFa-2 on the release of gonadotrophins and GH. We observed that cd-LPQRFa-1 decreased β-luteinising hormone (LH) and β-follicle-stimulating hormone (FSH) and also increased GH release to the culture medium. The release of β-FSH was increased only when it was stimulated with the higher cd-LPQRFa-2 dose. The results of the present study indicate that cd-LPQRFa-1, the cichlid fish GnIH, inhibits β-LH and β-FSH release and stimulates GH release in intact pituitary cultures of C. dimerus. The results also show that cd-LPQRF-2 could act as an β-FSH-releasing factor in this fish species. © 2016 British Society for Neuroendocrinology Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Somoza, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vissio, P.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09538194_v28_n5_p_DiYorio http://hdl.handle.net/20.500.12110/paper_09538194_v28_n5_p_DiYorio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	fish follicle-stimulating hormone gonadotrophin-inhibitory hormone growth hormone luteinising hormone follitropin follitropin releasing factor gonadotrophin inhibitory hormone gonadotropin growth hormone luteinizing hormone neuropeptide peptide derivative polypeptide synthetic LPQRFa 1 peptide synthetic LPQRFa 2 peptide synthetic peptide unclassified drug fish protein follitropin beta subunit gonadotropin growth hormone hypothalamus hormone messenger RNA peptide hormone adult animal cell animal experiment Article brain brain region brain third ventricle cell density cell nucleus Cichlasoma dimerus cichlid controlled study culture medium female follitropin release gonad gonadotropin release growth hormone release hypophysis hypothalamus immunocytochemistry immunoreactivity in vitro study in vivo study male nerve fiber neuromodulation nonhuman olfactory nerve peptide synthesis polymerase chain reaction priority journal protein expression reproduction retina somatic cell animal cichlid drug effects genetics metabolism secretion (process) Animals Cichlids Female Fish Proteins Follicle Stimulating Hormone, beta Subunit Gonadotropins Growth Hormone Hypothalamic Hormones Male Peptide Hormones Pituitary Gland RNA, Messenger
spellingShingle	fish follicle-stimulating hormone gonadotrophin-inhibitory hormone growth hormone luteinising hormone follitropin follitropin releasing factor gonadotrophin inhibitory hormone gonadotropin growth hormone luteinizing hormone neuropeptide peptide derivative polypeptide synthetic LPQRFa 1 peptide synthetic LPQRFa 2 peptide synthetic peptide unclassified drug fish protein follitropin beta subunit gonadotropin growth hormone hypothalamus hormone messenger RNA peptide hormone adult animal cell animal experiment Article brain brain region brain third ventricle cell density cell nucleus Cichlasoma dimerus cichlid controlled study culture medium female follitropin release gonad gonadotropin release growth hormone release hypophysis hypothalamus immunocytochemistry immunoreactivity in vitro study in vivo study male nerve fiber neuromodulation nonhuman olfactory nerve peptide synthesis polymerase chain reaction priority journal protein expression reproduction retina somatic cell animal cichlid drug effects genetics metabolism secretion (process) Animals Cichlids Female Fish Proteins Follicle Stimulating Hormone, beta Subunit Gonadotropins Growth Hormone Hypothalamic Hormones Male Peptide Hormones Pituitary Gland RNA, Messenger Di Yorio, María Paula Somoza, Gustavo Manuel Vissio, Paula Gabriela Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone
topic_facet	fish follicle-stimulating hormone gonadotrophin-inhibitory hormone growth hormone luteinising hormone follitropin follitropin releasing factor gonadotrophin inhibitory hormone gonadotropin growth hormone luteinizing hormone neuropeptide peptide derivative polypeptide synthetic LPQRFa 1 peptide synthetic LPQRFa 2 peptide synthetic peptide unclassified drug fish protein follitropin beta subunit gonadotropin growth hormone hypothalamus hormone messenger RNA peptide hormone adult animal cell animal experiment Article brain brain region brain third ventricle cell density cell nucleus Cichlasoma dimerus cichlid controlled study culture medium female follitropin release gonad gonadotropin release growth hormone release hypophysis hypothalamus immunocytochemistry immunoreactivity in vitro study in vivo study male nerve fiber neuromodulation nonhuman olfactory nerve peptide synthesis polymerase chain reaction priority journal protein expression reproduction retina somatic cell animal cichlid drug effects genetics metabolism secretion (process) Animals Cichlids Female Fish Proteins Follicle Stimulating Hormone, beta Subunit Gonadotropins Growth Hormone Hypothalamic Hormones Male Peptide Hormones Pituitary Gland RNA, Messenger
description	The role of gonadotrophin-inhibitory hormone (GnIH) in the inhibition of the reproductive axis has been well-established in birds and mammals. However, its role in other vertebrates, such as the teleost fish, remains controversial. In this context, the present study aimed to evaluate whether GnIH modulates the release of gonadotrophins and growth hormone (GH) in the cichlid fish Cichlasoma dimerus. First, we partially sequenced the precursor polypeptide for GnIH and identified three putative GnIH peptides. Next, we analysed the expression of this precursor polypeptide via a polymerase chain reaction in the reproductive axis of both sexes. We found a high expression of the polypeptide in the hypothalamus and gonads of males. Immunocytochemistry allowed the observation of GnIH-immunoreactive somata in the nucleus posterioris periventricularis and the nucleus olfacto-retinalis, with no differences between the sexes. GnIH-immunoreactive fibres were present in all brain regions, with a high density in the nucleus lateralis tuberis and at both sides of the third ventricle. Finally, we performed in vitro studies on intact pituitary cultures to evaluate the effect of two doses (10−6 m and 10−8 m) of synthetic C. dimerus (cd-) LPQRFa-1 and LPQRFa-2 on the release of gonadotrophins and GH. We observed that cd-LPQRFa-1 decreased β-luteinising hormone (LH) and β-follicle-stimulating hormone (FSH) and also increased GH release to the culture medium. The release of β-FSH was increased only when it was stimulated with the higher cd-LPQRFa-2 dose. The results of the present study indicate that cd-LPQRFa-1, the cichlid fish GnIH, inhibits β-LH and β-FSH release and stimulates GH release in intact pituitary cultures of C. dimerus. The results also show that cd-LPQRF-2 could act as an β-FSH-releasing factor in this fish species. © 2016 British Society for Neuroendocrinology
author	Di Yorio, María Paula Somoza, Gustavo Manuel Vissio, Paula Gabriela
author_facet	Di Yorio, María Paula Somoza, Gustavo Manuel Vissio, Paula Gabriela
author_sort	Di Yorio, María Paula
title	Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone
title_short	Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone
title_full	Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone
title_fullStr	Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone
title_full_unstemmed	Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone
title_sort	gonadotrophin-inhibitory hormone in the cichlid fish cichlasoma dimerus: structure, brain distribution and differential effects on the secretion of gonadotrophins and growth hormone
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09538194_v28_n5_p_DiYorio http://hdl.handle.net/20.500.12110/paper_09538194_v28_n5_p_DiYorio
work_keys_str_mv	AT diyoriomariapaula gonadotrophininhibitoryhormoneinthecichlidfishcichlasomadimerusstructurebraindistributionanddifferentialeffectsonthesecretionofgonadotrophinsandgrowthhormone AT somozagustavomanuel gonadotrophininhibitoryhormoneinthecichlidfishcichlasomadimerusstructurebraindistributionanddifferentialeffectsonthesecretionofgonadotrophinsandgrowthhormone AT vissiopaulagabriela gonadotrophininhibitoryhormoneinthecichlidfishcichlasomadimerusstructurebraindistributionanddifferentialeffectsonthesecretionofgonadotrophinsandgrowthhormone
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Gonadotrophin-Inhibitory Hormone in the Cichlid Fish Cichlasoma dimerus: Structure, Brain Distribution and Differential Effects on the Secretion of Gonadotrophins and Growth Hormone

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