Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats
Hippocampal neuropathology is a recognised feature of the brain in spontaneously hypertensive rats (SHR), but similar studies are lacking in another model of hypertension, the mineralocorticoid-salt-treated rat. The present study aimed to compare changes in hippocampal parameters in 16-week-old male...
Guardado en:
| Publicado: |
2006
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09538194_v18_n6_p466_Pietranera http://hdl.handle.net/20.500.12110/paper_09538194_v18_n6_p466_Pietranera |
| Aporte de: |
| id |
paper:paper_09538194_v18_n6_p466_Pietranera |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_09538194_v18_n6_p466_Pietranera2023-06-08T15:55:33Z Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats Astrocytes Dentate gyrus DOCA-salt hypertension Hippocampus Mineralocorticoids Neurogenesis SHR apolipoprotein E biological marker broxuridine deoxycorticosterone acetate glial fibrillary acidic protein mineralocorticoid receptor sodium chloride analysis of variance animal experiment animal model animal tissue article astrocyte brain damage cell count cell density cell proliferation controlled study dentate gyrus gliosis hippocampus hormone blood level hypertension hypothalamus hypophysis system immunocytochemistry male morphometrics nerve cell nerve cell lesion nonhuman priority journal protein expression rat rat strain sensitivity analysis spontaneously hypertensive rat strain difference Animals Apolipoproteins E Astrocytes Cell Division Dentate Gyrus Desoxycorticosterone Glial Fibrillary Acidic Protein Gliosis Hypertension Male Neurons Rats Rats, Inbred SHR Rats, Inbred WKY Rats, Sprague-Dawley Sodium Chloride Species Specificity Hippocampal neuropathology is a recognised feature of the brain in spontaneously hypertensive rats (SHR), but similar studies are lacking in another model of hypertension, the mineralocorticoid-salt-treated rat. The present study aimed to compare changes in hippocampal parameters in 16-week-old male SHR (blood pressure approximately 190 mmHg) and their normotensive Wistar-Kyoto controls, with those of male Sprague-Dawley rats receiving (i) 10 mg deoxycorticosterone acetate (DOCA) every other day during 3 weeks and drinking 1% NaCl solution (blood pressure approximately 160 mmHg) and normotensive controls treated with (ii) DOCA and drinking water, (iii) drinking water only or (iv) 1% NaCl only. In these experimental groups, we determined: (i) cell proliferation in the dentate gyrus (DG) using the 5-bromo-2′-deoxyuridine-labelling technique; (ii) the number of glial fibrillary acidic protein (GFAP) positive astrocytes under the CA1, CA3 and DG; (iii) the number of apolipoprotein E (ApoE) positive astrocytes as a marker of potential neuronal damage; and (iv) the number of neurones in the hilus of the DG, taken as representative of neuronal density in other hippocampal subfields. Changes were remarkably similar in both models, indicating a decreased cell proliferation in DG, an increased number of astrocytes immunopositive for GFAP and ApoE and a reduced number of hilar neurones. Although hypertension may be a leading factor for these abnormalities, endocrine mechanisms may be involved, because hypothalamic-pituitary function, mineralocorticoid receptors and sensitivity to mineralocorticoid treatment are stimulated in SHR, whereas high exogenous mineralocorticoid levels circulate in DOCA-treated rats. Thus, in addition to the deleterious effects of hypertension, endocrine factors may contribute to the abnormalities of hippocampus in SHR and DOCA-treated rats. © 2006 The Authors. Journal compilation © 2006 Blackwell Publishing Ltd. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09538194_v18_n6_p466_Pietranera http://hdl.handle.net/20.500.12110/paper_09538194_v18_n6_p466_Pietranera |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Astrocytes Dentate gyrus DOCA-salt hypertension Hippocampus Mineralocorticoids Neurogenesis SHR apolipoprotein E biological marker broxuridine deoxycorticosterone acetate glial fibrillary acidic protein mineralocorticoid receptor sodium chloride analysis of variance animal experiment animal model animal tissue article astrocyte brain damage cell count cell density cell proliferation controlled study dentate gyrus gliosis hippocampus hormone blood level hypertension hypothalamus hypophysis system immunocytochemistry male morphometrics nerve cell nerve cell lesion nonhuman priority journal protein expression rat rat strain sensitivity analysis spontaneously hypertensive rat strain difference Animals Apolipoproteins E Astrocytes Cell Division Dentate Gyrus Desoxycorticosterone Glial Fibrillary Acidic Protein Gliosis Hypertension Male Neurons Rats Rats, Inbred SHR Rats, Inbred WKY Rats, Sprague-Dawley Sodium Chloride Species Specificity |
| spellingShingle |
Astrocytes Dentate gyrus DOCA-salt hypertension Hippocampus Mineralocorticoids Neurogenesis SHR apolipoprotein E biological marker broxuridine deoxycorticosterone acetate glial fibrillary acidic protein mineralocorticoid receptor sodium chloride analysis of variance animal experiment animal model animal tissue article astrocyte brain damage cell count cell density cell proliferation controlled study dentate gyrus gliosis hippocampus hormone blood level hypertension hypothalamus hypophysis system immunocytochemistry male morphometrics nerve cell nerve cell lesion nonhuman priority journal protein expression rat rat strain sensitivity analysis spontaneously hypertensive rat strain difference Animals Apolipoproteins E Astrocytes Cell Division Dentate Gyrus Desoxycorticosterone Glial Fibrillary Acidic Protein Gliosis Hypertension Male Neurons Rats Rats, Inbred SHR Rats, Inbred WKY Rats, Sprague-Dawley Sodium Chloride Species Specificity Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats |
| topic_facet |
Astrocytes Dentate gyrus DOCA-salt hypertension Hippocampus Mineralocorticoids Neurogenesis SHR apolipoprotein E biological marker broxuridine deoxycorticosterone acetate glial fibrillary acidic protein mineralocorticoid receptor sodium chloride analysis of variance animal experiment animal model animal tissue article astrocyte brain damage cell count cell density cell proliferation controlled study dentate gyrus gliosis hippocampus hormone blood level hypertension hypothalamus hypophysis system immunocytochemistry male morphometrics nerve cell nerve cell lesion nonhuman priority journal protein expression rat rat strain sensitivity analysis spontaneously hypertensive rat strain difference Animals Apolipoproteins E Astrocytes Cell Division Dentate Gyrus Desoxycorticosterone Glial Fibrillary Acidic Protein Gliosis Hypertension Male Neurons Rats Rats, Inbred SHR Rats, Inbred WKY Rats, Sprague-Dawley Sodium Chloride Species Specificity |
| description |
Hippocampal neuropathology is a recognised feature of the brain in spontaneously hypertensive rats (SHR), but similar studies are lacking in another model of hypertension, the mineralocorticoid-salt-treated rat. The present study aimed to compare changes in hippocampal parameters in 16-week-old male SHR (blood pressure approximately 190 mmHg) and their normotensive Wistar-Kyoto controls, with those of male Sprague-Dawley rats receiving (i) 10 mg deoxycorticosterone acetate (DOCA) every other day during 3 weeks and drinking 1% NaCl solution (blood pressure approximately 160 mmHg) and normotensive controls treated with (ii) DOCA and drinking water, (iii) drinking water only or (iv) 1% NaCl only. In these experimental groups, we determined: (i) cell proliferation in the dentate gyrus (DG) using the 5-bromo-2′-deoxyuridine-labelling technique; (ii) the number of glial fibrillary acidic protein (GFAP) positive astrocytes under the CA1, CA3 and DG; (iii) the number of apolipoprotein E (ApoE) positive astrocytes as a marker of potential neuronal damage; and (iv) the number of neurones in the hilus of the DG, taken as representative of neuronal density in other hippocampal subfields. Changes were remarkably similar in both models, indicating a decreased cell proliferation in DG, an increased number of astrocytes immunopositive for GFAP and ApoE and a reduced number of hilar neurones. Although hypertension may be a leading factor for these abnormalities, endocrine mechanisms may be involved, because hypothalamic-pituitary function, mineralocorticoid receptors and sensitivity to mineralocorticoid treatment are stimulated in SHR, whereas high exogenous mineralocorticoid levels circulate in DOCA-treated rats. Thus, in addition to the deleterious effects of hypertension, endocrine factors may contribute to the abnormalities of hippocampus in SHR and DOCA-treated rats. © 2006 The Authors. Journal compilation © 2006 Blackwell Publishing Ltd. |
| title |
Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats |
| title_short |
Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats |
| title_full |
Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats |
| title_fullStr |
Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats |
| title_full_unstemmed |
Abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats |
| title_sort |
abnormalities of the hippocampus are similar in deoxycorticosterone acetate-salt hypertensive rats and spontaneously hypertensive rats |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09538194_v18_n6_p466_Pietranera http://hdl.handle.net/20.500.12110/paper_09538194_v18_n6_p466_Pietranera |
| _version_ |
1768543430661636096 |